Using 2', 7'-Dichlorodihydrofluorescein-diacetate to Assess Polysaccharides as Immunomodulating Agents

Bland, Elliot James; Keshavarz, Tajalli; Bucke, C.

doi:10.1385/mb:19:2:125

Cited by 17 publications

(18 citation statements)

References 11 publications

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“…Neutrophils were separated from blood by the method of Bland et al [4]. Blood, obtained from the National Blood Service, was collected with informed consent from healthy screened individuals and transferred into sterile disposable universal bottles (Bibby Sterilin, now trading as Barloworld, Scientific, Stone, Staffs., U.K.) containing lipopolysaccharide‐free heparin [20 units/ml (Leo Laboratories, Dublin, Ireland)].…”

Section: Methodsmentioning

confidence: 99%

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Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems

Radman

Bland

Sangworachat

et al. 2006

Biotech and App Biochem

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Use of carbohydrates as elicitors is a novel technique for enhancement of the production of industrially important microbial products. The relation between the levels of ROS (reactive oxygen species) and overproduction of antibiotics in microbial cultures has already been established. In the present study, we aimed to exploit the ROS response to develop a fast technique to assess the potential of oligosaccharides [oligoguluronate, oligomannuronate and MO (mannan oligosaccharides)] and polysaccharides [alginate and LBG (locust-bean gum)] as elicitors for overproduction of secondary metabolites in Streptomyces rimosus and Penicillium chrysogenum. We have also investigated changes in the production of ROS in neutrophils as a result of the action of the same elicitors. LBG-derived oligosaccharides (MO) were most potent inhibitors of ROS in all systems investigated. This correlates with overproduction of secondary metabolites in microbes and enhancement of a number of mammalian systems. We believe that the effects of oligosaccharides and polysaccharides on ROS production by mammalian and microbial cells can be correlated predicatively with overproduction. The underlying methodology offers a fast screening of elicitors that can be applied across the different systems.

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Section: Methodsmentioning

confidence: 99%

“…A method for determining immunoregulation effects of oligosaccharide and polysaccharides has already been developed [4]. Various species of active oxygen produced from dioxygen, i.e.…”

Section: Introductionmentioning

confidence: 99%

Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems

Radman

Bland

Sangworachat

et al. 2006

Biotech and App Biochem

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“…No maximum was set for any well. Further details on the 'DCFH-DA method' can be found in Bland et al 7 …”

Section: Dcfh-da Methodsmentioning

confidence: 99%

“…6 In most cases ROS production is desirable, however in the case of acute respiratory distress syndrome (ARDS), rheumatoid arthritis and ischemia-reperfusion injury the opposite is true. 7 Oligosaccharides such as laminari-oligosaccharides (b-(1 fi 3)-D D -glucans) have been shown to have a variety of effects on the immune system, such as inhibition of cancer metastasis, 8 antitumour activity, 9 immunological activity 10 and complement activation. 11 They are commonly found in higher fungi, plants and seaweeds.…”

Section: Introductionmentioning

confidence: 99%

The influence of small oligosaccharides on the immune system

Bland¹,

Keshavarz²,

Bucke³

2004

Carbohydrate Research

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“…A recent report has shown that a range of carbohydrate elicitors have significant effect on the stimulation and inhibition of ROS in neutrophils. This observation was linked to the structure of the tested oligosaccharides [33].…”

Section: Introductionmentioning

confidence: 99%

Elicitation of plants and microbial cell systems

Radman

Sáez

Bucke

et al. 2003

Biotech and App Biochem

290

122

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Plants show physiological and morphological responses to a range of physical and chemical factors known as 'elicitors'. These responses have been considered as defence reactions 'elicited' by the plants' biochemical factory to ensure their survival, persistence and competitiveness. Recently examples have been cited of elicitation in some fungal and bacterial cultures. Through a chronological survey, this Review considers examples of elicitors and elicitation and describes suggested mechanisms of elicitation in plants and microbial cell cultures. The majority of research in this field has been carried out on the plant systems using complex (undefined) biotic elicitors. Carbohydrates are the main class of compounds used as defined elicitors. This Review focuses on carbohydrates as compounds initiating a defence response in cell cultures. Physiological changes brought about on the plant and microbial cultures include expression of novel metabolites and overproduction of already known products. Recent reports confirming elicitation in microbial cultures are of potential importance, as the relative ease of fermentation and scale-up could open an opportunity for the introduction of useful novel metabolites as well as enhancement of commercially useful bioproducts. In this context, a sound knowledge of the elicitor molecules' structure-function relationships and mechanisms of elicitation is essential.

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Using 2', 7'-Dichlorodihydrofluorescein-diacetate to Assess Polysaccharides as Immunomodulating Agents

Cited by 17 publications

References 11 publications

Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems

Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems

The influence of small oligosaccharides on the immune system

Elicitation of plants and microbial cell systems

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