Using a polyploid meiosis stability (PMeS) line as a parent improves embryo development and the seed set rate of a tetraploid rice hybrid

Zhang, Yachun; Ge, Jie; Wei, Qiong; Jiang, Aiming; Gan, Lu; Song, Zhaoxia

doi:10.4141/cjps09190

Cited by 19 publications

(23 citation statements)

References 26 publications

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“…Unlike Dendranthema nankingense and Dracocephalum kotschyi (Liu et al 2011;Zahedi et al 2014), the total number of flowers per inflorescence, fruits per infructescence and seeds per fruit of the tetraploid J. curcas were all significantly decreased compared with the diploid (Table 4). This may have been because the pollen fertility of the tetraploid was low (He et al 2010(He et al , 2011, or because the tetraploid plants did not produce enough photosynthetic product (Rekika…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of tetraploid and octoploidJatropha curcasinduced by colchicine

et al. 2016

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Jatropha curcas is considered to be one of the greatest potential biofuel plants. However, the application of J. curcas is limited by a lack of high yielding varieties. Polyploids often generate useful germplasm for genetic improvement in many plants. Here, tetraploid and octoploid J. curcas were obtained by treating the shoot tips of seedlings with colchicine. The ploidy of J. curcas was determined by measuring the nuclear DNA content with flow cytometry and by counting chromosomes. Treatment with 0.1% colchicine for seven days was the most effective condition for producing polyploid plants, yielding 15% tetraploids. There was no significant plant height difference between tetraploid and diploid plants, but octoploid plants grew slowly and were about one-third the height of diploid plants. With increased ploidy, the stomata and pollen grains became larger, stomatal density decreased, the leaves became thicker, and the net photosynthetic rate and stomatal conductance decreased. Compared with diploid plants, the tetraploid plants had larger leaves, male and female flowers and pollen, but the octoploid leaves were about half the size of the diploid leaves. The fruits and seeds of the tetraploid plants were larger than those of the diploid, but the single seed weight and oil content were decreased. The octoploid plants were sterile, with no flower buds on three-year-old plants. The polyploid plants obtained in this study could be useful for breeding programs and for studies of flower development and abiotic stress tolerance in J. curcas.

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Section: Resultsmentioning

confidence: 99%

Identification and characterization of tetraploid and octoploidJatropha curcasinduced by colchicine

et al. 2016

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“…At the same time, it is beneficial to improve the effectiveness of polyploidy breeding in the genus Populus, according to the experience of parent selection in hybridization breeding [76]. Polyploidization is essential in speciation, evolution, and creating elite varieties for poplar plantations [77][78][79]. Moreover, triploid Populus is more widely used for the production of lumber, fiber, and fuel, in contrast to diploids [80,81].…”

Section: Discussionmentioning

confidence: 99%

Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus

Zhu

Tian²,

Wang

et al. 2018

Forests

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Poplar is an important research organism, and species in sections Tacamahaca and Aigeiros, have advantages in terms of stress resistance, ease of propagation, and fast growth. Poplar species are widely distributed and well-adapted in the world, presenting a large potential for genetic improvement. Hybridization between different species allows us to generate offspring with a unique combinations of traits. This approach has a huge potential for breeding new poplar varieties that could aid in controlling desertification in the arid and semi-arid zones of the “Three-North” in China. In this study, we carried out a cross test scheme with nine female and thirteen male poplar trees. A total of 105,401 seeds were collected from 117 crosses. Flowering phenology and seed maturation differences of the hybrid progeny were monitored in greenhouses. For male trees, Populus deltoides had the longest flowering time. For female trees, Populus pseudo-simonii showed the longest seed maturity time. The number of carpals and ovules were not the same in different females. Meanwhile, three carpals were found in P. pseudo-simonii. A highly significant positive correlation was found between the seed size and the Thousand Kernel Weight, as well as the seedling cotyledon length. During seed germination, non-radicle and non-hypocotyl seedlings were observed. We also observed a number of cotyledon variants, including single and fused cotyledons, two cotyledons with one cotyledon cracking into two parts, three cotyledons, as well as four cotyledons. These results lay a favorable foundation for combining the research between the sections Tacamahaca and Aigeiros in future work.

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“…As a basic feature in nearly all plant species, polyploidization normally results in a higher yield than that of diploid plants and is regarded as a powerful tool in crop breeding (Ramanna and Jacobsen 2003). During the last decade, the application of polyploidization has been used in rice breeding, and several highly productive hybrid rice lines have been developed (Cai et al 2007;He et al 2011;Ghaleb et al 2020). Through comparative studies between diploid and autotetraploid rice cultivars, it was shown that there are higher glutelin contents in autotetraploid cultivars than in diploid cultivars (Cai 2011).…”

Section: )mentioning

confidence: 99%

iTRAQ-Based Quantitative Glutelin Proteomic Analysis Reveals Differentially Expressed Proteins in the Physiological Metabolism Process during Endosperm Development and their Impacts on Yield and Quality in Autotetraploid Rice

Xian

Long

Yang

et al. 2020

Preprint

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Background: Autotetraploid rice, which is developed through chromosome set doubling using diploid rice, produces high-quality kernels that are rich in storage proteins. However, little information is available about the content of different proteins in autotetraploid rice and their proteomic analysis. Results: The dynamic changes in four storage proteins, albumin, globulin, prolamin, and glutelin, were analyzed in the endosperm of autotetraploid rice (AJNT-4x) and in that of its diploid counterpart (AJNT-2x) for comparison. The contents of the four proteins were all higher during endosperm development in AJNT-4x than in AJNT-2x, but their change and composition were almost the same in the two materials. Then, iTRAQ was employed to analyze the glutelin profiles of AJNT-4x and AJNT-2x at 10 DAF, 15 DAF, and 20 DAF. A total of 1,326 proteins were identified in AJNT-4x and AJNT-2 x using high-throughput LC-MS/MS. Among the 1,326 identified proteins, there were 362 DEPs in AJNT-4x compared with those in AJNT-2x and 372 DEPs between different development stages in AJNT-4x. Eight important upregulated proteins were identified by qRT-PCR, including B8AM24, B8ARJ0, B8AQM6, A2ZCE6, and Q40689. Among them, B8AM24 and B8ARJ0 were related to the lysine biosynthesis process. GO enrichment analysis revealed that the critical functions of DEPs exhibited little overlap between the 10, 15, and 20 DAF groups. Endosperm glutelin accumulation was regulated mainly by different DEPs during the late stage, and 15 DAF was a critical regulating point for glutelin accumulation. KEGG pathway analysis showed that ribosomal proteins were significantly higher in AJNT-4x than in AJNT-2x at 10 DAF, and the protein processing, biosynthesis, and metabolism of amino acids were higher and more active in AJNT-4x at 15 DAF, while the peroxisome was richer in AJNT-4x at 20 DAF. The PPI network showed that ribosomal proteins gradually decreased with increasing endosperm development. Conclusions: These results provide new insights into dynamic glutelin expression differences during endosperm development in autotetraploid rice, which will help in developing rice cultivars with increased yield and improved grain nutritional quality.

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Using a polyploid meiosis stability (PMeS) line as a parent improves embryo development and the seed set rate of a tetraploid rice hybrid

Cited by 19 publications

References 26 publications

Identification and characterization of tetraploid and octoploidJatropha curcasinduced by colchicine

Identification and characterization of tetraploid and octoploidJatropha curcasinduced by colchicine

Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus

iTRAQ-Based Quantitative Glutelin Proteomic Analysis Reveals Differentially Expressed Proteins in the Physiological Metabolism Process during Endosperm Development and their Impacts on Yield and Quality in Autotetraploid Rice

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