Urban tunnels are generally used to measure traffic-related particles and gas pollutant concentrations. To understand on-road vehicle emissions and update emission factors (EFs), traffic volume data and emissions of particulate matter smaller than 2.5 µm (PM 2.5 ), carbon monoxide (CO), nitrogen oxides (NO X ), hydrocarbon (HC), and volatile organic compounds (VOCs) for mixed vehicles were investigated at the Wenchang Gate-Peace Gate Tunnel in Xi'an over 11 days. An average fleet of 14,199 vehicles with mean speeds that ranged from 18 km·h −1 to 46 km·h −1 passed through the tunnel during the sampling period each day. A mass balance model and linear regression analysis were adopted to derive pollutant EFs for mixed vehicles, cars, and taxis, respectively. e results demonstrated that EFs during the night were higher than those during the day because goods vehicles are only allowed to travel from 22:00 to 07:00. Averaged EFs of PM 2.5 , CO, NO X , HC, and VOCs for the total fleet were 0.006 ± 0.005, 1.097 ± 0.398, 0.159 ± 0.092, 0.179 ± 0.089, and 0.317 ± 0.172 g·veh −1 ·km −1 , respectively, lower than those reported from other literatures owing to the strict requirements of emission standards and improvements in vehicle technology. is method provides an approach to measure the EFs for different types of vehicles in urban traffic and evaluate traffic pollution in distinct areas.