Purpose: Physical activity is markedly low in Latina adolescents, yet few physical activity interventions have been attempted in this population. Web-based interventions can incorporate theory-based components, be appealing to adolescents, and have potential for low cost dissemination. Objectives: We sought to assess the feasibility, acceptability, and potential efficacy of a web-based physical activity intervention for Latina adolescents in a single-arm pilot trial. Methods: Twenty-one Latina adolescents (age 12-18) who were underactive (<90 minutes/week) participated in a 12-week theory-informed web-based physical activity intervention. Web content was individually tailored based on responses to monthly questionnaires. Feasibility was measured by recruitment, retention, and adherence/engagement, and acceptability was measured by satisfaction. Physical activity was measured at baseline and follow-up using the 7-Day Physical Activity Recall (PAR) Interview and accelerometers. Results: Baseline activity as measured by the 7-Day PAR and accelerometers was 24.7(26.11) and 24.8(38.3) minutes/week, respectively. At 12 weeks, 19 participants (90.5%) returned. Adherence and engagement with materials was low, and 72% of participants indicated they were satisfied with the intervention. Activity at 12 weeks increased by 58.8(11.33) minutes/week measured by the 7-Day PAR (p<0.001). Accelerometer-measured activity did not increase. Activities reported at follow-up were more varied than at baseline, including some measured poorly by accelerometers (e.g. biking, swimming). Participants suggested simplifying the website and incorporating other technologies. Conclusions: Good retention and increases in self-reported activity suggest a promising approach to delivering a physical activity intervention to Latina adolescents. Incorporating other technologies, such as smartphone apps, could make the intervention more engaging, acceptable and effective.