2010
DOI: 10.1016/j.fsi.2010.02.003
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Using an improved Tol2 transposon system to produce transgenic zebrafish with epinecidin-1 which enhanced resistance to bacterial infection

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Cited by 51 publications
(67 citation statements)
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“…1a) and pTLR-Mylz-EGFP-Mylz-chelonianin (Fig. 1b) were mixed with transposase messenger (m)RNA and then injected into the one-cell stage of zebrafish eggs following our previous report [20]. After microinjection, the eggs were placed in a 28 C incubator, and after 72 h, the presence of green fluorescent color expression was examined by fluorescence microscopy (IX71; Olympus, Tokyo, Japan).…”
Section: Transgene Constructs and Generation Of Muscle-specific Exprementioning
confidence: 99%
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“…1a) and pTLR-Mylz-EGFP-Mylz-chelonianin (Fig. 1b) were mixed with transposase messenger (m)RNA and then injected into the one-cell stage of zebrafish eggs following our previous report [20]. After microinjection, the eggs were placed in a 28 C incubator, and after 72 h, the presence of green fluorescent color expression was examined by fluorescence microscopy (IX71; Olympus, Tokyo, Japan).…”
Section: Transgene Constructs and Generation Of Muscle-specific Exprementioning
confidence: 99%
“…After microinjection, the eggs were placed in a 28 C incubator, and after 72 h, the presence of green fluorescent color expression was examined by fluorescence microscopy (IX71; Olympus, Tokyo, Japan). For further experiments, we chose one transgenic zebrafish to mate with a wild-type (WT) zebrafish, and mating, breeding, and propagation procedures to the F2 generation followed our previous report [20].…”
Section: Transgene Constructs and Generation Of Muscle-specific Exprementioning
confidence: 99%
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“…In fact, the physical mapping of TEs, indispensable for its characterization, are available so far for only 32 fish species [Ferreira et al, 2011b], preventing a broader understanding of their distribution patterns and evolutionary role in phylogenetic analyses. Furthermore, long TEs as Tol1 and Tol2, both belonging to the hAT family of transposons, have provided a potential and powerful tool for genetic studies in transgenesis, mutagenesis, gene trapping, and enhancer trapping [Miskey et al, 2005;Parinov and Emelyanov, 2007;Peng et al, 2010].…”
mentioning
confidence: 99%