2024
DOI: 10.1101/2024.05.27.596136
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UsingCupriavidus necatorH16 to provide a roadmap for increasing electroporation efficiency in non-model bacteria

Matteo Vajente,
Riccardo Clerici,
Hendrik Ballerstedt
et al.

Abstract: Bacteria are a treasure trove of metabolic reactions, but most industrial biotechnology applications rely on a limited set of established host organisms. In contrast, adopting non-model bacteria for the production of various chemicals of interest is often hampered by their limited genetic amenability coupled with their low transformation efficiency. In this study, we propose a series of steps that can be taken to increase electroporation efficiency in non-model bacteria. As a test strain, we useCupriavidus nec… Show more

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