Protecting ecological security has become the backbone of social and economic development since declines in ecological quality due to an increase in human dominance over the natural environment. The establishment of ecological networks is an effective, comprehensive spatial regulation means to ensure regional ecological security. Panzhou city, as a case study, is a typical karst county and has been confronted with the pressure of ecological degradation in recent decades. In this study, an integrated approach combining ecological quality (EQ), ecosystem function importance (EFI), and morphological spatial pattern analysis (MSPA) was developed to determine the ecological sources. Ecological corridors, ecological pinch areas, and ecological barriers were extracted using circuit theory to identify the restored and conserved priority areas of ecological security patterns. The results showed that (1) the remote sensing ecological index (RSEI) and EFI exhibited typical geographical distributions, with the highest values concentrated in the northern and southern parts of the study area and the lowest values scattered in the middle part; (2) 26 patches with forestland, grassland, and waterbodies as the main land cover types were selected as the ecological sources; (3) 63 ecological corridors, composed of 45 key ecological corridors and 18 inactive ecological corridors, were extracted, accounting for 203.12 km and 163.31 km, respectively; (4) 82.76 km2 of pinch areas and 320.29 km2 of barriers were identified, both of which were distributed on key ecological corridors and played different roles in ecological security; and (5) 4 types of ecological security zones were established according to ecological sources, corridors, pinch areas, and barriers. This integrated approach provides a scientific method for the identification and implementation of ecological networks that can contribute to protecting regional ecological security. Our findings can serve as applicable and reasonable guidance to land administrators and policy-makers for adopting suitable territorial spatial planning, urban planning, green cities, etc.