Using magnetic forces to enhance non-viral gene transfer to airway epithelium in vivo

Xenariou, Stefania; Griesenbach, Uta; Ferrari, Stefano; Dean, Philip A. W.; Scheule, Ronald K.; Cheng, Seng H.; Geddes, D M; Plank, Christian; Alton, Eric W.F.W.

doi:10.1038/sj.gt.3302803

Cited by 84 publications

(48 citation statements)

References 36 publications

(47 reference statements)

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“…This might offer an important advantage for ex vivo and in vivo gene delivery, where pDNA is subject to nuclease degradation and systemic clearance. Magnetic gene delivery has been explored for in vivo application in several proof-of-principle experiments (Galuppo et al, 2006;Scherer et al, 2002;Xenariou et al, 2006). The results showed enhanced gene transfer under influence of a magnetic field.…”

Section: Discussionmentioning

confidence: 99%

Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

Pan

Guan

Yoo

et al. 2008

International Journal of Pharmaceutics

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Cationic lipid-coated magnetic nanoparticles (MPs) associated with transferrin were evaluated as gene transfer vectors in the presence of a static magnetic field. MPs were prepared by chemical precipitation and were surface-coated with cationic lipids, composed of DDAB/soy PC (60:40 mole/mole). These cationic MPs were then combined with polyethylenimine (PEI) condensed plasmid DNA, followed by transferrin. The resulting magnetic electrostatic complexes retained relatively compact particle size and showed complete DNA condensation. Their transfection activity in the presence of a static magnetic field was evaluated by luciferase and green fluorescent protein (GFP) reporter genes. The magnetic complexes exhibited up to 300-fold higher transfection activity compared to commonly used cationic liposomes or cationic polymer complexes, based on luciferase assay. The enhancement in transfection activity was maximized when the cells were exposed to the vectors for a relatively short period of time (15 min), or were treated in media containing 10% serum. Incorporation of transferrin further improved transfection efficiency of the cationic MPs. However, when cells were incubated for 4 h in serum-free media, magnetic and non-magnetic vectors showed similar transfection efficiencies. In conclusion, transferrin-associated cationic MPs are excellent gene transfer vectors that can mediate very rapid and efficient gene transfer in vitro in the presence of a magnetic field.

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Section: Discussionmentioning

confidence: 99%

Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

Pan

Guan

Yoo

et al. 2008

International Journal of Pharmaceutics

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“…In this method, called magnetofection, the DNA is reversibly coupled to superparamagnetic nanoparticles which are directed to the target site, following local injection, via a highenergy magnetic field. In vivo magnetofection has been shown to work for small in size plasmid DNA delivery to the gastrointestinal tract of rats and the blood vessels of the ear of pigs (Scherer et al, 2002) and the respiratory epithelium of mice (Xenariou et al, 2006).…”

Section: Other Physical Methodsmentioning

confidence: 99%

Non-Viral Gene Therapy Vectors Carrying Genomic Constructs

Kotzamanis¹,

Abdulrazzak²,

Kotsinas³

et al. 2011

Non-Viral Gene Therapy

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“…TransMAG PEI (Chemicell, Germany), a PEI-coated iron oxide particle, complexed to Genzyme Lipid 67 (GL67) mixed with luciferase plasmid DNA was tested in mouse mammary epithelial cells. Although an increase in gene transfer in vitro was observed, there was no increase in the transfection efficiency in vivo [145]. Biocompatible magnetic nanoparticles enable targeted gene delivery and show much promise for in vitro and in vivo transfection studies.…”

Section: Magnetofectionmentioning

confidence: 97%