Using nanopillars of silicon oxide as a versatile platform for visualizing a selective immunosorbent

Chen, Jem-Kun; Zhou, Gang Yan; Huang, Chih‐Feng; Ko, Fu‐Hsiang

doi:10.1063/1.4812340

Cited by 10 publications

(6 citation statements)

References 26 publications

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“…Leukocytes were employed to examine the non-biofouling properties arising from adsorption upon the anti-EpCAM-modified EBFMs as well as fibrinogen [ 28 , 29 ]. Leukocytes generally generate a significant obstruction for CTC absorption and recognition.…”

Section: Methodsmentioning

confidence: 99%

Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer

et al. 2018

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BackgroundIn addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis.MethodsWe blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption.ResultsCompared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs.ConclusionsThus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.

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Section: Methodsmentioning

confidence: 99%

Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer

et al. 2018

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“…When the NPLs interact specifically or nonspecifically with target molecules, changes typically occur to the geometrical parameters of the gratings and/or the refractive index contrast . In this study, we employed NPL arrays of silica oxide—generated using lithography and reactive ion etching (RIE)—as two‐dimensional periodic relief gratings (2DPRGs) to increase the dimensions of the interface available for DNA adsorption and, thereby, enhance the optical sensitivity . Sequentially, we immobilized oligonucleotides on the surfaces of the NPL arrays and used them to couple with target ssDNA from sample solutions, thereby changing the geometrical parameters (filling factor) and the refractive indices of the gratings .…”

Section: Introductionmentioning

confidence: 99%

Two‐Dimensional Periodic Relief Gratings as a Versatile Platform for Label‐Free Specific DNA Detection

Chen

Zhou

Chang

et al. 2014

Adv Materials Inter

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In this study, nanopillar arrays of silicon oxide are fabricated through a process involving very‐large‐scale integration, for use as two‐dimensional periodic relief gratings (2DPRGs) on silicon surfaces. Oligonucleotides are successively immobilized on the pillar surface, allowing the system to be used as an optical detector specific for the targeted single‐stranded DNAs (ssDNAs). The surfaces of the oligonucleotides‐modified 2DPRGs undergo insignificant structural changes, but upon hybridizing with target ssDNA, the 2DPRGs undergo dramatic changes in terms of their pillar scale. Binding of the oligonucleotides to the 2DPRG occurs in a way that allows them to retain their function and selectively bind the target ssDNA. The performance of the sensor is evaluated by capturing the target ssDNA on the 2DPRGs and measuring the effective refractive index (neff). The binding of the target ssDNA species to the 2DPRGs results in a color change from pure blue to red, observable by the naked eye along an angle of 15–20°. Moreover, effective medium theory is used to calculate the filling factors inside the 2DPRGs and, thereby, examine the values of neff during the structural changes of the 2DPRGs. Accordingly, these new films have potential applications as label‐free optical biosensors.

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“…For increasing the sensitivity of optical biosensors there have been several methods reported with different interrogation techniques [4,5], materials [6,7], and technologies [8,9]. Nanostructure based biosensors have been widely studied to achieve higher sensitivities.…”

Section: Introductionmentioning

confidence: 99%

Silicon nanopillar arrays with SiO_2 overlayer for biosensing application

Choudhury¹,

Casquel²,

Bañuls³

et al. 2014

Opt. Mater. Express

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Abstract:We present the fabrication of silicon dioxide (SiO 2 ) coated silicon nanopillar array structures and demonstrate their application as sensitive optical biosensors. Colloidal lithography, plasma dry etching and deposition processes are used to fabricate SiO 2 coated Si nanopillar arrays with two different diameters and periods. Proof of concept bio recognition experiments are carried out with the bovine serum albumin (BSA)/antiBSA model system using Fourier transform visible and IR spectrometry (FT-VIS-IR) in reflection mode. A limit of detection (LoD) value of 5.2 ng/ml is estimated taking in to account the wavenumber uncertainty in the measurements.

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Using nanopillars of silicon oxide as a versatile platform for visualizing a selective immunosorbent

Cited by 10 publications

References 26 publications

Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer

Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer

Two‐Dimensional Periodic Relief Gratings as a Versatile Platform for Label‐Free Specific DNA Detection

Silicon nanopillar arrays with SiO_2 overlayer for biosensing application

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