Using optimized random amplified polymorphic DNA (RAPD) markers to identify the category status of Citrus nobilis Lour. Gonggan

Abstract

Citrus nobilis Lour. Gonggan is an excellent fruit variety which is widely planted in South China. The origin of Gonggan is not clear. It is conjectured that its origin is from a cross between tangerine and orange; however, there is no direct evidence to confirm this. Here, we applied the optimized random amplified polymorphic DNA (RAPD)-PCR to amplify genus Citrus species: 1) to better understand the genetic relationship between C. nobilis Lour. Gonggan and other Citrus species; and (2) to address the phylogenetic relationship among Citrus species. A total of 21 RAPD primers were used to screen 4 Citrus species and 10 of them efficiently amplified the genomic DNA of 23 Citrus accessions. A total of 87 locus/alleles were generated by those 10 primers with an average of 97.7% polymorphic. Our data supported that C. nobilis Lour. Gonggan belongs to a big group with most tested tangerine and orange and a subgroup with Citrus haniana and Citrus flamea, implying that either C. haniana or C. flamea is likely to be one of the parents of C. nobilis Lour. Gonggan.

Identification of Local Citrus Breeds Using the Start-Codon Targeted Polymorphism Technique Combined with Clonal Sequencing

“…"gonggan" and C. haniana Hort. ex Tseng "Sihuihanggan" have the closest genetic relationship among the local breeds (Ji et al 2012(Ji et al , 2013, consistent with the results of the present study. Some researchers speculate that C. haniana Hort.…”

“…Samples of three citrus-related genera (Citrus, Poncirus, and Atalantia) were selected in this study. The clustering results showed that the SCoT technique revealed genetic diversity among the tested samples, clustering samples of different genera separately, a result largely consistent with previous studies using RAPD and SSR markers (Ji et al 2011c(Ji et al , 2012. In this study, as the hybrid (P. trifoliata × C. sinensis) of Fructus aurantii and sweet orange, citrange (Poncirus trifoliata (L.) Raf.…”

“…The randomness of breed cultivation in different periods has resulted in the loss of citrus genetic resources in the region, especially for some farm varieties. In a previous study on citrus germplasm accessions of Sihui city, we studied the classification status of the local citrus breeds by field cultivation, isozyme analysis, quality analysis, and random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) marker-based analyses of the parental species of existing natural hybrid cultivars based on chloroplast genes (Ji and Guo 2011;Ji et al 2007Ji et al , 2010Ji et al , 2011aJi et al , b, 2012Ji et al , 2013Guo et al 2013).…”