Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers

Lim, Wei Yin; Goh, Choon-Hian; Thevarajah, T. Malathi; Goh, Boon Tong; Khor, Sook Mei

doi:10.1016/j.bios.2019.111792

Cited by 69 publications

(44 citation statements)

References 22 publications

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“…In addition, for DTNB, a characteristic peak was also observed at around 1330 cm À1 (related to the C-N stretching vibrational mode). 16,47,48 The Raman signals of 10 À2 M 4-ATP and DTNB were both weak, whereas strong SERS signals were detected for 4-ATP/DTNB-labeled PDA@Ag-NPs. The analytical enhancement factor (AEF) for PDA@Ag-NPs was calculated using the equation AEF ¼ (I SERS /C SERS )/(I RS /C RS ), where C and I denote the concentration and intensity, and SERS and RS denote the SERS conditions and non-SERS conditions, respectively.…”

Section: Resultsmentioning

confidence: 99%

The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

Liu

Ran

et al. 2020

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

Liu

Ran

et al. 2020

RSC Adv.

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“…Although they achieved LODs of 500 pg/mL for three different cardiac biomarkers, they found that the calibration curve was nonlinear at high concentrations and the LOD varied depending on the detector. In subsequent work [ 30 ], they added Raman reporters to the nanoparticles and used SERS to create a calibration-free device with lower LODs. The improved LODs for the same cardiac biomarkers were 1–10 pg/mL, similar to their clinical cutoff values.…”

Section: Spectroscopic Detectionmentioning

confidence: 99%

Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices

Mitchell

Esene

2021

Anal Bioanal Chem

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Graphical abstract Microfluidic devices can provide a versatile, cost-effective platform for disease diagnostics and risk assessment by quantifying biomarkers. In particular, simultaneous testing of several biomarkers can be powerful. Here, we critically review work from the previous 4 years up to February 2021 on developing microfluidic devices for multiplexed detection of biomarkers from samples. We focus on two principal approaches: electrical and optical detection methods that can distinguish and quantify biomarkers. Both electrical and spectroscopic multiplexed detection strategies are being employed to reach limits of detection below clinical sample levels. Some of the most promising strategies for point-of-care assays involve inexpensive materials such as paper-based microfluidic devices, or portable and accessible detectors such as smartphones. This review does not comprehensively cover all multiplexed microfluidic biomarker studies, but rather provides a critical evaluation of key work and suggests promising prospects for future advancement in this field. Electrical and optical multiplexing are powerful approaches for microfluidic biomarker analysis.

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“…In addition, an ECG examination has high requirements for equipment, which is challenging to meet the needs of POCT. Therefore, it is of great significance to detect cardiac markers with high sensitivity and specificity for early diagnosis of AMI ( Lim et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Point-of-Care Testing for Multiple Cardiac Markers Based on a Snail-Shaped Microfluidic Chip

et al. 2021

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Existing methods for detecting cardiac markers are difficult to be applied in point-of-care testing (POCT) due to complex operation, long time consumption, and low sensitivity. Here, we report a snail-shaped microfluidic chip (SMC) for the multiplex detection of cTnI, CK-MB, and Myo with high sensitivity and a short detection time. The SMC consists of a sandwich structure: a channel layer with a mixer and reaction zone, a reaction layer coated with capture antibodies, and a base layer. The opening or closing of the microchannels is realized by controlling the downward movement of the press-type mechanical valve. The chemiluminescence method was used as a signal readout, and the experimental conditions were optimized. SMC could detect cTnI, CK-MB, and Myo at concentrations as low as 1.02, 1.37, and 4.15. The SMC will be a promising platform for a simultaneous determination of multianalytes and shows a potential application in POCT.

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Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers

Cited by 69 publications

References 22 publications

The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices

Point-of-Care Testing for Multiple Cardiac Markers Based on a Snail-Shaped Microfluidic Chip

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