Using the Liquid Nature of the Stationary Phase: The Elution‐Extrusion Method

Berthod, Alain; Hassoun, Mahmoud; Harris, Guy H.

doi:10.1081/jlc-200063489

Cited by 45 publications

(27 citation statements)

References 5 publications

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“…Under these conditions 50% of stationary phase retention was observed. After that, the whole content of the CPC column was extruded by pumping the organic phase in descending mode at 5 mL min −1 and 500 rpm [27]. The experiment ended after 110 min.…”

Section: Centrifugal Partition Chromatography Fractionationmentioning

confidence: 99%

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Centrifugal partition chromatography elution gradient for isolation of sesquiterpene lactones and flavonoids from Anvillea radiata

Destandau

Boukhris

Zubrzycki

et al. 2015

Journal of Chromatography B

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Section: Centrifugal Partition Chromatography Fractionationmentioning

confidence: 99%

“…Afterward an extrusion step was conducted for 40 min, pumping the fresh organic stationary phase in descending mode until all the injected molecules had been extruded from the column [27]. During extrusion, rotation speed and flow rate were respectively set to 500 rpm and 5 mL min −1 .…”

Section: Germacranolide Purification In Isocratic Modementioning

confidence: 99%

Centrifugal partition chromatography elution gradient for isolation of sesquiterpene lactones and flavonoids from Anvillea radiata

Destandau

Boukhris

Zubrzycki

et al. 2015

Journal of Chromatography B

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“…Up to date, the retention of solute using elution-extrusion CCC method may be predicated as following equations [80,81,90]:…”

Section: Elution-extrusion Counter-current Chro-matographymentioning

confidence: 99%

Counter-Current Chromatography for High Throughput Analysis of Natural Products

Liang

2010

CCHTS

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Counter-current chromatography (CCC) is a unique support-free liquid-liquid partition chromatography winning wide applications in the separation of various components from natural or synthetic mixtures. It has been one of the prime methods for isolating compounds from Traditional Chinese Medicines (TCM) and other comprehensive natural products. Although early CCC models produced a long-standing false image that CCC is a time-consuming technique, rapid and high-performance CCC devices and methods for high-throughput analysis of natural mixtures have been advanced. For instances, multi-channel CCC, dual CCC, elution-extrusion CCC, and solvent simplification protocols can provide high-throughput CCC analysis and produce high purity of compounds or large natural product libraries for drug discovery. This review summarizes the recent advancements of CCC in the high-throughput analysis of natural product with an emphasis on the developments of instruments and methods.

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“…The concentration profile of GL inside the FCPE300 ® column was then determined by extrusion [25]. After loading 200 mg of GL standard into the system, one column volume of the aqueous mobile phase (without KI) was pumped in the descending mode at 10 mL/min.…”

Section: Concentration Profile Of Gl Inside the Fcpe300 ® Column Befomentioning

confidence: 99%

Intensified extraction of ionized natural products by ion pair centrifugal partition extraction

Hamzaoui

Hubert

Hadj-Salem

et al. 2011

Journal of Chromatography A

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Using the Liquid Nature of the Stationary Phase: The Elution‐Extrusion Method

Cited by 45 publications

References 5 publications

Centrifugal partition chromatography elution gradient for isolation of sesquiterpene lactones and flavonoids from Anvillea radiata

Centrifugal partition chromatography elution gradient for isolation of sesquiterpene lactones and flavonoids from Anvillea radiata

Counter-Current Chromatography for High Throughput Analysis of Natural Products

Intensified extraction of ionized natural products by ion pair centrifugal partition extraction

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