Using Ultrafast Responsive Phosphorescent Nanoprobe to Visualize Elevated Peroxynitrite In Vitro and In Vivo via Ratiometric and Time‐Resolved Photoluminescence Imaging

Chen, Zejing; Yan, Peng; Zou, Liang; Zhao, Menglong; Jiang, Jun; Liu, Shujuan; Zhang, Kenneth Yin; Huang, Wei; Zhao, Qiang

doi:10.1002/adhm.201800309

Cited by 39 publications

(17 citation statements)

References 92 publications

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“…Previously, various luminescent probes have been developed for imaging ONOO – in vitro and in vivo. − Some chemical groups have been reported to be employed as the candidate recognition sites for ONOO – . Among them, we noted that the N -dearylation reaction can be specifically triggered by ONOO – in the chemical structure of N -(4-hydroxylphenyl)-rhodol, which is accompanied with the decrease of the conjugation degree of skeleton (Figure a). ,− Accordingly, we speculated that the characteristic of the aforementioned process may be utilized for constructing long-lived phosphorescent probes based on iridium(III) complexes in view of their rich charge-transfer states sensitive to tiny alterations of the structure .…”

Section: Resultsmentioning

confidence: 99%

A Dual-Emissive Phosphorescent Polymeric Probe for Exploring Drug-Induced Liver Injury via Imaging of Peroxynitrite Elevation In Vivo

Chen

Meng

Zou

et al. 2020

ACS Appl. Mater. Interfaces

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Drug-induced liver injury (DILI) is a widespread clinical problem. The pathophysiological mechanisms of DILI are complicated, and the traditional diagnostic methods for DILI have their limitations. Owing to its convenient operation, high sensitivity, and high specificity, luminescent sensing and imaging as an indispensable tool in biological research and clinical trials may provide an important means for DILI study. Herein, we report the rational design and preparation of a near-infrared dual-phosphorescent polymeric probe (P-ONOO) for exploring the DILI via specific imaging of peroxynitrite (ONOO–) elevation in vivo, which was one of early markers of DILI and very difficult to be detected due to its short half-life and high reactive activity. With the utilization of P-ONOO, the raised ONOO– was visualized successfully in the drug-treated hepatocytes with a high signal-to-noise ratio via ratiometric and time-resolved photoluminescence imaging. Importantly, the ONOO– boost in the acetaminophen-induced liver injury in real time was verified, and the direct observation of the elevated ONOO– production in ketoconazole-induced liver injury was achieved for the first time. Our findings may contribute to understanding the exact mechanism of ketoconazole-induced hepatotoxicity that is still ambiguous. Notably, this luminescent approach for revealing the liver injury works fast and conveniently.

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Section: Resultsmentioning

confidence: 99%

A Dual-Emissive Phosphorescent Polymeric Probe for Exploring Drug-Induced Liver Injury via Imaging of Peroxynitrite Elevation In Vivo

Chen

Meng

Zou

et al. 2020

ACS Appl. Mater. Interfaces

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“…Second, the concentration of ONOO – is extremely low and the half-life is extremely short. So, we could believe that the major reason of gel response is NO 2 – …”

Section: Resultsmentioning

confidence: 99%

Nitrite-Responsive Hydrogel: Smart Drug Release Depending on the Severity of the Nitric Oxide-Related Disease

Xiong

Xie

Wang

et al. 2020

ACS Appl. Mater. Interfaces

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Nitric oxide (NO) is known as one of the most important biomarkers of many diseases. However, the development of NO-triggered drug releasing platforms is challenging due to the low concentration and short lifetime of NO in vivo. In this work, a novel nitrite (NO2 –)-responsive hydrogel (DHPL-GEL), which can be used for smart drug release depending on the severity of the NO-related disease, is demonstrated. A dihydropyridine cross-linking agent is designed to construct DHPL-GEL to enable the responsive degradation of the hydrogel triggered by NO2 –. On-demand release of the drug loaded in DHPL-GEL was observed under the stimulation of various concentrations of NO2 – at the physiological level both in vitro and in vivo. In the inflammatory arthritis rat model, the DHPL-GEL drug delivery system showed a better therapeutic effect and less side effects than the traditional therapy and nonresponsive hydrogel drug delivery system, demonstrating the promising application of the NO2 –-responsive hydrogel for the treatment of NO-related diseases.

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“…As shown in Fig. S1, the FTIR spectrum of FITC-MSN shows that the surfactant CTAC was completely removed due to the absence of the characteristic peak of C-N stretching in 3000-2800 cm -1 [49]. The prepared FITC-MSN was well dispersed in water with the averaged size of 53 nm (Fig.…”

Section: Physicochemical Features Of Nanosensormentioning

confidence: 86%

Responsive nanosensor for ratiometric luminescence detection of hydrogen sulfide in inflammatory cancer cells

Liu

Duan

Zhang

et al. 2020

Analytica Chimica Acta

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Gasotransmitter hydrogen sulfide (H2S), produced enzymatically in body, has important functions in biological signaling and metabolic processes. An abnormal level of H2S expression is associated with different diseases, therefore, development of novel bioanalytical methods for rapid and effective detection of H2S in biological conditions is of great importance. In this work, we report the development of a new responsive nanosensor for ratiometric luminescence detection of H2S in aqueous solution and live cells. The nanosensor (Ru@FITC-MSN) was prepared by immobilizing a luminescent ruthenium(II) (Ru(II)) complex into a fluorescein isothiocyanate (FITC) conjugated water-dispersible mesoporous silica nanoparticle (MSN), showing dual emission bands at 520 nm (FITC) and 600 nm (Ru complex). The red luminescence of the formed Ru@FITC-MSN was quenched in the presence of Cu 2+ . The in-situ generated Ru-Cu@FITC-MSN responded to H2S rapidly and selectively, showing a linear ratiometric luminescence change in FITC and Ru(II) channels with the H2S concentration (0.5-4 M). Limit of detection (LoD) and limit of quantification (LoQ) were determined to be 0.36 and 1.21 M. Followed by investigation of cellular uptake processes, the utility of the nanosensor for ratiometric imaging of H2S in live cells and its capability to monitor H2S levels in inflammatory breast cancer cells were then demonstrated. This study provides a powerful approach for detection of highly reactive and unstable H2S biomolecules in live systems.

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Using Ultrafast Responsive Phosphorescent Nanoprobe to Visualize Elevated Peroxynitrite In Vitro and In Vivo via Ratiometric and Time‐Resolved Photoluminescence Imaging

Cited by 39 publications

References 92 publications

A Dual-Emissive Phosphorescent Polymeric Probe for Exploring Drug-Induced Liver Injury via Imaging of Peroxynitrite Elevation In Vivo

A Dual-Emissive Phosphorescent Polymeric Probe for Exploring Drug-Induced Liver Injury via Imaging of Peroxynitrite Elevation In Vivo

Nitrite-Responsive Hydrogel: Smart Drug Release Depending on the Severity of the Nitric Oxide-Related Disease

Responsive nanosensor for ratiometric luminescence detection of hydrogen sulfide in inflammatory cancer cells

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