Abstract-Through the research on collaboration cultivation mechanism of e-commerce applied talents, this paper is to develop cultivation mechanism of opening, sharing, evaluating for electronic commerce talents, to establish collaboration mechanism of education innovation among SMEs, government agencies, institutions of higher education, and to form a stable system of collaborative education and innovation. The research is helpful to build up the collaboration cultivation platform and resource sharing platform of multiple disciplines, majors, centers and systems on campus, to speed up the integration with e-commerce and other industries, and boost economic structure adjustment, industrial transformation and upgrading of traditional enterprises.