Usnic Acid Derivate from <i>Usnea sp.</i> and Bioactivity against <i>Arthemia salina</i> Leach

Abstract

Usnic acid is chemical compound commonly found in lichen. These derivatives compounds both from the synthesis and from the isolation of various lichens have been studied as antibacterial, antifungal, antiparasitic, antiviral, and anticancer. Usnat acid derivative with not hydroxyl group on carbon C-3 was isolated from lichen usnea sp. taken from the Sinjaiselatan described in this paper. Its bioactivity against Arthemia Salina Leach. LC50 value of 19.49 μg/mL.

UHPLC-MS Metabolomic Fingerprinting, Antioxidant, and Enzyme Inhibition Activities of Himantormia lugubris from Antarctica

“…From fraction B (3 g) by medium pressure column chromatography (Kieselgel 60 G, and hexane acetate 80:20 v:v), 335 mg of usnic acid 1 and 33 mg of barbatolic acid 2 were obtained, and from fraction C by chromatography (Kieselgel 60 G, and isocratic hexane acetate 90:10 v:v), 27 mg of 5,7-dihydroxy-6-methylphthalide 3 and 221 mg of atranol 4 were obtained (Figure 2). Usnic acid: (300 MHz, CDCl 3 ): 1.76 (3H, s, Me-13), 2.11 (3H, s, Me-16), 2.66 (3H, s, Me-15), 2.77 (3H, s, Me-18), 5.95 (lH, s, H-4), 11.30 (lH, s, HO-10), 14.36 (lH, s, HO-8), 18.70 (lH, s, HO-3) [55]; barbatolic acid: 1 H-NMR (300 MHz, CDCl 3 -DMSO-d6): 2.44 (3H, s, Me-9), 5.28 (2H, s, -CH 2 -9'), 6.36 (lH, s, H-5), 6.48 (2H, s, H-l', H-5'), 10.34 (lH, s-CHO-8); 5,7-dihydroxy-6-methylphthalide: 1 H-NMR (300 MHz, CDCl 3 ): 2.22 (3H, s, Me), 6.45 (lH, s, arom.-H), 5.35 (CH 2 -), 9.50 (lH, s, -OH), 12.06 (lH, s, -OH) [56]; atranol: 1 H-NMR (300 MHz, CDCl 3 ): 2.31 (3H, s, Me), 6.32 (lH, s, arom. -H), 6.40 (lH, s, -OH), 10.20 (lH, s, CHO), 11.08 (lH, s, -OH) [57].…”