Uterine chromatin template activity during the early stages of pregnancy in the rat

O'grady, J. E.; Moffat, G.E.; McMinn, Linda; Vass, Maureen; O’Hare, Anne; Heald, P. J.

doi:10.1016/0005-2787(75)90279-8

Cited by 9 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The template activity of uterine chromatin which is high on Day 5 of preg¬ nancy and at oestrus (O'Grady et al, 1975) is enhanced by oestradiol and progesterone (Teng & Hamilton, 1968;Glasser & Clark, 1975 , and specific nonhistone chromosomal proteins, which may be important for gene transcription, are detected in uterine cells (Cohen & Hamilton, 1975). New species of nuclear RNA have been found on Day 5 of pregnancy in the rat (Heald & O'Hare, 1973).…”

Section: Discussionmentioning

confidence: 96%

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Surani¹

1977

Reproduction

View full text Add to dashboard Cite

Radiolabelled uterine luminal proteins were obtained 6 h after instillation of 5--10 muCi [35S]methionine into the lumen of each uterine horn of rats. The proteins were analysed electrophoretically on 6% polyacrylamide gels and incorporation of the precursor into the separated proteins was estimated in 0-18 cm gel slices by scintillation spectrometry. Major incorporation was obtained on Day 5 of pregnancy in a group of proteins of approximate mol. wt 3 X 10(4), 4-5 X 10(4) and 5-5 X 10(4) and in proteins of mol. wt 8--10 X 10(4). During pro-oestrus, substantial incorporation of the precursor occurred in a group of protein of mol, wt 13-5 X 10(4), 12-5 X 10(4) and 7-5--8-0 X 10(4) and slight incorporation in proteins of mol. wt 4-5 X 10(4). These changes were mimicked in ovariectomized females by injections of appropriate steroids: progesterone and oestradiol were necessary to induce the proteins detected during pregnancy, oestradiol alone was able to reproduce the changes observed at pro-oestrus. The proteins in the lumen appear to be of uterine origin because radiolabelled serum proteins were not detected in the lumen after an i.p. injection of the isotope. Also, labelled proteins were detected in the horn receiving the isotope but not in the contralateral horn injected with saline. Cycloheximide, given i.p. simultaneously with the isotope but not in the contralateral horn injected with saline. Cycloheximide, given i.p. simultaneously with the isotope, suppressed synthesis of uterine proteins.

show abstract

Section: Discussionmentioning

confidence: 96%

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Surani¹

1977

Reproduction

View full text Add to dashboard Cite

show abstract

“…they were maximal on days 4 and 6 (Bell, Reynolds & Heald, 1976). The changes probably originate from changes on the preceding day in the"uptake of [3H]uridine into RNA (Heald & O'Grady, 1970) Heald, 1975). These variations in the putative factor may serve to regulate uterine sensitivity at critical times.…”

Section: Discussionmentioning

confidence: 97%

Effect of an Intra-Uterine Device on Intracellular Relationships of the Uterine Oestrogen Receptor, Particularly During Pregnancy

Myatt

Chaudhuri

Elder

et al. 1980

Journal of Endocrinology

View full text Add to dashboard Cite

The presence of an intra-uterine device in the rat results in a lower nuclear concentration of the oestrogen receptor in the treated horn at pro-oestrus when it is compared with the contralateral control horn. This effect was also seen after the administration of hyperphysiological doses of oestradiol and when the horn was exposed in vitro to high concentrations of oestradiol. The cyclic changes during the oestrous cycle in the activity of the oestrogen-induced enzyme peroxidase were similar in the treated and control horns. These observations have discounted the possibility that the relatively lower nuclear receptor content in the treated horn at pro-oestrus was due to a decreased exposure to oestrogen. A significantly lower nuclear content was also observed in the treated horn on days 4 and 5 of pregnancy. This was not associated with a deficiency in cytosol receptor content which increased concurrently with that of the control horn in the 6 days of pregnancy that were studied. The proportional content of the putative cytosol factor implicated in receptor translocation was similar in both horns, increasing on days 4 and 6 in concert with reported changes in 'induced protein' synthesis. There appeared to be reduced levels of nuclear receptor at a time when blastocyst implantation normally occurs.

show abstract

“…In the pregnant rat oestradiol secretion increases markedly between 22.00 h on Day 3 and 04.00 h on Day 4 of pregnancy (Watson, Anderson, Alam, O'Grady & Heald, 1975) (Watson et al, 1975), but it may be related to the intense stromal mitosis occurring on that day (Lobel, Levy &Shelesnyak, 1967;Dupont-Mairesse & Galand, 1975 O'Grady et al, 1975) and the decidual response is undetectable. The increase in protein synthesis in the decidualizing tissue but not in the inter-implantation areas is interesting, and work is continuing to examine the relationship between these events more closely.…”

Section: Resultsmentioning

confidence: 99%

Uterine protein synthesis during the early stages of pregnancy in the rat

Bae¹,

Reynolds²,

Heald³

1977

Reproduction

View full text Add to dashboard Cite

show abstract

Uterine chromatin template activity during the early stages of pregnancy in the rat

Cited by 9 publications

References 21 publications

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Effect of an Intra-Uterine Device on Intracellular Relationships of the Uterine Oestrogen Receptor, Particularly During Pregnancy

Uterine protein synthesis during the early stages of pregnancy in the rat

Contact Info

Product

Resources

About