Uterine endometrial vascularization during ovarian follicular growth in llamas: The effect of estradiol plasma concentration

Silva, Mauricio; Urra, Felipe; Ratto, M.H.

doi:10.1016/j.theriogenology.2017.10.027

Cited by 9 publications

(8 citation statements)

References 40 publications

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“…In the same way, in the present study, luteal vascularization in all llamas increased from Day 6 towards Day 8 post‐mating and then decreased in non‐pregnant animals, while it remained elevated in the pregnant ones; resembling plasma P4 profile. Similarly, in non‐pregnant llamas, both plasma P4 and luteal vascularization increased from treatment to Day 8 after pharmacological induction of ovulation and then decreased between Days 10 and 12 (Silva et al, ; Ulloa‐Leal et al, ). Also in non‐pregnant alpacas, luteal vascularization increased after induction of ovulation, reaching its maximum values between Days 7 and 9 after GnRH analog (Gonadorelin acetate) administration and then decreased in coincidence with plasma P4 concentration (Norambuena et al, ).…”

Section: Discussionmentioning

confidence: 98%

“…Occurrence of ovulation was assessed 2 days after mating based on ultrasonographic visualization of the disappearance of the dominant follicle and further confirmed by P4 plasma profile. Afterwards, females were examined by transrectal doppler ultrasonography (MyLab 30 Vet Gold ESAOTE Pie medical attached to a 5.0 MHz lineal‐array electronic transducer) every other day from Day 6 until Day 18 post‐mating; in order to asses CL's diameter and vascularization, as described for several species (mare: Ginther & Utt, ; cattle: Viana et al, ; alpaca: Norambuena et al, ; llama: Silva, Urra, & Ratto, ). Briefly, to asses CL vascularization, the transducer was placed over the ovary and three seconds video clips of the CL vascularization was registered and downloaded.…”

Section: Methodsmentioning

confidence: 99%

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Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Gallelli

Bianchi

Zampini

et al. 2019

Reprod Domestic Animals

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The aim of this study was to characterize corpus luteum vascularization and its association with plasma progesterone concentration in early stages of pregnancy, when maternal recognition of pregnancy is expected to occur. In all animals, both plasma progesterone concentration and corpus luteum vascularization increased from Day 6 to Day 8 post‐mating and afterwards in non‐pregnant llamas they started to decrease to reach basal levels around Days 12 to 14 post‐mating, while in pregnant animals, both variables remained elevated until the end of the study. A lineal positive relationship between corpus luteum vascularization and plasma progesterone concentration was observed in pregnant (r2 = .46, p < .0001) and non‐pregnant llamas (r2 = .66, p < .0001). Pregnant animals showed higher plasma progesterone concentration and corpus luteum vascularization than the non‐pregnant ones from Day 12 post‐mating until the end of the study (p ˂ .05 and p ˂ .01, respectively). These results suggest that maternal recognition of pregnancy should occur before Day 12 post‐mating in order to expand luteal lifespan, maintaining corpus luteum vascularization and progesterone production. Also, the assessment of CL vascularization area could be a useful and non‐invasive method for early pregnancy diagnosis due to its association with plasma progesterone concentration.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Gallelli

Bianchi

Zampini

et al. 2019

Reprod Domestic Animals

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“…Se dispone de reportes donde el intervalo a la emergencia de una nueva onda folicular como respuesta a la ablación folicular se presentó a los 3.1 ± 0.9 días con base a ecografías diarias (Ratto et al, 2003) y de 1.8 ± 0.4 días con ecografías cada 12 horas (Silva et al, 2018). Asimismo, Andrade (2007), con la aplicación de GnRH reportó el desarrollo de folículos >3 mm a los 4.6 ± 1.2 días.…”

Section: Discussionunclassified

Efecto de la aplicación de plasma seminal, GnRH y ablación folicular sobre la dinámica folicular en llamas (Lama glama)

Silva

Coaguila

Medina

et al. 2020

Rev. investig. vet. Perú

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El presente estudio tuvo como objetivo evaluar el efecto de la aplicación de plasma seminal, un análogo de GnRH y la ablación folicular sobre la dinámica folicular en llamas. El estudio se realizó en Puno, Perú, utilizando 24 llamas seleccionadas bajo el criterio de presentar un folículo preovulatorio >7 mm, determinado mediante ecografía transrectal. Las llamas fueron distribuidas aleatoriamente en cuatro grupos: T1, 1 ml PBS IM; T2, 2 ml plasma seminal IM; T3, 1 ml GnRH IM; T4, ablación folicular. Los animales fueron evaluados, mediante ecografía transrectal, cada 2 h entre las 20 hasta las 36 h post-tratamiento para determinar la ovulación; posteriormente en forma diaria hasta el día 9 y luego cada 2 días hasta el día 15 pos-estímulo para determinar el intervalo a la emergencia de una nueva onda folicular, la aparición del nuevo folículo dominante y el diámetro del cuerpo lúteo al día 8 del tratamiento. No se encontró diferencia significativa (p<0.05) entre tratamientos en las variables en estudio. Es posible que existan otros factores que podrían afectar el intervalo a la emergencia de una nueva onda folicular.

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“…The settings (B-mode frequency: 5 MHz with a depth of 8 cm and a gain of 52%; CFM pulse repetition frequency: 1.4 KHz and a gain of 70%) were standardized and remained constant for all examinations. Briefly, the transducer was placed over the middle segment of each uterine horn (UH) as described in llamas (18,19), to display signals for blood flow in the vessels of all the endometrium, myometrium, and perimetrium, and over the CL. A 3 s videoclip of the vascularization of each structure was registered and downloaded.…”

Section: Uterine and Corpus Luteum Vascularizationmentioning

confidence: 99%

“…Whereas, if ovulation occurs in the left ovary, the embryo would not need to migrate because the right uterine horn is incapable of lysing a CL in the left ovary (11,20,36). Estradiol has a potent vasodilator effect (18,38,39) and it is possible that for this reason, the females in Group I+ with a CL in the left ovary register higher levels of blood flow in the ipsilateral uterine horn. However, increasing the number of animals evaluated could take this difference in UBF% between uterine horns to become statistically significant.…”

Section: Figure 4 |mentioning

confidence: 99%

Uterine and Corpus Luteum Blood Flow Evaluation Prior to Uterine Flushing in Llama Embryo Donors

et al. 2020

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The aim of this study was to assess the uterine blood flow (UBF) and corpus luteum blood flow (CLBF) in llamas 8 days post-mating, using color-Doppler ultrasonography (CDU), to determine the possible relationship between vascularization and the presence of an embryo. Adult females ( n = 25) were used to monitor ovarian dynamics by palpation and transrectal ultrasonography until detection of a ≥6 mm growing follicle. Females were randomly assigned to one of two groups: Group I ( n = 19), were mated and ovulation was induced by a single dose of buserelin (GnRH analog) that same day (Day 0); and Group II ( n = 6), only ovulation was induced (control). On Day 8, UBF and CLBF were evaluated transrectally in both groups. The color-flow images obtained were analyzed with Image J1.52a software to determine the vascularization area and the percentage of corpus luteum with blood flow emission (CLBF%) together with the percentage for each uterine horn (UBF%). Statistical analysis was performed using an ANOVA test. In Group I, uterine flushing was performed to obtain the embryos, thus dividing the females into Group I+ ( n = 10), when an embryo was recovered and Group I- ( n = 9), when no embryo was recovered. Embryo recovery rate was 52.63% (10/19). In Group I+, UBF% was significantly higher compared to Group I- and Group II ( P <0.05). UBF appears to be a good predictor for embryo presence, with an area under the curve (AUC) of 0.9 and an optimal cut-off value of 9.37% (with a sensitivity of 90% and specificity of 88.9%). The CLBF% did not differ between groups ( P > 0.05). In conclusion, it is possible to detect a local increase of UBF in the presence of an embryo on day 8 post-mating in llamas. This could be useful to achieve an early pregnancy diagnosis or to decide whether to carry out the uterine flushing in a llama embryo transfer program.

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Uterine endometrial vascularization during ovarian follicular growth in llamas: The effect of estradiol plasma concentration

Cited by 9 publications

References 40 publications

Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Efecto de la aplicación de plasma seminal, GnRH y ablación folicular sobre la dinámica folicular en llamas (Lama glama)

Uterine and Corpus Luteum Blood Flow Evaluation Prior to Uterine Flushing in Llama Embryo Donors

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