Uterine growth responses of the mature castrate rat to estradiol-17B

Lavia, Lynn A.; Shideler, C.; Parley, N.; Walker, N; Fields, W. Calvin; Roberts, Daniel K.

doi:10.1016/0039-128x(84)90029-1

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…Stromal oedema was microscopically evident after 1 day of estradiol treatment ( Figure 2b). These findings established that the treatment used had the effect on uterus expected from a high dose estradiol regimen (Lavia et al, 1984;Lee, 1972;Martin et al, 1973;Stormshak et al, 1976).…”

Section: Methodsmentioning

confidence: 70%

Differential expression of cAMP-kinase subunits is correlated with growth in rat mammary carcinomas and uterus

Houge

Cho-Chung²,

Døskeland³

1992

Br J Cancer

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SummaryThe expression of the regulatory (RI and RII) and catalytic (C) subunits of cAMP-dependent protein kinase was found to depend on the growth-state in oestrogen-dependent DMBA-induced mammary adenocarcinomas as well as in uteri of the rat. Castration-induced atrophy of the oestrogen-dependent tissues was accompanied by a decrease of the concentration of regulatory subunits (RI and RII) relative to both the catalytic subunit (C) and total protein, decreasing the R/protein and R/C ratios. A hyperplastic burst caused by high-dose oestrogen-replacement treatment was associated with an increased level of RI and little change in RII and C levels. Only minor differences were noted for the expression of mRNA for the a and P subtypes of RI, RII and C between rat uteri from castrated and oestrogen-treated animals, or between mammary tumours from normal and castrated animals. Expression of RIP-mRNA was detected only in the uterus. Our findings provide an experimental correlate for the reported value of the parameter R/protein in human mammary cancer biopsies to predict prognosis and outcome of therapy. Due to the sensitivity of the R/protein ratio towards changes in extracellular protein content, we recommend the biologically more meaningful R/C ratio in further clinical evaluations of mammary tumour biopsies.

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Section: Methodsmentioning

confidence: 70%

Differential expression of cAMP-kinase subunits is correlated with growth in rat mammary carcinomas and uterus

Houge

Cho-Chung²,

Døskeland³

1992

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…These doses were chosen based on previous studies (Lavia et al 1984, Mouihate & Pittman 2003. Two days after this surgery, the ovariectomized rats began to receive ECP (Pfizer, Paulínia, SP, Brazil) hormone replacement, subcutaneously for three consecutive days at two different doses, 10 or 25 lg per animal in 0.1 mL.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…Two days after this surgery, the ovariectomized rats began to receive ECP (Pfizer, Paulínia, SP, Brazil) hormone replacement, subcutaneously for three consecutive days at two different doses, 10 or 25 lg per animal in 0.1 mL. These doses were chosen based on previous studies (Lavia et al 1984, Mouihate & Pittman 2003. The control groups (Oil) received sc injection of corn oil, which was the vehicle to dilute the ECP.…”

Section: Experimental Protocolsmentioning

confidence: 99%