Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum

Pfeifer, Luiz Francisco Machado; Andrade, Jéssica de Souza; Moreira, Elizângela Mírian; Silva, Renata Reis da; Neves, P. C. F.; Silva, George Moreira da; Lemos, Izabela Cristina; Schneider, Augusto

doi:10.1016/j.anireprosci.2018.08.039

Cited by 17 publications

(18 citation statements)

References 50 publications

(63 reference statements)

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“…** and *** indicate P<0.01 and P<0.001 vs. the LPS group, respectively. CG: control group; LPS: lipopolysaccharide; DXM: dexamethasone; PBS: phosphate-buffered saline; bEEC: bovine endometrial epithelial cell; TLR4: Toll-like receptor 4 disrupt the resumption of cyclic ovarian function (Sicsic et al, 2018), and cause failure of artificial insemination and infertility, thereby causing huge economic losses (Machado Pfeifer et al, 2018). The endometrium is the first line of defense against reproductive tract infections after delivery (Shen et al, 2018).…”

Section: Fig 7 Effect Of Catalpol On Endometrial Inflammation Of Lpsmentioning

confidence: 99%

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Zhang

Yang

et al. 2019

J. Zhejiang Univ. Sci. B

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Catalpol is the main active ingredient of an extract from Radix rehmanniae, which in a previous study showed a protective effect against various types of tissue injury. However, a protective effect of catalpol on uterine inflammation has not been reported. In this study, to investigate the protective mechanism of catalpol on lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and mouse endometritis, in vitro and in vivo inflammation models were established. The Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway and its downstream inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), western blot (WB), and immunofluorescence techniques. The results from ELISA and qRT-PCR showed that catalpol dose-dependently reduced the expression of pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin (IL)-1β, and IL-6, and chemokines such as C-X-C motif chemokine ligand 8 (CXCL8) and CXCL5, both in bEECs and in uterine tissue. From the experimental results of WB, qRT-PCR, and immunofluorescence, the expression of TLR4 and the phosphorylation of NF-κB p65 were markedly inhibited by catalpol compared with the LPS group. The inflammatory damage to the mouse uterus caused by LPS was greatly reduced and was accompanied by a decline in myeloperoxidase (MPO) activity. The results of this study suggest that catalpol can exert an anti-inflammatory impact on LPS-induced bEECs and mouse endometritis by inhibiting inflammation and activation of the TLR4/NF-κB signaling pathway.

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Section: Fig 7 Effect Of Catalpol On Endometrial Inflammation Of Lpsmentioning

confidence: 99%

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Zhang

Yang

et al. 2019

J. Zhejiang Univ. Sci. B

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“…Uterine involution and mechanisms leading to uterine disease (and infertility) are essentially different between dairy and beef cows. Overall, there is limited peer‐reviewed literature on beef cattle endometritis, and the available information is often conflicting (Machado Pfeifer et al, 2018; Ricci et al, 2015; Ricci et al, 2017; Santos et al, 2009). This is because diagnostic criteria established by dairy research are simply extrapolated to beef cows.…”

Section: Importance and Classificationmentioning

confidence: 99%

General and comparative aspects of endometritis in domestic species: A review

Pascottini

Aurich

Grahofer

2023

Reprod Domestic Animals

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Endometritis is a leading cause of sub‐ and infertility in domestic animal species. The healthy uterus is colonized by commensal bacteria, viruses and yeast/fungi that represent the nonpathogenic microbiota. A shift in the number or type of organisms accompanied by immune dysfunction, however, may trigger uterine infection and inflammation. Metritis is associated with inflammation of all uterine layers (endometrium, myometrium and perimetrium), whereas endometritis is a more superficial inflammation involving solely the endometrium. Endometritis generally occurs at two time points in domestic animal species, postpartum and postmating. Postpartum endometritis may chronically persist, either as a low‐grade disease that often manifests as a vaginal discharge but not a systemic illness (in some species termed clinical endometritis) or sometimes subclinical where features are only detected by endometrial sampling. Contamination of the uterus at the time of mating occurs by direct deposition of semen (ejaculated or artificially inseminated) into the uterus. Improper drainage of the ejaculatory fluid or an inadequate immune response may result in persistent mating‐induced endometritis. Both postpartum and postmating endometritis interferes with fertility by creating a suboptimal environment for embryo development and placentation, and chronic endometritis may have an impact on sperm survival and fertilization ability. In the postpartum animal, there may also be changes in milk production and maternal behaviour, which can affect offspring health and survival. Preventive strategies for endometritis largely depend on monitoring their known risk factors, which are sometimes specific with regard to the species. Effective, nonantibiotic therapy for endometritis is not available to date. Overall, extensive research has been performed in cattle and horses to unravel key aspects of endometritis, but in sows and bitches, the available literature is scant. Thus, the need and opportunity to investigate the condition vary considerably among domestic species and necessitate their comparative assessment. This article reviews general and comparative aspects of the diagnosis and classification, pathogenesis, preventive strategies and therapeutics of endometritis in domestic species with a specific focus on cows, mares, sows and bitches.

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“…However, if the placenta or necrotic tissues are not properly expelled, inflammation and/or bacterial infections may occur leading to uterine diseases such as metritis or clinical and subclinical endometritis (Azawi, 2008;Sheldon et al, 2008). Most endometritis research has focused on dairy cattle, however the prevalence of subclinical endometritis in postpartum beef cattle can range from 34 to 88% within a herd (Santos et al, 2009;Ricci et al, 2017;Machado Pfeifer et al, 2018). Before 50 DPP (week 7), pregnancy rates are reduced to <40% and after 50 DPP, pregnancy rates greatly improve, even with ∼15% of cows still affected with subclinical endometritis (Santos et al, 2009;Ricci et al, 2017;Machado Pfeifer et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Most endometritis research has focused on dairy cattle, however the prevalence of subclinical endometritis in postpartum beef cattle can range from 34 to 88% within a herd (Santos et al, 2009;Ricci et al, 2017;Machado Pfeifer et al, 2018). Before 50 DPP (week 7), pregnancy rates are reduced to <40% and after 50 DPP, pregnancy rates greatly improve, even with ∼15% of cows still affected with subclinical endometritis (Santos et al, 2009;Ricci et al, 2017;Machado Pfeifer et al, 2018). The presence of pathogenic bacteria from any infection in the uterus is detected by the innate immune system.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Reproductive Tract Cytokines in Post-partum Beef Cows Relating to Reproductive Microbiota and Fertility Outcomes

et al. 2021

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The activity of the immune system in the reproductive tract has been proven to be crucial in the response to uterine diseases, normal reproductive functions, and tolerance to the allogeneic fetus during pregnancy. The objectives of the current study were to (1) evaluate uterine and vaginal cytokine concentrations in postpartum cows undergoing estrus synchronization followed by timed artificial insemination (TAI) and (2) correlate bacterial communities with cytokine concentrations. Postpartum Angus cows (n = 20) were subjected to a 7-Day Co-Synch protocol with pre-synchronization beginning 21 days prior (d −21) to TAI (d 0). Uterine and vaginal flushes were collected on d −21 and −2. Pregnancy was determined by transrectal ultrasound on d 30. Cytokines include interleukin (IL)-1b, IL-6, IL-10, transforming growth factor beta (TGF-β), and immunoglobin A (IgA) and concentrations were determined by commercial ELISA kits. No differences by day or pregnancy status in cytokine concentrations were detected in vaginal samples. No differences by day or pregnancy status in IgA, IL-10, or IL-1b concentrations were detected in uterine samples. Overall TGF-β concentrations in the uterus were greater in resulting pregnant than non-pregnant cows (44.0 ± 13.4 pg/mL vs. 14.7 ± 4.9 pg/mL; P = 0.047). Uterine TGF-β was correlated with the relative abundance of genera Treponema (r = −0.668; P = 0.049) in resulting non-pregnant cows on d −21 and with the relative abundance of genera Ureaplasma (r = 0.901; P = 0.0004) in resulting pregnant cows on d −2. In resulting pregnant animals, a tendency for a strong correlation was detected between d −2 progesterone concentrations and uterine TGF-β concentrations (r = 0.591, P = 0.07). Overall IL-6 concentrations in the uterus were greater in resulting non-pregnant than pregnant cows (198.7 ± 21.8 pg/mL vs. 144.3 ± 16.1 pg/mL; P = 0.045). A correlation was also detected between uterine IL-6 concentrations and the relative abundance of genera Butyrivibrio (r = 0.742; P = 0.022) in resulting non-pregnant cows on d −21. These results suggest possible relationships between different bacterial communities and cytokine concentrations within the uterus of beef cattle prior to TAI that may ultimately affect fertility outcomes.

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Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum

Cited by 17 publications

References 50 publications

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

General and comparative aspects of endometritis in domestic species: A review

Evaluation of Reproductive Tract Cytokines in Post-partum Beef Cows Relating to Reproductive Microbiota and Fertility Outcomes

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