Utilisation of glycogen, ATP and arginine phosphate in exercise and recovery in terrestrial red crabs, Gecarcoidea natalis

Morris, Stephen A.; Adamczewska, Agnieszka M.

doi:10.1016/s1095-6433(02)00217-9

Cited by 32 publications

(28 citation statements)

References 37 publications

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“…At the point of sacrifice, related metabolites revealed that an advanced glycolysis had taken place even before death occurred and haemolymph circulation ceased. This is consistent with the fact that exercise and emersion are both known to trigger anaerobic glycolysis in crustaceans (Taylor & Spicer 1987;Spicer et al 1990;Morris & Adamczewska 2002). In N. norvegicus it has been shown by several authors that during high stress events (e.g.…”

Section: Immediate Effects Of Stress On Stress-related Metabolite Valuessupporting

confidence: 88%

“…This is analogous to observations in other crustaceans following exercise, e.g. in the prawn Palaemon serratus tail muscle (Thébault et al 1997) and the crab Gecarcoidea natalis (Morris & Adamczewska 2002) or during hypoxia in the prawns Palaemon elegans and P. serratus (Taylor & Spicer 1987). Interestingly, the effect of emersion on the AEC seems to be speciesdependent, as Carlsson & Gäde (1986) could show that hypoxia in the horseshoe crab Limulus polyphemus (Linnaeus, 1758) (Chelicerata, Xiphosura) did not affect the AEC in muscle over 48 h.…”

Section: Immediate Effects Of Stress On Stress-related Metabolite Valuessupporting

confidence: 86%

“…The same has been observed in other species of arthropods. The horseshoe crab Limulus polyphemus, the terrestrial red crabs Gecarcoidea natalis, and the Australian yabby (Cherax destructor Clark, 1936), for example, use AP i during exercise and tail-flipping (Carlsson & Gäde 1986;Morris & Adamczewska 2002).…”

Section: Immediate Effects Of Stress On Stress-related Metabolite Valuesmentioning

confidence: 99%

“…Herreid & Full 1986). Morris & Adamczewska (2002) showed that brief exercise in the terrestrial air-breathing crab Gecarcoidea natalis (Pocock, 1888) depletes arginine phosphate stores, consumes glycogen and results in the accumulation of L-lactate within the walking leg muscles. The tail-flip escape swimming of the spiny lobster Jasus edwardsii (Hutton, 1875) induces extensive anaerobic glycolysis in the abdominal muscles (Speed et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster,Nephrops norvegicus(Linnaeus, 1758)

Gornik

Albalat

Atkinson³

et al. 2010

Marine Biology Research

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The effects of four different ante-mortem stressors (exercise, emersion, starvation and a patent infection with the parasite Hematodinium sp.) on post-mortem processes have been investigated in the abdominal muscle of Norway lobster Nephrops norvegicus by measuring changes in the pH, the levels of glycogen, L-lactate, arginine phosphate, ATP, ADP, AMP, IMP, HxR, Hx and the adenylate energy charge (AEC) over a time course of 24 h with samples being taken at 0, 3, 6, 12 and 24 h. The acute stresses of intense exercise and 2 h emersion resulted in a premature onset of anaerobic glycolysis, leading both to an enhanced glycogen depletion rate and an early accumulation of L-lactate. The chronic stressors, starvation and parasite infection, resulted in a complete ante-mortem depletion of muscle glycogen and consequently the failure of post-mortem glycolytic fermentation. Post-mortem pH and ATP inter-conversion were significantly altered in chronically stressed animals. Ante-mortem, a rapid, almost complete depletion of arginine phosphate was observed in all stress groups. The AEC was altered significantly by all stresses, indicating a strong energy demand. The findings suggest that ante-mortem stressors strongly influence the post-mortem biochemical processes. The laboratory-based results are compared to 'field' data and effects on post-harvest product quality are discussed.

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Section: Immediate Effects Of Stress On Stress-related Metabolite Valuessupporting

confidence: 88%

Section: Immediate Effects Of Stress On Stress-related Metabolite Valuessupporting

confidence: 86%

Section: Immediate Effects Of Stress On Stress-related Metabolite Valuesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster,Nephrops norvegicus(Linnaeus, 1758)

Gornik

Albalat

Atkinson³

et al. 2010

Marine Biology Research

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show abstract

“…The crustacean physiological response to functional and environmental hypoxia is similar to that in vertebrates. When the intracellular phosphagen buffer (arginine phosphate in crustaceans) is exhausted, anaerobic glycogenolysis is employed, and lactic acid fermentation occurs (Booth & McMahon, 1985;England & Baldwin, 1983;Gornik et al, 2008;Johnson et al, 2004;Morris & Adamczewska, 2002). Conversion of pyruvate to lactic acid enables the restoration of NAD , which is essential for continued glycolysis under hypoxic conditions.…”

Section: Introductionmentioning

confidence: 99%

Lactate accumulation in the intertidal hermit crab, <i>Pagurus samuelis</i>, in response to burial-induced hypoxia

2017

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Abstract. We subjected the intertidal hermit crab, Pagurus samuelis (Stimpson, 1857), to various treatments to determine physiological responses of this species to the environmental stress of burial. Hermit crabs were buried with 6 cm of sediment and excavated at 2 h intervals up to a maximum of 12 h. Duration of burial and state (alive or dead) of the crab were analyzed for effects on lactate accumulation in hemolymph. Hermit crab weight, shell weight, weight ratio, lactate, and burial duration were analyzed for their influence on survival. As expected, lactate levels, as well as incidence of death, rose with duration of burial. Significant interaction, however, was also found between burial duration, crab state, and lactate concentration. There was a trend for lactate concentration to be low for surviving crabs, yet higher for dead crabs during shorter burial durations. Conversely and surprisingly, lactate concentrations were very high in surviving crabs, yet lower in dead crabs during long burial durations. Since some surviving crabs were able to develop very high lactate levels, we suggest that lactate buildup itself is unlikely to be the sole cause of death. Further studies are needed to identify factors affecting crab resilience during the stress of burial.

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Biocatalytic Phosphorylation of Metabolites

Gauss¹,

Schönenberger²,

Molla

et al. 2016

Applied Biocatalysis: From Fundamental Science to Industrial Applications

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Utilisation of glycogen, ATP and arginine phosphate in exercise and recovery in terrestrial red crabs, Gecarcoidea natalis

Cited by 32 publications

References 37 publications

The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster,Nephrops norvegicus(Linnaeus, 1758)

The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster,Nephrops norvegicus(Linnaeus, 1758)

Lactate accumulation in the intertidal hermit crab, <i>Pagurus samuelis</i>, in response to burial-induced hypoxia

Biocatalytic Phosphorylation of Metabolites

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