Utility of cell-permeable peptides for enhancement of virus-mediated gene transfer to human tumor cells

Lehmusvaara, Saara; Rautsi, Outi; Hakkarainen, Tanja; Wahlfors, Jarmo

doi:10.2144/000112152

Cited by 12 publications

(16 citation statements)

References 10 publications

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“…These differences in the level of cell transduction can be attributed to several factors, including the cell type, method of complex formation, MOI and virus purity. These groups reported also that Tat is sometimes as effective as Pen (Gratton, Yu et al 2003;Lehmusvaara, Rautsi et al 2006).…”

Section: Dicussionsmentioning

confidence: 97%

“…In this study, the transduction efficiency of the complex increased by orders of magnitude for all CPP/Ad complexes up to a CPP concentration of 6.25 µg/10 6 Ad and increased thereafter by only a moderate amount. (Lehmusvaara, Rautsi et al 2006). These differences in the level of cell transduction can be attributed to several factors, including the cell type, method of complex formation, MOI and virus purity.…”

Section: Dicussionsmentioning

confidence: 99%

“…Forming complexes between CPPs and Ad may be achieved through either covalent or noncovalent attachment of CPPs to the Ad capsid. In fact, Gratton et al (Gratton, Yu et al 2003) and Lehmusvaara et al (Lehmusvaara, Rautsi et al 2006) (Gratton, Yu et al 2003;Lehmusvaara, Rautsi et al 2006). The other two CPPs are a polyarginine(pArg), with nine argine residues and Pep1.…”

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confidence: 99%

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Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Nigatu

Vupputuri

Flynn

et al. 2013

Journal of Pharmaceutical Sciences

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Linear low‐density polyethylene (LLDPE), based on butene‐1 or hexene‐1, was irradiated with γ‐rays under vacuum or in the presence of air. The study focused on the influence of the dose rate and the γ‐dose on the thermal properties of LLDPE. Differential scanning calorimetry, thermogravimetric analysis (TGA), and TGA/FTIR techniques were used to address the thermal behavior as a result of γ‐irradiation. During this irradiation, competition between crosslinking and scission reactions, subsequent to oxidation reactions, occurred in the polymeric material, which strongly depends on the experimental conditions. A decrease of the crystallinity for γ‐irradiated samples was observed in particular for samples irradiated under vacuum. This observation may be explained by increased hindrance of segment mobility due to crosslinking reactions that prevent crystal growth. TGA investigations revealed an enhancement of the thermal stability for samples irradiated under vacuum but not for those irradiated in air. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 2790–2795, 2006

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Section: Dicussionsmentioning

confidence: 97%

Section: Dicussionsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Nigatu

Vupputuri

Flynn

et al. 2013

Journal of Pharmaceutical Sciences

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“…CPPs have been shown to transport biomolecules including plasmid DNA, oligonucleotides, siRNA, and proteins with no obvious limitation in the type of cargo [11][12][13]. In fact, Ad has also been delivered into cells using CPPs such as Tat [14,15], Penetratin (Pen) [15][16][17], polyarginine (pArg) [15,18] and Pep1 [15,19].…”

Section: Introductionmentioning

confidence: 99%

Effects of cell-penetrating peptides on transduction efficiency of PEGylated adenovirus

Nigatu

Vupputuri

Flynn

et al. 2015

Biomedicine & Pharmacotherapy

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“…In most of the studies, TAT peptide has been utilized to cargo different proteins and peptides mostly in cultured cells but also in animal models (reviewed extensively by Dietz and Ba¨hr). 7 In the field of gene therapy, TAT peptide has been harnessed to, for example, enhance viral vector transduction efficiency 8,9 or gene-transfer efficiency of liposomes. 10 The mechanism of the TAT peptide internalization is intensively studied, but the results are not easy to interpret and the exact route is still arguable.…”

Section: Introductionmentioning

confidence: 99%

Characterization of HIV-1 TAT peptide as an enhancer of HSV-TK/GCV cancer gene therapy

et al. 2008

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Cancer suicide gene therapy based on herpes simplex virus type I thymidine kinase (HSV-TK) and ganciclovir (GCV) suffers from the lack of efficacy in clinical use, which is mostly due to low gene-transfer efficiency and absence of bystander effect in tumors. We have previously demonstrated the enhancement of GCV cytotoxicity by fusing the HSV-TK with the cell penetrating peptide from HIV-1 transactivator protein transduction domain (TAT PTD). Despite the earlier promising results, we found that the triple fusion protein HIV-1 transactivator protein transduction domain-thymidine kinase suicide gene-green fluorescent protein marker gene (TAT-TK-GFP) increased GCV cytotoxicity only in 3/12 of different human tumor cell lines. Extended GCV exposure enhanced the cytotoxic effect of HSV-TK/GCV gene therapy, but the difference between TK-GFP and TAT-TK-GFP was not statistically significant. The modest improvement on cell killing mediated by TAT PTD in Chinese hamster ovary cells appeared to be associated with cellsurface heparan sulfate proteoglycan (HSPG) composition. However, TAT-mediated increased cell death did not correlate with the density of cell-surface HSPG expression in different tumor cell lines. In conclusion, although some degree of enhancement by TAT was shown in certain tumor cells in vitro, it is unlikely that TAT peptide linked to a suicide protein could be a useful booster of in vivo gene therapy trials.

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Utility of cell-permeable peptides for enhancement of virus-mediated gene transfer to human tumor cells

Cited by 12 publications

References 10 publications

Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Effects of cell-penetrating peptides on transduction efficiency of PEGylated adenovirus

Characterization of HIV-1 TAT peptide as an enhancer of HSV-TK/GCV cancer gene therapy

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