Utility of ITS1–5.8S–ITS2 and 16S mitochondrial DNA sequences for species identification and phylogenetic inference within the Rhinonyssus coniventris species complex (Acari: Rhinonyssidae)

Rojas, Manuel de; Úbeda, J. M.; Cutillas, Cristina; Mora, María Dolores González; Ariza, C.; Guevara, Diego

doi:10.1007/s00436-006-0356-z

Cited by 22 publications

(16 citation statements)

References 20 publications

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“…However, in spite of the utility of 16S rDNA to solve taxonomic and phylogenetic questions between closely related species, such as those included in Rhinonyssus coniventris complex (de Rojas et al 2007), our results show that 16S mitochondrial rDNA does not seem to be a good marker for comparisons at the population level in this group of mites.…”

Section: Discussioncontrasting

confidence: 76%

Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans

et al. 2011

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A morphobiometrical and molecular study of two populations of Demodex folliculorum from humans isolated from different habitats, skin and eyelashes follicles, were carried out. Morphological and biometrical studies revealed two closely related populations with any distinctive characteristics. For molecular study, a 436-bp region of the 16S rDNA gene and a 453-bp region of the cytochrome oxidase I (COI) gene from individual mites of each population considered were sequenced. Intraindividual and interindividual sequence variation was studied in both populations. Our data show that 16S rDNA is not a useful marker to discriminate between populations; however, COI gene sequences can help to identify the two populations considered, which are morphologically very close and difficult to separate by classic methods. These results are in agreement with the morphological and biometrical differences detected between D. folliculorum from eyelashes and human skin. This study appeals for the revision of the taxonomic status of the D. folliculorum populations, as well as for the species included within genus Demodex.

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Section: Discussioncontrasting

confidence: 76%

Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans

et al. 2011

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“…The entire ITS fragment of D. gallinae amplified from each sample using primers ITSDGF and ITSDGR was approximately 500 bp in length (Fig. 2), as reported by de Rojas et al (2007). In no case was product amplified from no-DNA sample.…”

Section: Resultsmentioning

confidence: 64%

“…Furthermore, detailed morphological descriptions of the immature stages remain unknown for most tick species. To circumvent somehow these kinds of difficulties, several approaches using different genetic makers have been evaluated for the identification and phylogenetic studies of ticks (Wesson et al 1993;McLain et al 1995;Zahler and Gothe 1997;Poucher et al 1999;Fukunaga et al 2000;Hlinka et al 2002;Shaw et al 2002;Rees et al 2003;de Rojas et al 2007;Marrelli et al 2007). Concerning the public health aspects, molecular methods applied to the diagnosis of ticks can be useful and practical.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA

Chitimia

Lin

Cosoroabå³

et al. 2009

Parasitol Res

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In the present study, four hard tick species and one soft tick species, namely, Dermacentor marginatus, Haemaphysalis punctata, Haemaphysalis parva, Ixodes ricinus, and Dermanyssus gallinae, from south-western Romania were characterized genetically by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA), using a hard tick, Haemaphysalis longicornis, from China for comparative purposes. The ITS rDNA was amplified by polymerase chain reaction (PCR) and sequenced from individual ticks. The lengths of the ITS-1 sequences were 238-1819 bp, and the lengths of ITS-2 were 137-1695 bp, respectively, for all ticks sequenced. While sequence variation within a hard tick species was 0-1.5%, nucleotide differences between hard tick species ranged 2-25.2%, indicating that ITS rDNA sequences provide genetic markers for the differentiation of hard ticks from Romania. Hence, a PCR-linked restriction fragment length polymorphism approach was developed for their unequivocal differentiation based on ITS-1 rDNA. This is the first characterization of ticks from Romania using a genetic approach, which provides the foundation for further studies on ticks in Romania and has implications for studying the population genetic structure of the Romanian ticks and for identification and differentiation of closely related ticks.

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“…Unlike the rRNA genes which are highly conserved between closely related species, the spacer regions (ITS1 and ITS2) are subject to higher evolutionary rates and are therefore more variable in their lengths and nucleotide composition (Hillis and Dixon, 1991). These regions have therefore been used for the discrimination of closely related species and subspecies, and in the description of new species (Zahler et al, 1998;Holman et al, 2003;Lew et al, 2003;Aktas et al, 2007;de Rojas et al, 2007;Hilpertshauser et al, 2007;Saito-Ito et al, 2008;Niu et al, 2009;Bosman et al, 2010).…”

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confidence: 99%

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Chaisi

Collins

Oosthuizen

2014

Veterinary Parasitology

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Theileria buffeli/T. sergenti/T. orientalis is a group of benign and mildly pathogenic species of cattle and buffalo. In a previous study, we identified T .buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe-iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterize the 18S rRNA gene and complete internal transcribed spacer (ITS1-5.8S-ITS2) region of T. buffeli samples, and to establish the phylogenetic position of this species based on these loci. The 18S rRNA gene and the complete ITS region were amplified from DNA extracted from blood samples originating from buffalo in these localities. The PCR products were cloned and the resulting recombinants sequenced. We identified novel T. buffeli 18S rRNA gene and ITS genotypes from buffalo in the AEGP, and novel T. sinensis 18S rRNA genotypes from buffalo in the HIP. Phylogenetic analyses indicated that the T. buffeli-like sequences were similar to T. buffeli sequences from cattle and buffalo in China and India, and the T. sinensis-like sequences were similar to T. sinensis 18S rRNA sequences of cattle and yak in China. There was extensive sequence variation between the novel T.1 buffeli genotypes of the African buffalo and previously described T. buffeli and T. sinensis genotypes. The presence of organisms with T. buffeli-like and T. sinensis-like genotypes in the African buffalo could be of significant importance, particularly to the cattle industry in South Africa as these animals might act as sources of infections to naïve cattle. This is the first report on the characterization of the full-length 18S rRNA gene and ITS region of T. buffeli and T. sinensis genotypes in South Africa. Our study provides invaluable information towards the classification of this complex group of benign and mildly pathogenic species.

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Utility of ITS1–5.8S–ITS2 and 16S mitochondrial DNA sequences for species identification and phylogenetic inference within the Rhinonyssus coniventris species complex (Acari: Rhinonyssidae)

Cited by 22 publications

References 20 publications

Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans

Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans

Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

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