Utility of real-time polymerase chain reaction for the assessment of CDKN2A homozygous deletion in adult-type IDH-mutant astrocytoma

Shimizu, Yuzaburo; Suzuki, Mario; Akiyama, Osamu; Ogino, Ikuko; Matsushita, Yuko; Satomi, Kaishi; Yanagisawa, Shunsuke; Ohno, Makoto; Takahashi, Manabu; Miyakita, Yasuji; Narita, Yoshitaka; Ichimura, Koichi; Kondo, Akihide

doi:10.1007/s10014-023-00450-z

Brain Tumor Pathol

2023

DOI: 10.1007/s10014-023-00450-z

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Utility of real-time polymerase chain reaction for the assessment of CDKN2A homozygous deletion in adult-type IDH-mutant astrocytoma

Yuzaburo Shimizu

Mario Suzuki²,

Osamu Akiyama³

et al.

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2024

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Cited by 4 publications

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Loss of methylthioadenosine phosphorylase immunoreactivity correlates with poor prognosis and elevated uptake of 11C-methionine in IDH-mutant astrocytoma

Yamamura,

Tamura,

Kobayashi

et al. 2024

J Neurooncol

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Loss of methylthioadenosine phosphorylase immunoreactivity correlates with poor prognosis and elevated uptake of 11C-methionine in IDH-mutant astrocytoma

Yamamura,

Tamura,

Kobayashi

et al. 2024

J Neurooncol

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Patient-derived glioma organoids real time identification of IDH mutation, 1p/19q-codeletion and CDKN2A/B homozygous deletion with differential ion mobility spectrometry

Hermelo,

Haapala,

Mäkelä

et al. 2024

J Neurooncol

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Purpose Extent of brain tumor resection continues to be one of the central decisions taken during standard of care in glioma patients. Here, we aimed to evaluate the most essential molecular factors, such as IDH (isocitrate dehydrogenase) mutation in gliomas classification with patient-derived glioma organoids (PGOs) using differential mobility spectrometry (DMS). Methods we prospectively recruited 12 glioma patients, 6 IDH-mutated and 6 IDH wild-type tumors, from which PGOs were generated ex-vivo. Altogether, 320 PGOs DMS spectra were analyzed with a classifier algorithm based on linear discriminant analysis (LDA). Results LDA model classification accuracy (CA) obtained between IDH-mutant and IDH wild-type PGOs was 90% (91% sensitivity and 89% specificity). Furthermore, 1p/19q codeletion classification within IDH mutant PGOs reached 98% CA (93% sensitivity and 99% specificity), while CDKN2A/B homozygous loss status had 86% CA (63% sensitivity 93% specificity). Conclusion DMS suitability to differentiate IDH-mutated PGOs was thus validated in ex vivo cultured samples, PGOs. Preliminary results regarding 1p/19q codeleted PGOs and CDKN2A/B loss PGOs identification endorse testing in a prospective intraoperative glioma patient cohort. Our results reveal a sample classification set-up that is compatible with real-time intraoperative surgery guidance.

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Intraoperative Real-Time IDH Diagnosis for Glioma Based on Automatic Analysis of Contrast-Enhanced Ultrasound Video

Xie,

Zhao,

Zhang

et al. 2024

Ultrasound in Medicine & Biology

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Utility of real-time polymerase chain reaction for the assessment of CDKN2A homozygous deletion in adult-type IDH-mutant astrocytoma

Cited by 4 publications

References 22 publications

Loss of methylthioadenosine phosphorylase immunoreactivity correlates with poor prognosis and elevated uptake of 11C-methionine in IDH-mutant astrocytoma

Loss of methylthioadenosine phosphorylase immunoreactivity correlates with poor prognosis and elevated uptake of 11C-methionine in IDH-mutant astrocytoma

Patient-derived glioma organoids real time identification of IDH mutation, 1p/19q-codeletion and CDKN2A/B homozygous deletion with differential ion mobility spectrometry

Intraoperative Real-Time IDH Diagnosis for Glioma Based on Automatic Analysis of Contrast-Enhanced Ultrasound Video

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