Utility of some nitrogen-fixing microorganisms in the phyllosphere of crop plants

Nandi, A. S.; Sen, S. P.

doi:10.1007/bf02370046

Cited by 19 publications

(7 citation statements)

References 9 publications

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“…The bacterial diversity associated with rhizosphere of wheat as observed in this study was significantly higher as compared to the previous reports (Guemouri-Athmani et al Requena et al 1997;Pedersen et al 1978). Streptomyces venezualae, one of the actinomycetes isolated in the present study was also a reported nitrogen fixer (Nandi and Sen 1981). Interestingly, the symbiotic nitrogen fixers such as Ensifer sp.…”

Section: Resultscontrasting

confidence: 67%

Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

2009

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Microbial biota associated with wheat rhizosphere during flowering-stage and their plant growth promoting traits was investigated. 16S rRNA gene sequencing was performed of the isolates, which were obtained on selective media. Isolates belonged to: alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria; Actinobacteria; Bacteroidetes and Firmicutes. Bacillus was the most dominant genus (34.7%) followed by Pseudomonas (14.4%). Among diazotrophs, Arthrobacter sp. (n=3), Cupriavidus sp. (n=3) and Stenotrophomonas sp. (n=4) occurred more frequently. Most of the isolates produced indole acetic acid. Ac. baumannii , Ps. lini, Ser. marcescens, C. respiraculi and Ag.tumfaciens solubilized phosphate. Two Acinetobacter strains, five Pseudomonas srains and four Bacillus strains produced siderophore. Strains of Ps. aeruginosa, Ps. lini and Ps. thivervalensis exhibited in vitro fungal growth inhibition. Arthrobacter globiformis Y2S3 exhibited indole acetic acid production, siderophore production and antifungal activity. Plant growth promoting traits of these rhizobacteria indicated beneficial relationship between rhizobacteria and wheat plants. Several of the strains could be further developed as an effective bio-inoculant. Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

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Section: Resultscontrasting

confidence: 67%

Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

2009

IJMB

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“…Bacteria contribute significantly to pathogenic control, N 2 fixation, nutrient cycling, and decomposition in phyllosohere ecosystems (Ruinen, 1956;Nandi and Sen, 1981;Berg et al, 2005;Compant et al, 2005). Any effects of fungicides on microbial communities may have subsequent impacts on high trophic levels.…”

Section: Introductionmentioning

confidence: 99%

Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere

Bai

Jin

et al. 2010

Journal of Environmental Sciences

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Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application.

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“…The necessity of a selective medium and selective growth conditions probably account for the appearance of only a single report for the isolation of Xanthobacter species from plant roots. Nandi and Sen (1981) reported that rice yield was doubled when Mycobacterium flavum (now Xanthobacter flavus) was sprayed onto rice leaves. In an ~SN study of soil diazotrophs, rice plants, grown in sterile soil amended with lactose, received 18% of the N 2 fixed by Xanthobacter flavus (Kalininskaya et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Effect of Xanthobacter, isolated and characterized from rice roots, on growth of wetland rice

1991

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With an autotrophic, N-flee medium, Xanthobacter populations were isolated from the roots of wetland rice grown under field conditions. Xanthobacter populations ranged from 3.2 x 10 4 to 5.1 × 10 5 colony-forming units (cfu) g-~ of root and averaged 47-fold higher on the root or rhizoplane than in the neighbouring nonrhizosphere. Characterization studies indicated dissimilarities in carbon utilization and motility among the isolated Xanthobacter strains and other recognized Xanthobacter species. Under gnotobiotic conditions, the population of one isolate, Xanthobacter sp. JW-KR1, increased from 105 to 107 cfu plant-l 1 d after inoculation when a rice plant was present, but declined to numbers below the limit of detection (<104cfu assembly -l) after 3d in the absence of a plant. Scanning electron microscopy revealed Xanthobacter as pleomorphic forms on the rhizoplane. To assess the effect of Xanthobacter on plant growth, rice plants were grown under greenhouse conditions in plant assemblies containing sand and half-strength Hoagland's nutrient solution with and without nitrogen. Plants were either inoculated with 105 cfu Xanthobacter g-t of sand or left uninoculated. After 40 d, plants without nitrogen showed no significant differences in top or root dry weight, plant height, root length, or number of tillers or leaves, whether the plants were inoculated or uninoculated. However, when nitrogen was added, inoculated plants had a significantly larger top dry weight (15%) and number of leaves (19%) than uninoculated plants. Under conditions of added and no added nitrogen, acetylene reduction assays showed Xanthobacter sp. JW-KR1 produced <0.1 (below detection limit) and 7 nmol C2H 4 plant-~ h-1, respectively. Under the conditions studied, the results suggest that both Xanthobacter and wetland rice derive some benefits from their association.

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Utility of some nitrogen-fixing microorganisms in the phyllosphere of crop plants

Cited by 19 publications

References 9 publications

Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere

Effect of Xanthobacter, isolated and characterized from rice roots, on growth of wetland rice

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