1999
DOI: 10.1590/s0100-06831999000400026
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Utilização de microondas na avaliação da biomassa microbiana do solo

Abstract: RESUMOO presente trabalho teve por objetivo determinar o carbono e o nitrogênio presentes na biomassa microbiana, usando a irradiação do forno de microondas em substituição ao clorofórmio nos métodos fumigação-incubação e fumigação-extração. Foram utilizadas duas amostras de um Podzólico Vermelho-Escuro, submetidas aos procedimentos de incubação e de extração, após serem fumigadas com clorofórmio e irradiadas com microondas por diferentes períodos (2, 5, 10, 15, 20, 30, 40 e 50 min). Observou-se que a irradiaç… Show more

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Cited by 106 publications
(71 citation statements)
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“…Subsequently, the MB-C was analyzed in triplicate, subdivided into six subsamples of 30 g, of which three were subjected to radiation in a microwave oven to cause cell lysis. The samples were subjected to microwave radiation for 2 min at 1.62x10 5 J (Ferreira et al, 1999 . The coefficient (Kc) used in the calculation to determine the MB-C was 0.33.…”
Section: Methodsmentioning
confidence: 99%
“…Subsequently, the MB-C was analyzed in triplicate, subdivided into six subsamples of 30 g, of which three were subjected to radiation in a microwave oven to cause cell lysis. The samples were subjected to microwave radiation for 2 min at 1.62x10 5 J (Ferreira et al, 1999 . The coefficient (Kc) used in the calculation to determine the MB-C was 0.33.…”
Section: Methodsmentioning
confidence: 99%
“…The MBC was determined according to the methodology described by Vance et al (1987), in which chloroform is replaced by a microwave oven (for 4 min) to eliminate microorganisms and release cell components, as suggested by Ferreira et al (1999). For the determination of size distribution of water aggregate stability (mean weighted diameter MWD) a 5 x 5 x 20 cm soil cube was collected (care should be taken to avoid segmentation of the sample in the field) and the sample was identified in the laboratory according to a methodology described by Kemper & Rosenau (1986), calculated as described by Palmeira et al (1999).…”
Section: Methodsmentioning
confidence: 99%
“…O C da biomassa foi determinado segundo Ferreira et al (1999), empregando-se o método da irradiação/ incubação, que apresenta como princípio básico a eliminação de microrganismos por meio da irradiação eletromagnética de forno de microondas, antes da avaliação da quantidade de CO 2 liberada após incubação por 10 dias, 25 °C. A atividade respiratória da biomassa microbiana, ou respiração basal do solo, foi avaliada pela quantificação do CO 2 liberado durante a incubação do solo em sistema fechado, onde o CO 2 foi capturado em solução de NaOH 1 mol L -1 e posteriormente titulada com HCl 1 mol L -1 , segundo método adaptado de Anderson (1982).…”
Section: Avaliação Dos Atributos Microbianos Do Solounclassified