Utilization and transport of l-arabinose by non-Saccharomyces yeasts

Knoshaug, Eric P.; Franden, Mary Ann; Stambuk, Boris U.; Zhang, Min; Singh, Arjun

doi:10.1007/s10570-009-9319-8

Cited by 24 publications

(22 citation statements)

References 27 publications

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“…and Pichia sp., Arxula adeninivorans, Debaryomyces hansenii and K. marxianus have been characterized for their ability for arabinose uptake (Lucas and van Uden, 1986;Fonseca et al, 2007;Knoshaug et al, 2009). The kinetics of arabinose uptake revealed the presence of at least two kinds of transport systems, a low affinity, high capacity facilitated diffusion component and a high affinity, low capacity proton symporter which requires one proton to be co-transported with each molecule of arabinose transported Knoshaug et al, 2009). The genetic sequence of the arabinose transporters from the yeasts C. arabinofermentans and P. stipitis have been elucidated and the genes functionally expressed in S. cerevisiae (Boles and Keller, 2008;Fonseca et al, 2009).…”

Section: Strainmentioning

confidence: 99%

Bioconversion of lignocellulose-derived sugars to ethanol by engineeredSaccharomyces cerevisiae

Madhavan

Srivastava

Kondo

et al. 2011

Critical Reviews in Biotechnology

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Lignocellulosic biomass from agricultural and agro-industrial residues represents one of the most important renewable resources that can be utilized for the biological production of ethanol. The yeast Saccharomyces cerevisiae is widely used for the commercial production of bioethanol from sucrose or starch-derived glucose. While glucose and other hexose sugars like galactose and mannose can be fermented to ethanol by S. cerevisiae, the major pentose sugars D-xylose and L-arabinose remain unutilized. Nevertheless, D-xylulose, the keto isomer of xylose, can be fermented slowly by the yeast and thus, the incorporation of functional routes for the conversion of xylose and arabinose to xylulose or xylulose-5-phosphate in Saccharomyces cerevisiae can help to improve the ethanol productivity and make the fermentation process more cost-effective. Other crucial bottlenecks in pentose fermentation include low activity of the pentose phosphate pathway enzymes and competitive inhibition of xylose and arabinose transport into the cell cytoplasm by glucose and other hexose sugars. Along with a brief introduction of the pretreatment of lignocellulose and detoxification of the hydrolysate, this review provides an updated overview of (a) the key steps involved in the uptake and metabolism of the hexose sugars: glucose, galactose, and mannose, together with the pentose sugars: xylose and arabinose, (b) various factors that play a major role in the efficient fermentation of pentose sugars along with hexose sugars, and (c) the approaches used to overcome the metabolic constraints in the production of bioethanol from lignocellulose-derived sugars by developing recombinant S. cerevisiae strains.

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Section: Strainmentioning

confidence: 99%

Bioconversion of lignocellulose-derived sugars to ethanol by engineeredSaccharomyces cerevisiae

Madhavan

Srivastava

Kondo

et al. 2011

Critical Reviews in Biotechnology

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“…Although functional expression of arabinose transporters in S. cerevisiae has not been reported so far, some entries can be found in public databases concerning arabinose transport in fungi, mostly related to patent applications: Ambrosiozyma monospora LAT1 and LAT2 (EMBL AY923868 and AY923869, respectively, R. Verho et al ), C. arabinofermentans ART1 (Fonseca et al , 2007a), K. marxianus KmLAT1 and P. guilliermondii PgLAT2 (Knoshaug et al , 2007) and P. stipitis araT (Boles & Keller, 2008).…”

Section: Pentose Transportmentioning

confidence: 99%

Hexose and pentose transport in ascomycetous yeasts: an overview

Leandro

Fonseca

Gonçalves

2009

FEMS Yeast Research

130

106

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The biochemical characterization of sugar uptake in yeasts started five decades ago and led to the early production of abundant kinetic and mechanistic data. However, the first accurate overview of the underlying sugar transporter genes was obtained relatively late, due mainly to the genetic complexity of hexose uptake in the model yeast Saccharomyces cerevisiae. The genomic era generated in turn a massive amount of information, allowing the identification of a multitude of putative sugar transporter and sensor-encoding genes in yeast genomes, many of which are phylogenetically related. This review aims to briefly summarize our current knowledge on the biochemical and molecular features of the transporters of hexoses and pentoses in yeasts, when possible establishing links between previous kinetic studies and genomic data currently available. Emphasis is given to recent developments concerning the identification of d-xylose and l-arabinose transporter genes, which are thought to be key players in the optimization of S. cerevisiae strains for bioethanol production from lignocellulose hydrolysates.

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“…Among these yeasts, S. stipitis is commonly reported as the best native xylose-fermenting yeast in terms of product yield and productivity. Although many yeasts can assimilate L-arabinose [15,22], only a few have been reported to ferment L-arabinose to ethanol [6,16]. The highest ethanol yield has been reported for Candida arabinofermentans, but it only produced 1.9-3.4 g l -1 ethanol from 80-100 g l -1 L-arabinose in 12-14 days [16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Candida sp. NY7122, a novel pentose-fermenting soil yeast

Watanabe

Andõ

Nakamura

2012

Journal of Industrial Microbiology and Biotechnology

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Yeasts that ferment both hexose and pentose are important for cost-effective ethanol production. We found that the soil yeast strain NY7122 isolated from a blueberry field in Tsukuba (East Japan) could ferment both hexose and pentose (D-xylose and L-arabinose). NY7122 was closely related to Candida subhashii on the basis of the results of molecular identification using the sequence in the D1/D2 domains of 26S rDNA and 5.8S-internal transcribed spacer region. NY7122 produced at least 7.40 and 3.86 g l⁻¹ ethanol from 20 g l⁻¹ D-xylose and L-arabinose within 24 h. NY7122 could produce ethanol from pentose and hexose sugars at 37°C. The highest ethanol productivity of NY7122 was achieved under a low pH condition (pH 3.5). Fermentation of mixed sugars (50 g l⁻¹ glucose, 20 g l⁻¹ D-xylose, and 10 g l⁻¹ L-arabinose) resulted in a maximum ethanol concentration of 27.3 g l⁻¹ for the NY7122 strain versus 25.1 g l⁻¹ for Scheffersomyces stipitis. This is the first study to report that Candida sp. NY7122 from a soil environment could produce ethanol from both D-xylose and L-arabinose.

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Utilization and transport of l-arabinose by non-Saccharomyces yeasts

Cited by 24 publications

References 27 publications

Bioconversion of lignocellulose-derived sugars to ethanol by engineeredSaccharomyces cerevisiae

Bioconversion of lignocellulose-derived sugars to ethanol by engineeredSaccharomyces cerevisiae

Hexose and pentose transport in ascomycetous yeasts: an overview

Characterization of Candida sp. NY7122, a novel pentose-fermenting soil yeast

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