UTILIZATION OF AGROINDUSTRIAL BY-PRODUCTS AS SUBSTRATE IN ENDOGLUCANASE PRODUCTION BY Streptomyces diastaticus PA-01 UNDER SUBMERGED FERMENTATION

Bispo, A. S. R.; Andrade, Jackeline Pereira; Souza, Danilo Tosta; Teles, Zozilene Nascimento Santos; Nascimento, Rodrigo Pires do

doi:10.1590/0104-6632.20180352s20160415

Cited by 12 publications

(6 citation statements)

References 33 publications

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“…Highest cellulase production from S.cerevisiae on pineapple peel substrate was achieved on day 3 [67]. In addition, the endoglucanase synthesis from Streptomyces diastaticus was investigated using a mix of sugarcane bagasse, oat bran, and corn steep liquor, reporting the highest level after day 5, and a good level on day 4 [68].…”

Section: Resultsmentioning

confidence: 99%

Solid-State Yeast Fermented Wheat and Oat Bran as A Route for Delivery of Antioxidants

2019

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The purpose of our study was to evaluate the potential of solid-state yeast fermentation (SSYF) in improving the phenolic acid content and composition, and the antioxidant activity of commercial wheat bran (WB) and oat bran (OB). The ultrasound-assisted methanolic extracts were compared for their total phenolic content (TPC), phenolics composition, and in vitro antioxidant activity in order to study the effect of fermentation time on the chemical profile and activity of bioactive compounds. The comparative analysis revealed significant differences (p < 0.05) between days of fermentation (0 through 6). The highest TPCs were obtained on day 3 for WB (0.84 ± 0.05 mg of gallic acid equivalents [GAE]/g dry weight [DW]), and on day 4 for OB (0.45 ± 0.02 mg GAE/g DW). The highest relative percentage increase in the phenolics concentration of WB was also registered on day 3 (ferulic acid +56.6%, vanillic acid +259.3%, dihydroxybenzoic acids +161.2%, apigenin-glucoside +15.3%); for OB, this was observed on day 4 (avenanthramide 2f +48.5%, ferulic acid +21.2%). Enhanced antioxidant activities were significantly correlated with the highest TPCs. Our results suggest that SSYF may be a useful procedure for enrichment of antioxidants in cereal bran, considering the design of different functional foods and nutraceuticals.

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Section: Resultsmentioning

confidence: 99%

Solid-State Yeast Fermented Wheat and Oat Bran as A Route for Delivery of Antioxidants

2019

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“…The lytic enzymes isolated from bacteria are also important having wide variety of applications in textile, beverages, and pharmaceutical industries because of their catalytic activity to convert complex compounds into useful by-products [8]. Different microbial species such as B. subtilis [9], Penicillium [10], Aspergillus [11] and Thermomonospora [12] have been reported to produce cellulase and chitinase enzymes. Cellulose is the most abundant polysaccharide and the most predominant renewable agricultural waste in the world [13].…”

Section: Introductionmentioning

confidence: 99%

Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11

et al. 2023

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Cellulose and chitin are the most abundant naturally occurring biopolymers synthesized in plants and animals and are used for synthesis of different organic compounds and acids in the industry. Therefore, cellulases and chitinases are important for their multiple uses in industry and biotechnology. Moreover, chitinases have a role in the biological control of phytopathogens. A bacterial strain Bacillus subtilis TD11 was previously isolated and characterized as a putative biocontrol agent owing to its significant antifungal potential. In this study, cellulase and chitinase produced by the strain B. subtilis TD11 were purified and characterized. The activity of the cellulases and chitinases were optimized at different pH (2 to 10) and temperatures (20 to 90°C). The substrate specificity of cellulases was evaluated using different substances including carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), and crystalline substrates. The cellulase produced by B. subtilis TD11 had a molecular mass of 45 kDa while that of chitinase was 55 kDa. The optimal activities of the enzymes were found at neutral pH (6.0 to 7.0). The optimum temperature for the purified cellulases was in the range of 50 to 70°C while, purified chitinases were optimally active at 50°C. The highest substrate specificity of the purified cellulase was found for CMC (100%) followed by HEC (>50% activity) while no hydrolysis was observed against the crystalline substrates. Moreover, it was observed that the purified chitinase was inhibitory against the fungi containing chitin in their hyphal walls i.e., Rhizoctonia, Colletotrichum, Aspergillus and Fusarium having a dose-effect relationship.

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“…Streptomyces diastaticus được phân lập trên Cua Đốm đỏ Portunus sanguinolentus với hoạt tính kháng màng biofilm có thể đối kháng mạnh mẽ với nấm Candida albibcans (Siddharthan et al, 2020). Streptomyces diastaticus cũng được phân lập trong hang đá ở Brazil làm chủng xạ khuẩn sinh enzyme endoglucanase ứng dụng trong sản xuất enzyme công nghiệp và sản xuất ethanol của quốc gia này (Bispo et al, 2018).…”

Section: địNh Danh Các Chủng Xạ Khuẩn Mục Tiêuunclassified

Phân Lập Và Tuyển Chọn Các Chủng Xạ Khuẩn Có Khả Năng Sinh Enzyme Ngoại Bào, Đối Kháng Vibrio Spp.

Phúc¹,

Hương²,

Đan³

et al. 2021

Tạp chí Khoa học

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Nghiên cứu được tiến hành nhằm phân lập và tuyển chọn các chủng xạ khuẩn từ các ao nuôi tôm thẻ chân trắng ở huyện Thạnh Phú, tỉnh Bến Tre có khả năng sinh enzyme ngoại bào và đối kháng với Vibrio spp. Có tất cả 26 chủng xạ khuẩn được phân lập, trong đó 5 chủng TM1, TM2, TM7, TM21 và TM22 được xác định đều là các chủng đa chức năng. Cả 5 chủng này đều có khả năng sản sinh tốt cả 3 loại enzyme protease, amylase và cellulase. Đặc biệt, 3 chủng TM1, TM2 và TM21 còn có khả năng đối kháng với Vibrio spp.. Phân tích kết quả giải trình tự gen 16S rRNA cho thấy cả 3 chủng TM1, TM2, TM21 đều thuộc loài Streptomyces hygroscopicus. Hai chủng TM7 và TM22 được xác định lần lượt là Streptomyces diastaticus và Streptomyces spiralis.

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UTILIZATION OF AGROINDUSTRIAL BY-PRODUCTS AS SUBSTRATE IN ENDOGLUCANASE PRODUCTION BY Streptomyces diastaticus PA-01 UNDER SUBMERGED FERMENTATION

Cited by 12 publications

References 33 publications

Solid-State Yeast Fermented Wheat and Oat Bran as A Route for Delivery of Antioxidants

Solid-State Yeast Fermented Wheat and Oat Bran as A Route for Delivery of Antioxidants

Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11

Phân Lập Và Tuyển Chọn Các Chủng Xạ Khuẩn Có Khả Năng Sinh Enzyme Ngoại Bào, Đối Kháng Vibrio Spp.

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