Utilization of an evaporative light scattering detector for high-performance size-exclusion chromatography of galacturonic acid oligomers

Cameron, Randall G.; Hotchkiss, Arland T.; Kauffman, Steven W.; Grohmann, K.

doi:10.1016/s0021-9673(03)01171-3

Cited by 9 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPAEC–ELSD provides high resolution and sensitive detection of oligogalacturonates up to an approximate DP equal to 70 , . We applied this method to the separation and detection of demethylesterified oligogalacturonates in extracts and precipitates from steam treated CPW.…”

Section: Resultsmentioning

confidence: 99%

“…Extracts were injected and eluted with a 0–1 mol L −1 ammonium formate gradient (0–60 min, increasing from 0 to 60%, 60–180 min, from 61 to 80%, 180–210 min 100%) in deionized water with a flow rate of 1 mL min −1 . The DP of oligogalacturonates was identified by comparing elution profiles with elution times of previously run digests of polygalacturonic acid (PGA) and co–elution with known standards , …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Extraction and recovery of pectic fragments from citrus processing waste for co–production with ethanol

Grohman

Cameron

Kim

et al. 2012

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Approximately 0.7 − 1 × 10 6 dry tons of citrus processing waste (CPW) are produced annually in the United States. CPW is sold as animal feed but often the financial return does not exceed the production cost. Polysaccharides comprise 40% of the total dry matter of which pectin is the major component. CPW was steam treated to extract pectin fragments (PFs) as a value added co-product prior to fermentation of other sugars for biofuels production.RESULTS: PFs were extracted in high yields, along with polymeric arabinans, galactans and arabinogalactans. The extracted polysaccharides ranged in size from small oligomers to polymers of ∼ 700 000 g mol −1 . Acidified treatments led to greater fragmentation of water soluble polysaccharides, but did not enhance fragmentation of pectins to small oligomers (> 30 mer). Methylesterified PFs, arabinans and galactans were recovered by ethanol precipitation while demethylesterified PFs were recovered and purified by precipitation with dilute HCl. CONCLUSION: Steam treatment of CPW provides for rapid, efficient fragmentation of protopectin into highly methylesterifiedPFs that could be recovered by precipitation. The steaming process for preparation of PFs is environmentally friendly. No toxic chemicals are introduced and the remaining CPW can be used in fermentations to produce ethanol and other compounds.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Extraction and recovery of pectic fragments from citrus processing waste for co–production with ethanol

Grohman

Cameron

Kim

et al. 2012

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…The use of salt gradients has been of particular value in studying the fractionation of polysaccharides with ionic functional groups according to charge characteristics, [7,8] and in this application RI is not used as a mass detector. In future work, it may be of value to obtain absolute molecular size data while applying a gradient elution for the study of charged polysaccharides with MALLS.…”

Section: Introductionmentioning

confidence: 99%

Use of an Evaporative Light Scattering Detector Coupled to MALLS for Determination of Polysaccharide Molecular Weights

Luzio

2006

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

A method to calibrate and use an evaporative light scattering detector (ELS) as a mass detector for molecular weight determination of polysaccharides using a multi-angle laser light scattering detector (MALLS) is described. The calibration of the ELS is performed under isocratic conditions using concentration values obtained from an interferometric refractive index detector (RI). The observed response fit a power trendline (y ¼ ax b ) for the concentration response of the RI versus the voltage response of the ELS. The combined average log a coefficient was (5.472, s.d. ¼ 0.020) and the average b coefficient was (1.372, s.d. ¼ 0.004) for all the runs. Band broadening, which could occur between the detectors, was not observed and did not affect the calibration values. Without adjustment the ELS was used as a mass detector for MALLS to accurately determine molecular weights (MW) at elevated buffer concentrations.

show abstract

“…Over the years, many techniques such as high-performance liquid chromatography (HPLC), high-performance anion exchange chromatography (HPAEC), capillary eletrophoresis (CE), and NMR spectroscopy have been used for the analysis of enzymatic or chemical degradation products of pectin polymers [10][11][12][13][14][15][16]. These techniques are very useful for the identiWcation and structure characterization of degraded GalUA oligomers.…”

mentioning

confidence: 99%

Determination of pathogen-related enzyme action by mass spectrometry analysis of pectin breakdown products of plant cell walls

Lurie

Greve

et al. 2005

Analytical Biochemistry

View full text Add to dashboard Cite

Utilization of an evaporative light scattering detector for high-performance size-exclusion chromatography of galacturonic acid oligomers

Cited by 9 publications

References 15 publications

Extraction and recovery of pectic fragments from citrus processing waste for co–production with ethanol

Extraction and recovery of pectic fragments from citrus processing waste for co–production with ethanol

Use of an Evaporative Light Scattering Detector Coupled to MALLS for Determination of Polysaccharide Molecular Weights

Determination of pathogen-related enzyme action by mass spectrometry analysis of pectin breakdown products of plant cell walls

Contact Info

Product

Resources

About