Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation

Acharya, Bhavik K.; Mohana, Sarayu; Jog, Rahul; Divecha, Jyoti; Madamwar, Datta

doi:10.1016/j.jenvman.2010.05.001

Cited by 41 publications

(10 citation statements)

References 29 publications

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“…Bacillus species are attractive industrial organisms because of their high growth rates leading to short fermentation cycle times, capacity to secrete proteins into the extracellular medium and their general environmental safety [23]. However, the yield of target metabolisms depends on the formulation of fermentation medium and the biosynthesis conditions [24,25].…”

Section: Discussionmentioning

confidence: 99%

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Liu

Shen

Yin

2021

Biotechnology & Biotechnological Equipment

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In previous study, we identified a novel protein elicitor AMEP412 from Bacillus subtilis BU412, which could trigger plant defense response and induce systemic acquired resistance against diseases. In this paper, the production of the new elicitor was optimised. First, optimal carbon source and nitrogen source were determined as glucose and yeast extract by single factor test. Then, effects of seven different factors on AMEP412 production was studied by Plackett-Burman design. Significant factors (yeast extract, culture temperature and culture time) were selected for further optimisation using central composite design by response surface methodology. The optimum conditions for AMEP412 production were yeast extract 21.72 g/L, culture temperature 30.84 °C and culture time 31.84 h. Under these conditions, the yield of AMEP412 was enhanced from 0.32 to 2.39 mg/mL (7.47-fold), which was quite close to the predicted one. This result significantly raised the yield of AMEP412, which laid solid foundation for its application as a biocontrol agent.

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Section: Discussionmentioning

confidence: 99%

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Liu

Shen

Yin

2021

Biotechnology & Biotechnological Equipment

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“…The fungal cellulase was produced from Trichoderma reesei. The optimum temperature found for bioconversion was 60 o C. Acharya (2010) studied saccharification of wheat bran by cellulases and concluded that endoglucanase and β-glucosidase retained 55 and 65 % activity at 60 o C. Increase in the temperature more than 60 o C resulted in a sharp decrease in the activity which can be attributed to thermal inactivation of the enzymes. Getting maximum activity at 60 o C at saccharification provided information that enzymes were performing their maximum during adsorption, desorption and hydrolysis at 60 o C. The stability of cellulases reported as the activity of cellulases which starts falling after 55 o C [Otter et al, 1984, Andreaus et al, 1999and Tu et al, 2009.…”

Section: Effect Of Temperature On Desorptionmentioning

confidence: 99%

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Baig¹

2021

Preprint

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Desorption of active cellulases from lignocellulosic substrates is a potential technique to reuse cellulases for the production of bioethanol. For desorption studies, adsorption of cellulases had to be performed first. Adsorption of cellulases NS 50013 onto microcrystalline cellulose (Avicel PH 101) and wheat straw lignin (Protobind 1000) was studied. It was found that Protobind adsorbed twice the amount of cellulases than Avicel did. An adsorption strategy developed was to work at pH 5 and a temperature less than 323 K to get maximum adsorption on the cellulose component, less adsorption on the lignin component of lignocellulosic materials, and to harmonize adsorption temperature with the industrial hydrolysis situation. Desorption of cellulases from Avicel and Protobind over a range of 298 K to 343 K and a pH of 6 to 9 was studied. Desorption obtained at pH 9 and 333K was optimum for both Avicel and Protobind. Hence, desorption was enhanced by 21 % and 11% for Avicel and Protobind respectively. The cellulases activity for Avicel was 48 FPU mL-1 at pH 9, 333 K, 5% glycerol, representing 91 % of the initial activity and for Protobind, the activity was 33 FPU mL-1 which represents about 66 % of the initial activity. All of these values were higher than ever reported in literature. At pH 5 and 298 K the amount of cellulases desorbed from untreated wheat straw (WS) was 33 % of those initially used for the adsorption step. It was increased to 42 % when 30 % delignified WS was used, and was further increased to 48 % for 60 % delignified WS. Desorption obtained for 60 % delignified WS was 75 % at pH 9, 333K and 5% glycerol. The desorption strategy recommended for bioethanol producing industries, is: 1) removal of lignin; 2) adsorption of cellulases at pH 5 and lower than 323K; 3) hydrolysis of lignocellulosic material; and 4) desorption of cellulases from non-hydrolyzed material at 333 K, pH 9, with 5-10 % glycerol. The proposed strategic desorption of cellulases may reduce the cost of Canadian bioethanol production by 26.5 % due to 75 % recyclability of active cellulases.

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“…Cellulase is industrially produced mainly by submerged fermentation (SmF) (Lin et al, 2017), although in the last two decades solid-state fermentation (SSF) has awakening interest due to the high enzyme productivity, reduced energy requirements, etc. (Acharya et al, 2010;Singhania et al, 2010). The scientific literature has reported studies concerning both solid-state and submerged fermentation (Hernández-Domínguez et al, 2014;Zanirun et al, 2014;Jasani et al, 2016;Bentil et al, 2018;Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

Pinotti¹,

Paulino²,

Agnezi³

et al. 2020

Afr. J. Biotechnol.

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Cellulases (enzymes of great potential in biotechnology) are currently of interest due to their applicability in the hydrolysis of cellulose from lignocellulosic materials, producing fermentable sugars for alcohol production. The great demand for efficient enzymes is the driving force to prospect new cellulases-producing microorganisms, as well as to optimize the enzyme production step. Many variables can be optimized in microorganism cultivation, such as pH, type of microorganism, induction, temperature, type and concentration of substrate, among others. This work aimed to evaluate the production of cellulases by submerged fermentation from three strains of filamentous fungi (Trichoderma koningii, Penicillium species, Rhizomucor species) and two strains of bacteria (Bacillus megaterium and Bacillus subtilis), using sugarcane bagasse as substrate. Variations in substrate concentrations (0.5, 1.6 and 2.7%, w/v) and temperature (28, 33 and 38°C) were evaluated on volumetric activity. The best fungus was T. koningii (3130.4 IU/L) using 2.7% natural sugarcane bagasse at 28°C. Among the bacteria, B. megaterium stood out with an enzyme production in range of 130 to 156.7 IU/L (at 28-33°C using natural and acid-alkaline pretreated bagasses), although up to around 20 times lower than the production by the T. Koningii.

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Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation

Cited by 41 publications

References 29 publications

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

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