Utilization of banana crop residue as an agricultural bioresource for the production of acetone‐butanol‐ethanol byClostridium beijerinckiiYVU1

Abstract

This study aimed to produce acetone‐butanol‐ethanol (ABE) using lignocellulosic crop residues as renewable bioresources. Butanol production from banana crop residue (BCR) was studied using a newly isolated solventogenic Clostridium beijerinckii YVU1. BCR is one of the abundant lignocellulosic substrates available in tropical countries containing 4·3 ± 3·5% cellulose, 28·5 ± 3·0% hemicellulose and 20·3 ± 2·6% lignin. The sequential dilute alkali and acid pretreatments solubilized 69% of lignin and 73% of hemicellulose. Ten percent (w/v) of pretreated substrate was subjected to enzymatic saccharification with cellulase, and it was found to release 0·481 ± 0·035 g glucose per g pretreated biomass. In the batch fermentation process, 20·5 g l−1 ABE (14·0 g l−1 of butanol, 5·4 g l−1 of acetone and 1·1 g l−1 of ethanol) was obtained. The executed fermentation process yielded 0·39 g ABE per g hydrolysate with 0·14 g l−1 h−1 of volumetric productivity. On the basis of the results, we believe that sequential alkali and acid pretreatment on the enzymatic hydrolysis for butanol production is indeed a technology with the potential to be applied and newly isolated. C. beijerinckii YVU1 is also a potential candidate organism for butanol production agricultural residues. Significance and Impact of the Study This study demonstrates that a banana crop residue (BCR) can be successfully utilized as an inexpensive and alternative bioresource for the production of acetone‐butanol‐ethanol (ABE). The sequential pretreatment of BCR with alkali and acid solubilized lignin and hemicellulose leading to high glucose release during enzymatic hydrolysis. A newly isolated Clostridium beijerinckii YVU1 was able to produce comparable amount of ABE with previous reports. Therefore, we can state that the utilization of BCR as substrate for C. beijerinckii YVU1 leads to an economical bioprocess for the microbial production of ABE.

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

“…To make a transition from these environmentally and human-threatening processes, scientists developed the acetone-butanol-ethanol fermentation route around the late 1800s and early 1900s [302,303], which traditionally used first-generation feedstocks like corn, cassava, sugarcane or wheat [304,305]. However, this process is still hindered by the low butanol yields and the high process costs [306,307]. Thus, recent studies are now using biomass residues (agricultural wastes, lignocellulosic wastes, and industrial wastes) alongside the cellulosic microbes found in various habitats to curb the process costs and pave the way for scale-up studies [308][309][310].…”

Comparative genome analysis of Clostridium beijerinckii strains isolated from pit mud of Chinese strong flavor baijiu ecosystem

“…Clostridium beijerinckii is a Gram-positive anaerobic solventogenic bacterium that is well known for its ability to produce acetone, butanol, and ethanol (ABE) or isopropanol, butanol, and ethanol (IBE) ( Qureshi and Blaschek 2001 ; Survase et al 2011 ; dos Santos Vieira et al 2020 ) using low-cost carbon sources, such as different straw hydrolysates ( Bellido et al 2014 ; Dalal et al 2019 ), lignocellulosic hydrolysate ( Reddy et al 2020 ), and molasses ( Li et al 2013 ; Fonseca et al 2020 ). In addition, it has been used to produce hydrogen with great success ( Seelert et al 2015 ).…”

Algae: The Reservoir of Bioethanol

“…According to the world energy outlook 2015, fossil fuels such as coal, petrol products and natural gas are being used predominantly for energy requirements in various sectors, providing more than 80% of the world's energy between 2013 and 2035 [2]. Specifically, energy demands across the industrial and transport sectors are high compared to other sectors [3,4]. The gap between demand and supply of fossil fuels is increasing continuously, thereby raising prices in recent days, which, in turn, affects the economy of the country [5,6].…”