Utilization of bentonite as a support material for immobilization of Candida rugosa lipase

Yeşiloğlu, Yeşim

doi:10.1016/j.procbio.2004.08.008

Cited by 74 publications

(39 citation statements)

References 26 publications

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“…Activated carbons are among the most effective adsorbents, having high surface area per unit mass (Yesiloglu 2005). Due to the relatively high cost of activated carbons, there have been attempts to utilize low cost, naturally occurring adsorbents for immobilization.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the relatively high cost of activated carbons, there have been attempts to utilize low cost, naturally occurring adsorbents for immobilization. In recent years, there has been increasing interest in utilizing natural clay minerals like montmorillonite (Ramos et al, 2014), bentonite (Yesiloglu 2005), and sepiolite (Myriam et al,1998) or natural polymers (Lv et al, 2008, Ittrat et al,2014, Pereira et al,2003, Ramani et al,2010 can also be used as support for the immobilization of enzyme. They are natural matrices resulting from agricultural waste, which contain cellulose, hemicellulose and lignin for this process.…”

Section: Resultsmentioning

confidence: 99%

“…Catalysis by Candida antarctica B (CALB) immobilized on natural pure silica by adsorption: comparison with the free enzyme contained in bentonite support (70 -80 %) (Yesiloglu 2005), and largely higher than is contained of montmorillonite (50-60 %) (Ramos et al, 2014), sepiolite (69.1%) and the palygorskite (70%) (Myriam et al, 1998). Biochemical properties of free and immobilized lipase pH stability and optimum pH: The effect of pH on the stability of both free and immobilized lipase was determined by measuring the residual conversion of oleic acid in the pH range of 6.0-8.0 and the results were presented in figure 1.…”

Section: Resultsmentioning

confidence: 99%

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Catalysis by <i>Candida antarctica</i> B (CALB) immobilized on natural pure silica by adsorption: comparison with the free enzyme

Djossou¹,

Mazou²,

Toukourou³

et al. 2017

J. App. Bioscience.

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Objective:In this work, a lipase B from Candida antarctica strain was immobilized onto natural silica carriers via adsorption to enhance its feasibility in practical applications. Methodology and results:The biocatalyst was prepared by simple adsorption on the support whose composition was beforehand characterized and the activities in ethyl ester hydrolysis and synthesis evaluated. The natural silica carriers were rich in silicon dioxide (SiO2) (97.36%). The optimum temperature of immobilized lipase was 40 °C, which was identical to that of the free enzyme. The immobilized lipase exhibited a higher relative activity than that of free lipase over 40 °C. The optimal pH for immobilized lipase was 7.5, which was similar to that of the free enzyme. After 10 recycled batches, a high residual esterification conversion (90%) was maintained. Conclusions and application of findings:The results in the present work indicate that pure silica is a potential material as an immobilization matrix for industrial process. There may have a promising future as support of the biocatalysts in various syntheses.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Catalysis by <i>Candida antarctica</i> B (CALB) immobilized on natural pure silica by adsorption: comparison with the free enzyme

Djossou¹,

Mazou²,

Toukourou³

et al. 2017

J. App. Bioscience.

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“…O grande problema deste método é a elevada toxicidade dos reagentes formadores de ligações cruzadas. 23,24 Ligação covalente: essa forma de imobilização consiste na ligação covalente dos grupos funcionais dos componentes biológicos e dos grupos reativos do seu suporte. Primeiramente, o suporte é ativado com um reagente específico, e, em seguida, o componente biológico é adicionado formando a ligação.…”

Section: Ligaçãounclassified

“…A principal desvantagem desse método é a perda de material biológico pelos poros dos polímeros. 24 Além das técnicas de imobilização, é importante ressaltar algumas características que são desejadas quando se fala de biossensores. Entre elas está uma alta sensibilidade, o que faz com que baixas concentrações, como na escala de partes por bilhão, possam ser detectadas.…”

Section: Ligaçãounclassified

Biosensor and Food Industry - Review

Oliveira

Pereira

2016

Rev. Virtual Quim.

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Biosensors are devices that combine a biological recognition element with a transducer. The biological element has the property of recognizing and selectively interacting with the analyte. It can be used on the surface of the sensor micro-organisms, antibodies, nucleic acids, cells, organelles, proteins, enzymes, among others. The interaction results in the modification of one or more physical-chemical properties that are detected and measured by the transducer. The main objective is to produce an electronic signal proportional to the concentration of the analyte or analytes group that interact with the biological component. Applications of biosensors can be found in different areas of knowledge: livestock, food, agronomic and others. In the case of food quality analysis, biosensors are applied, particularly in the detection of chemical and biological contaminants, such as pesticides, antibiotics, and various micro-organisms. The advantages of biosensors over conventional techniques is not limited sensitivity and selectivity, but in the fact of usually dispenses a pre-treatment of the sample (practical), speed in analysis and minimum reagent costs, thus providing flexibility in obtaining the results and reduce of financial cost. Thus, this review aims to evaluate the application of biosensors in food samples and the progress of analytical methodology today.Keywords: Biosensor; Chemical Compound; Biological Compound. ResumoOs biossensores são dispositivos que combinam um elemento biológico com um transdutor. O elemento biológico tem a propriedade de reconhecer e interagir seletivamente com o analito. Podem ser empregados na superfície do sensor micro-organismos, anticorpos, ácidos nucleicos, células, organelas, proteínas, enzimas, entre outros. A interação resulta na alteração de uma ou mais propriedades físico-químicas que são detectadas e medidas pelo transdutor. O principal objetivo é produzir um sinal eletrônico proporcional à concentração de um determinado analito ou grupo de analitos que interagem com o componente biológico. Aplicações dos biossensores podem ser encontradas em diferentes áreas do conhecimento: pecuária, alimentos, agronômica e outras. No caso da análise de qualidade de alimentos, os biossensores são aplicados, sobretudo, na detecção de contaminantes químicos e biológicos, tais como pesticidas, antibióticos, e micro-organismos diversos. As vantagens dos biossensores em relação às técnicas convencionais não se limitam à sensibilidade e seletividade, mas ao fato de, geralmente, dispensarem um elaborado pré-tratamento da amostra (praticidade), rapidez nas análises e gastos mínimos de reagentes, proporcionando assim, agilidade na obtenção dos resultados, e redução no custo financeiro. Assim, a presente revisão tem como objetivo avaliar a aplicação de biossensores em amostras de alimentos e o progresso dessa metodologia analítica nos dias atuais.

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Use of ionic liquids as additives for the immobilization of lipase from Bacillus sp.

Padilla

Melo

Pereira

et al. 2014

J of Chemical Tech & Biotech

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BACKGROUND Ionic liquids (ILs) have been used as additives to immobilise lipase from a new source of Bacillus sp. (ITP‐001) by the physical adsorption method supporting green poly(3‐hydroxybutyrate‐co‐hydroxyvalerate) (PHBV) in order to evaluate the influence of the cationic core ([C4mpy]Cl, [C4min]Cl), of anions ([C4min]Cl, [C4min]N(CN)2, [C4min]Tf2N) and the cation chain length ([C2min]Tf2N, [C4min]Tf2N)) in the immobilization process. The immobilized biocatalysts (IB) were characterized with regard to morphological and physico‐chemical properties, and total activity recovery yield (Ya) and biochemical properties of more efficient IB were evaluated. RESULT Total activity recovery yield (Ya) for the immobilized biocatalysts employing LI as additives always resulted in higher values compared with the control. The most efficient was using the more hydrophobic IL [C4min]Tf2N, which represents a 2‐fold greater increase in the recovery of enzymatic activity. It retained more than 73% of its original activity after 20 reuses compared with the control, which, after ten cycles, retained only 33% of its original activity. CONCLUSION The anions and the cation chain length of ionic liquids have a strong influence on the immobilization process. The results for biocatalyst immobilized with [C4min]Tf2N showed an increase in total activity recovery yield and considerable improvement in operational stability. © 2014 Society of Chemical Industry

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Utilization of bentonite as a support material for immobilization of Candida rugosa lipase

Cited by 74 publications

References 26 publications

Catalysis by <i>Candida antarctica</i> B (CALB) immobilized on natural pure silica by adsorption: comparison with the free enzyme

Catalysis by <i>Candida antarctica</i> B (CALB) immobilized on natural pure silica by adsorption: comparison with the free enzyme

Biosensor and Food Industry - Review

Use of ionic liquids as additives for the immobilization of lipase from Bacillus sp.

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