Utilization of Coconut Milk Skim Effluent (CMSE) as Medium Growth for Spirulinaplatensis

Abstract

Virgin coconut oil (VCO) is commonly known as one of coconut products in Indonesia. Along with the production, abyproduct coconut milk skim effluent (CMSE) is also generated and could become a potential pollutant for the environment. However the effluent still contain micro and macronutrient that could be utilized for microalgae growth. The purpose of this research was to determine wastewater characterization and to utilize the wastewater as Spirulinaplatensis medium. Cultivation was maintained in pH=9, 2 ppt salinity, for 5 days under flourescent lamp 5000 lux. CMSE was added in different concentration (10-80%v/v) and different synthetic medium reduction (0-50%w/v). The result indicated that 20% v/v medium and 10%v/w synthetic medium provided optimum growth, produced up to 0.206 gr/l dry weight of biomass and contained 39% of protein.

Characteristics of coconut protein separation process by means of membrane ultrafiltration

“…The electrokinetic potential at the interface between the diffuse layer of a certain particle and its dispersion medium or the zeta (ζ)‐potential was characterized by laser Doppler electrophoresis. The sample was diluted to 0.01% (w/w) total solid and adjusted in the certain range of pH (Anagnostou, ; Azimatun Nur et al, ; Bawalan, ; Bawalan & Chapman, ; Belfort, Davis, & Zydney, ; Breite, Went, Prager, & Schulze, ; Choi, Zhang, Dionysiou, Oerther, & Sorial, ; Das, ; Garcia, Arocena, Laurena, & Tecson‐Mendoza, ; Giri & Mangaraj, ) by using 0.1 M HCl and 0.1 M NaOH solution. ζ‐Potential disposable cell containing an aliquot of the sample was placed in Nano patica SZ‐100, Horiba.…”

“…However, parallel to the production of VCO wet process, coconut skimmed effluent (CSE) is usually generated in relatively large quantity. This by‐product was claimed as a comparably low to no market value which is usually conveyed to the wastewater treatment then discharged (Bawalan & Chapman, ), but owing to the nutritional compositions in CSE especially the proteins that responsible for more than 20% dry basis of the constituents mixed in the CSE (Ng, Mohammad, Ng, & Jahim, ; Wetwitayaklung, ), this effluent has been kindly noted to be utilized potentially as an alternative source of protein or as a food additive (Azimatun Nur, Irawan, & Hadiyanto, ; Onsaard, Vittayanont, Srigam, & McClements, ; Tripetchkul, Kusuwanwichid, Koonsrisuk, & Akeprathumchai, ). Coconut protein was reported to be capable to significantly upregulate the cardio‐protective and anti‐diabetic activities in rats due to the richness of antioxidant arginine.…”