Microalgae cultivated in mixotrophic conditions have received significant attention as a suitable source of biofuel feedstock, based on their high biomass and lipid productivity. POME is one of the wastewaters generated from palm oil mills, containing important nutrients that could be suitable for mixotrophic microalgae growth. The aim of this research was to identify the growth of Chlorella vulgaris cultured in POME medium under mixotrophic conditions in relation to a variety of organic carbon sources added to the POME mixture. The research was conducted with 3 different carbon sources (D-glucose, crude glycerol and NaHCO 3) in 40% POME, monitored over 6 days, under an illumination of 3000 lux, and with pH = 7. The biomass was harvested using an autoflocculation method and dry biomass was extracted using an ultrasound method in order to obtain the lipid content. The results show that C. vulgaris using D-glucose as carbon source gained a lipid productivity of 195 mg/l/d.

A rapid method using isoelectric focusing (IEF) was developed to identify species of cooked snapper. The IEF protein band patterns of red snapper as impacted by two extraction systems (water and 8M urea), various cooking times (raw, 5, 10, 15, 20, 25 and 30 min) and cooking methods (microwave, bake, steam, broil, deep fry, and pan fry) were investigated. Samples were desalted and cleaned using a 10-sec rinsing procedure. The characteristic IEF protein pattern of red snapper was reproducible for various cooking conditions. Both 8M urea and water could be used as extractant of snapper sarcoplasmic proteins. IEF protein bands in the acidic region of the polyacrylamide gel (pH 3.5-9.5) were useful as markers for species identification.

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Waste of Virgin Coconut Oil (VCO) industries has a high value of Chemical Oxygen Demand (COD), it is therefore a problem for the environment. This waste contains organic materials such as oil, proteins, carbohydrates and some minerals, that potentially to be used for the cultivation of blue-green algae Spirulina plantesis.  Spirulina plantesis is an excellent source of phycocyanin. The Phycocyanin has anti-aging, anti-oxidant and anti-inflammatory properties, it is therefore considered useful. This research is aimed to study the effects of the addition of urea and light intensity on the growth of Spirulina, the concentration of phycocyanin and decreasing of COD value. Spirulina platensis was cultivated in a closed photobioreactor with an air flow for 7 days.  The light intensity was varied as follows 5000 lux, 6000 lux, 7000 lux and 8000 lux, and the addition of urea as nutrients was also varied as follows 40 ppm, 50 ppm, 60 ppm and 70 ppm.  This research indicated that the optimum condition was obtained at the addition of urea of 70 ppm, light intensity  of 6000 lux. This research resulted μmax of 1,1375day-1 , the biomass  productivity of 0,0423 g/l/day,  biomass concentration of   0,1734 g/l  and  phycocyanin concentration of 5,047%. The largest of COD removal is was 98,06% and the COD value of  280 mg/l was finally achieved.

Virgin coconut oil (VCO) is commonly known as one of coconut products in Indonesia. Along with the production, abyproduct coconut milk skim effluent (CMSE) is also generated and could become a potential pollutant for the environment. However the effluent still contain micro and macronutrient that could be utilized for microalgae growth. The purpose of this research was to determine wastewater characterization and to utilize the wastewater as Spirulinaplatensis medium. Cultivation was maintained in pH=9, 2 ppt salinity, for 5 days under flourescent lamp 5000 lux. CMSE was added in different concentration (10-80%v/v) and different synthetic medium reduction (0-50%w/v). The result indicated that 20% v/v medium and 10%v/w synthetic medium provided optimum growth, produced up to 0.206 gr/l dry weight of biomass and contained 39% of protein.