Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate <i>Isochrysis galbana</i> to carbon or nitrogen stress

Clark, Darren R.; Merrett, M. J.; Flynn, Kevin J.

doi:10.1046/j.1469-8137.1999.00539.x

Cited by 16 publications

(21 citation statements)

References 31 publications

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“…A rise in C:N ratios with increasing CO 2 , such as that found here in 2 centric diatoms and Isochrysis glabana, was observed previously in marine and freshwater phytoplankton (Clark et al 1999, Tortell et al 2000, Clark 2001, Urabe & Waki 2009, Montechiaro & Giordano 2010. The lack of an effect of CO 2 on C:N in Phaeodactylum tricornutum is consistent with previous studies of pennate diatoms which showed little to no effect of CO 2 on C:N ratios (Burkhardt et al 1999, Sun et al 2011.…”

Section: Co 2 Regulation Of Element Ratios In Marine Phytoplanktonsupporting

confidence: 92%

Carbon dioxide regulation of nitrogen and phosphorus in four species of marine phytoplankton

Reinfelder¹

2012

Mar. Ecol. Prog. Ser.

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The concentration of carbon dioxide in seawater may affect phytoplankton physiology and ecology and their role in marine biogeochemical cycles. In order to assess the effects of CO 2 on the elemental composition of marine phytoplankon, carbon, nitrogen, and phosphorus quotas were measured in 4 species of marine phytoplankton acclimated to 150 to 1500 ppm CO 2 (5 to 50 µM) in semi-continuous cultures. Nitrogen quotas declined steeply with increasing CO 2 in the centric diatoms Thalassiosira pseudonana and T. weissflogii acclimated to 150 to 380 ppm (5 to 13 µM), but more slowly as the CO 2 increased from 380 to 1500 ppm (13 to 50 µM). Nitrogen demand varied little with CO 2 in the pennate diatom Phaeodactylum tricornutum, but was positively correlated with CO 2 over the range of 150 to 770 ppm in the prymnesiophyte Isochrysis galbana. Based on the nitrogen−CO 2 trends in centric diatoms, relief from carbon−nitrogen colimitation could lead to 2-fold larger cells as CO 2 increases from 150 to 380 ppm, but only 15% larger cells from 380 to 770 ppm CO 2 . Phosphorus quotas in the 3 diatoms decreased as CO 2 increased from 150 to 380 ppm. As previously observed in these and other species, C:N, C:P, and N:P ratios increased with increasing CO 2 , but the present results show that much of this variation was due to differences in nitrogen and phosphorus rather than carbon quotas. Marine phytoplankton could provide a negative feedback against increasing CO 2 over the pCO 2 range of 150 to 380 ppm by supporting larger cells or higher biomass, but would support a smaller carbon sink as atmospheric CO 2 rises above 380 ppm. KEY WORDS: Nitrogen · Phosphorus · Carbon dioxide · PhytoplanktonResale or republication not permitted without written consent of the publisher

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Section: Co 2 Regulation Of Element Ratios In Marine Phytoplanktonsupporting

confidence: 92%

Carbon dioxide regulation of nitrogen and phosphorus in four species of marine phytoplankton

Reinfelder¹

2012

Mar. Ecol. Prog. Ser.

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“…1A and B) and was accompanied by relevant changes in the biochemical composition of cells. A comparable increase in the C:N ratio has been observed in the haptophyte Isochrysis galbana Parke as a response to N stress (Clark et al 1999). Nonetheless, no significant variations of the pH/CO 2 profiles during darkness, which can be considered as an indirect estimation of dark respiration rates, were detected (not shown) for the studied treatments.…”

Section: Discussionsupporting

confidence: 66%

Growth and Biochemical Composition of the Diatom Phaeodactylum tricornutum at Different pH and Inorganic Carbon Levels under Saturating and Subsaturating Light Regimes

Bartual¹,

Gálvez²

2002

Botanica Marina

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“…P. tricornutum cultures have also been proven to approximately double biomass production under 2 % CO 2 incubation (Peng et al 2014). On the other hand, I. galbana cultures have also been shown to increase culture cell density with increasing concentrations of inorganic carbon (Clark et al 1999). This information suggests that the cell densities observed in the present study could be further incremented with the addition of carbon sources.…”

Section: Agitationsupporting

confidence: 61%

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

2015

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Fucoxanthin is a carotenoid that exerts multiple beneficial effects on human health. However, reports comparing microalgae culture conditions and their effect on growth and fucoxanthin production are still limited. Isochrysis galbana and Phaeodactylum tricornutum cultures in different light (62.0, 25.9, 13.5, or 9.1 μmol photons m -2 s -1 ), mixing conditions (1 vvm aeration or 130 rpm agitation), and media compositions (F/2 and Conway medium) were studied for comparison of cellular growth and fucoxanthin production on F/2 medium. I. galbana showed a better adaptation to tested culture conditions in comparison with P. tricornutum, reaching 2.15 × 10 7 ±4.07×10 6 cells mL -1 and a specific growth rate (μ) of 1.12±0.05 day -1 under aerated conditions and 62.0 μmol photons m -2 s -1 light intensity. Fucoxanthin concentration was about 25 % higher in P. tricornutum cultures under 13.5 μmol photons m -2 s -1 light intensity and aerated conditions, but the highest fucoxanthin total production was higher in I. galbana, where 3.32 mg can be obtained from 1 L batch cultures at the 16th day under these conditions. Moreover, higher cell densities (~32.41 %), fucoxanthin concentration (~42.46 %), and total production (~50.68 %) were observed in I. galbana cultures grown in Conway medium, if compared with cultures grown in F/2 medium. The results show that the best growth conditions did not result in the best fucoxanthin production for either microalgae, implying that there is not a direct relationship between cellular growth and fucoxanthin production. Moreover, the results suggest that I. galbana cultures on Conway medium are strong candidates for fucoxanthin production, where 1.2 to 15 times higher fucoxanthin concentration are observed in comparison to macroalgal sources.

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Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress

Cited by 16 publications

References 31 publications

Carbon dioxide regulation of nitrogen and phosphorus in four species of marine phytoplankton

Carbon dioxide regulation of nitrogen and phosphorus in four species of marine phytoplankton

Growth and Biochemical Composition of the Diatom Phaeodactylum tricornutum at Different pH and Inorganic Carbon Levels under Saturating and Subsaturating Light Regimes

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

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