Utilization of Microfluidic Device for Determination of Nitrite and Nitrate in Water and Soil Samples

Abstract

Nitrate and nitrite species are naturally occur in typically environment and are part of the nitrogen cycle. They represent wide spread contaminants in the environment and important serve to evaluate quality of natural water 1 . Naturally occurring nitrate levels in surface and ground waters are generally a few mg/L. In many ground waters, an increase of nitrate levels has been observed due to the excess application of nitrogen fertilizers of farming practice 2 . In some countries, up to 10 % of the pollution may be exposed to nitrate levels in drinking water of above 50 mg/L. As recently found in 2007, the Greenpeace Research Group has reported the nitrate levels found in agricultural areas in Thailand. Drinking water from 30 % of all groundwater wells in investigated areas showed high content of nitrates which are 3 times above the World Heath Organization (WHO) safety limit of 50 mg/L of nitrate. This nitrate pollution was highest in the most intensive crops, in asparagus farms in Kanchanaburi 3 . High levels of nitrate

Quantitative Monitoring of Rutin in Human Urine by Flow Injection-Chemiluminescence Analysis

“…Since Albrecht discovered luminol in 1928, 1 this compound has been one of the most frequently used chemiluminescence (CL) reagents. Luminol-based flow injection-CL (FI-CL) system has been continuously making great progress and broadening its practical applications [2][3][4][5] in different fields, e.g., environmental monitoring, 6,7 pharmaceuticals testing, [8][9][10] food safety detection, 11,12 clinical immunoassays, 13 and trace metal assays. 14 Protein-small molecule interaction has become a hot spot in the fields of biology, medicine, environmental science, and chemistry in recent decades.…”