Utilization of Mn‐Doped ZnSe/ZnS Core/Shell Quantum Dots for Rapid Detection of Escherichia coli O157:H7 and Methicillin‐Resistant Staphylococcus aureus

Abstract

In this study, Mn-doped ZnSe/ZnS core/shell quantum dots (CSQDs) were synthesized in aqueous solution using polyethylene glycol as a surface stabilizer and successfully applied in the detection of Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) for the first time. The CSQDs were conjugated with anti-E. coli antibody and anti-MRSA antibody via protein A supported by 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride for fluorescent labeling of the intact bacterial cells. The detection was performed for the bacterial strains cultivated in Luria-Bertani liquid medium. The obtained results indicate that E. coli O157:H7 and MRSA can be detected within 30 min at a high sensitivity of 101 CFU/mL. This labeling method based on the highly fluorescent CSQDs may have great potential for use in the food industry to check and prevent outbreaks of foodborne illness.

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

“…40−42 Bui et al prepared more stable Mn-Doped ZnSe/ZnS QDs in solution by improving the previous complex synthesis method of core/shell QDs, and combined with antibodies to detect MRSA. 43 The QDs coupled with anti-MRSA antibody could complete the labeling of MRSA within 30 min. The sensitivity of this rapid and simple detection method is 10 CFU/mL.…”

“…Quantum dots (QDs), a nanoparticle with optical properties, have aroused strong interest of researchers due to excellent fluorescent property, bioimaging, and so on. , As nanomaterial and fluorescence signal, QDs has achieved excellent results in pathogens detection, bacteriostasis, and sterilization in recent years. − Bui et al prepared more stable Mn-Doped ZnSe/ZnS QDs in solution by improving the previous complex synthesis method of core/shell QDs, and combined with antibodies to detect MRSA . The QDs coupled with anti-MRSA antibody could complete the labeling of MRSA within 30 min.…”