Utilization of Nutrients by Isolated Epithelial Cells of the Rat Colon

Roediger, W. E. W.

doi:10.1016/s0016-5085(82)80339-9

Cited by 1,051 publications

(434 citation statements)

References 20 publications

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“…However, for the porcine species, precise immunolocalisation along the intestinal tract has not been performed thus far. MCT1 is known to also transport other SCFA (Halestrap and Meredith, 2004) but in the large intestine, butyrate is the preferred fuel for colonic epithelial cells (Roediger, 1982). Its transport depends on MCT1 which is supposed to be necessary to maintain metabolic energy at both cytoplasmic and nuclear level (Cuff et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Welter

Claus

2008

Cell Biology International

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Uptake of energy into cells and its allocation to individual cellular compartments by transporters are essential for tissue homeostasis. The present study gives an analysis of MCT1 expression and its cellular occurrence in the porcine intestine. Tissue portions from duodenum, jejunum, ileum, colon ascendens, colon transversum and colon descendens were collected and prepared for immunohistochemistry, Western blot and real time RT-PCR. A 169bp porcine MCT1 cDNA fragment was amplified and published. MCT1 mRNA expression in the large intestine was 20 fold higher compared to the small intestine. Western blot detected a single protein band of 41kDa at a much higher amount of MCT1 protein in the large intestine vs. the small intestine. MCT1 protein was detected in mitochondrial fractions of the large but not the small intestine. Immunohistochemistry in the small intestine showed that immune cells in the lamina propria and in the lymphoid follicles primarily expressed MCT1 while in the colon epithelial cells were the main source of MCT1. In summary, cellular expression of MCT1 differs between epithelial cells in the colon and small intestine. A possible role of MCT1 for uptake of butyrate into immune cells and the overall role of MCT1 for intestinal immune cell function remains elusive.

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Section: Discussionmentioning

confidence: 99%

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Welter

Claus

2008

Cell Biology International

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“…Butyrate serves as the major respiratory fuel for colonic epithelial cells (Roediger, 1982), enhances mucosal barrier integrity by downregulating expression of pro-inflammatory genes (McDermott & Huffnagle, 2014) and is essential to the maintenance of homeostasis in the colonic epithelium (Bugaut & Bentéjac, 1993;Treem et al 1994;Hamer et al 2008). As such, butyrate is of fundamental importance to the health of colonic mucosa, and alteration in its luminal production or supply is associated with a variety of colonic disorders (Soergel, 1994;Thibault et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Characterization of butyrate transport across the luminal membranes of equine large intestine

Nedjadi

Moran

Al-Rammahi

et al. 2014

Experimental Physiology

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New Findings r What is the central question of this study?Butyrate, a product of colonic microbial fermentation of dietary fibre (grass), is a major source of energy for the horse and plays an important role in maintaining the health of the intestine. What are the properties of the membrane protein and what is the mechanism by which butyrate is absorbed in equine large intestine (colon)? r What is the main finding and its importance?We have identified the mechanism of and membrane protein involved in butyrate transport in equine large intestine. This knowledge will allow rational approaches to the design of dietary formulations to enhance butyrate production and absorption in equine colon, in order to provide more energy for the horse and maintain its gut health.The diet of the horse, pasture forage (grass), is fermented by the equine colonic microbiota to short-chain fatty acids, notably acetate, propionate and butyrate. Short-chain fatty acids provide a major source of energy for the horse and contribute to many vital physiological processes. We aimed to determine both the mechanism of butyrate uptake across the luminal membrane of equine colon and the nature of the protein involved. To this end, we isolated equine colonic luminal membrane vesicles. The abundance and activity of cysteine-sensitive alkaline phosphatase and villin, intestinal luminal membrane markers, were significantly enriched in membrane vesicles compared with the original homogenates. In contrast, the abundance of GLUT2 protein and the activity of Na + -K + -ATPase, known markers of the intestinal basolateral membrane, were hardly detectable. We demonstrated, by immunohistochemistry, that monocarboxylate transporter 1 (MCT1) protein is expressed on the luminal membrane of equine colonocytes. We showed that butyrate transport into luminal membrane vesicles is energized by a pH gradient (out < in) and is not Na + dependent. Moreover, butyrate uptake is time and concentration dependent, with a Michaelis-Menten constant of 5.6 ± 0.45 mm and maximal velocity of 614 ± 55 pmol s −1 (mg protein) −1 . Butyrate transport is significantly inhibited by p-chloromercuribenzoate, phloretin and α-cyano-4-hydroxycinnamic acid, all potent inhibitors of MCT1. Moreover, acetate and propionate, as well as the monocarboxylates pyruvate and lactate, also inhibit butyrate uptake. Data presented here support the conclusion C

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“…Dietary wheat bran results in higher conccntrations of butyrate than either oat bran or guar gum (McIntyre er al., 1993). Butyrate is preferentially taken up by the colonic epithelium, where it is actively mctabolised to producc energy (Roediger, 1982). Butyrate is, therefore, an important energy source for thc colonocyte and is metaboliscd in prefcrence to glucose o r glutamine, accounting for 70?6 of the total energy consump-tion (Scheppach, 1994).…”

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confidence: 99%

Apoptosis in colorectal tumour cells: Induction by the short chain fatty acids butyrate, propionate and acetate and by the bile salt deoxycholate

Hague¹,

Elder²,

Hicks³

et al. 1995

Intl Journal of Cancer

388

253

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The short chain fatty acids acetate, propionate and butyrate are produced when dietary fibre is fermented by the colonic bacteria. We have previously shown that sodium butyrate induces apoptosis in 3 colorectal turnour cell lines. We have extended our study to 3 adenoma and 4 carcinoma cell lines and investigated whether propionate and acetate also induce apoptosis. All 3 short chain fatty acids induced apoptosis at physiological concentrations, but of the 3. butyrate was the most effective.

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Utilization of Nutrients by Isolated Epithelial Cells of the Rat Colon

Cited by 1,051 publications

References 20 publications

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Characterization of butyrate transport across the luminal membranes of equine large intestine

Apoptosis in colorectal tumour cells: Induction by the short chain fatty acids butyrate, propionate and acetate and by the bile salt deoxycholate

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