Utilization of pineapple peel and rice washing water to produce single cell proteins using Saccharomyces cerevisiae

Abstract

Single cell protein (SCP) is an alternative for meeting protein needs in the future. The wastes such as pineapple skin and rice water washing can be utilized to produce SCP. These two materials are abundant in Indonesia. The consumption of pineapple and rice in Indonesia is very high. The rice is the main food for Indonesian people. The aim of this study is to determine the best ratio of pineapple skin juice and rice washing water as a growing medium to produce single cell proteins. This study was performed in a completely randomized design with two factors. The first factor was the ratio of pineapple skin and rice washing water. The ratios between pineapple skin and rice washing water were 1: 1, 2: 1 and 1: 2. The second factor was fermentation times (8, 24, 32, 48, 56, 72 and 80 hours). The analysis carried out included pH, cell dry weight and protein content. The best treatment is obtained by the treatment of pineapple skin and rice washing water in ratio of 1: 2 when it is fermented for 56 hours. This media produced 0.4752 grams of cell dry weight with the protein content at about 289.08 ppm.

Opportunities and challenges in single‐cell protein production using lignocellulosic material

“…With the addition of fructose, it had a pH of 3.81, a protein content of 69.08 mg L −1 and a dry weight of 0.4203 g, whereas with the addition of sucrose, the pH was 4.33, the protein content of 85.55 mg L −1 , and the dry weight of the cells was 0.3385 g. Therefore, adding fructose as a carbon source results in a greater cell dry weight than adding sucrose carbon. Mujdalipah et al 65 reported using pineapple peels and rice water washings to produce SCP by employing S. cerevisiae. The main objective of this work was to find the effect of the ratio of pineapple and rice waste.…”

Single Cell Protein Production Using Different Fruit Waste: A Review

“…Watermelon, mixture of fruit wastes SF/LSF [89] Watermelon, pineapple SF/LSF [90] Yam peel SF/LSF [91] Apple, orange peel SF/LSF [36] Cucumber peel, orange peel SF/LSF [20] Pineapple waste SF/LSF [11,46,92,93] Papaya waste SF/LSF [94] Apple, papaya, banana SF/LSF [77] Guava peels and cashew bagasse SSF [95] Rind of pomegranate, mango, banana, apple, sweet orange peel SSF [79] Orange peels SSF [96,97] Pichia pinus Mango waste SF/LSF [98]…”

Review on Production of Single-Cell Protein from Food Wastes

“…Liquid substrates reported are cheese whey (Yadav et al, 2015;Monkoondee et al, 2016), non-dairy creamer wastewater (Dewi et al, 2017), sugar refinery wastewater (Saejung and Salasook, 2020), pineapple peel juice, and rice washing water (Mujdalipah and Putri, 2020), latex rubber sheet wastewater (Kornochalert et al, 2014), dephenolized olive mill wastewater (Giavasis and Petrotos, 2016), sugarcane molasses (Hansen and Cheong, 2019;Spalvins et al, 2018a), soybean molasses (Gao et al, 2012), deproteinized leaf juice (Chanda and Chakrabarti, 1996;Spalvins et al, 2018a), food waste-derived volatile fatty acids (Wainaina et al, 2020), waste milk (Myint et al, 2020), municipal wastewater treatment effluent (Steinberg et al, 2017), food processing wastewater (Xu et al, 2020). Urban bio-waste is also a potential substrate for SCP production after anaerobic digestion; methane produce during digestion can serve as a carbon source in the production of SCP rich in essential amino acids (Khoshnevisan et al, 2019).…”

“…Similar pre-treatment was also reported by Saejung and Salasook (2020) in the production of biomass using sugarcane processing wastewater. Filtration and sterilization were reported by Mujdalipah and Putri (2020) in pineapple peel juice and rice washing water. Giavasis and Petrotos (2016) reported dephenolized by microfiltration and condensation by reverse osmosis as pre-treatment operations for olive mill wastewater.…”