Utilization of Solid Waste as a Substrate for Production of Oil from Oleaginous Microorganisms

Abstract

The overwhelming demand of oil and fats to meet the ever increasing needs for biofuel, cosmetics production, and other industrial purposes has enhanced a number of innovations in this industry. One such innovation is the use of microorganisms as alternative sources of oil and fats. Organic solid waste that is causing a big challenge of disposal worldwide is biodegradable and can be utilized as substrate for alternative oil production. The study evaluated the potential of isolated yeast-like colonies to grow and accumulate oil by using organic solid waste as substrate. Of the 25 yeast-like colonies isolated from the soil samples collected from three different suburbs in Kampala district, Uganda, 20 were screened positive for accumulation of lipid but only 2 were oleaginous. The NHC isolate with the best oil accumulation potential of 48.8% was used in the central composite design (CCD) experiments. The CCD experimental results revealed a maximum oil yield of 61.5% from 1.25 g/L cell biomass at 10 g/L of solid waste and temperature of 25°C. The study revealed that organic solid waste could be used as a substrate for microbial oil production.

Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

“…Based on the fatty acid profile, microbial oils could have various applications to produce biodiesel, surfactants, waxes, lubricants and chemical feedstocks [58,59]. Oleaginous yeast has been proven to contribute to the sustainable biodiesel industry as studies showed that it can successfully accumulate microbial oil with similar composition to the conventional plant oil, which is composed mainly of oleic acid (C18:1), stearic acid (C18:0) and palmitic acid (C16:0), and low amounts of linoleic, linolenic and palmitoleic acid on substrates such as industrial glycerol, solid waste, sewage sludge or sugar cane molasses [30,[60][61][62].…”

Biofuel Application as a Factor of Sustainable Development Ensuring: The Case of Russia

“…At the same time, the applied technological solutions in the production of BF allowed obtaining an additional some valuable by-products (for instance, glycerin in biodiesel production) which are used in the pharmaceutical, cosmetic and chemical industry. As a result, the by-products began to contribute to the added value generated by the production of certain types of BF [36,56].…”