Utilization of tannery solid waste for protease production by Synergistes sp. in solid‐state fermentation and partial protease characterization

Abstract

Synergistes sp. DQ560074 produced a protease in submerged fermentation (SmF) at 400–420 U/mL and in solid‐state fermentation (SSF) at 745–755 U/g. The protease, which belongs to the aspartic protease class, was active over a wide range of pH (5–7) and at high temperatures (25–45°C). The protease is stable and active in various polar protic solvents (50% v/v) like ethanol, isopropanol, n–butanol, in polar aprotic solvents (50% v/v) like acetonitrile, and in non‐polar solvents (50% v/v) such as ethylacetate and toluene, but not in hydrophilic organic solvents (methyl alcohol and acetone). As far as we know, this is the first contribution to the production of a mesophilic protease with solvent stability in SSF using a proteinaceous solid waste.

From Wastes to High Value Added Products: Novel Aspects of SSF in the Production of Enzymes

“…Recently, the production of enzymes on solid state fermentation has been implemented in order to reduce the costs involved, especially if residues are used as substrates, and enhance the field of application (Sandhya et al, 2005;Kumar et al, 2009). However, there is indeed few research work developed on bench scale solid substrate fermentation, as the majority of this research was conducted on few grams of substrate materials,…”

“…Therefore, these waste materials are creating a solid waste disposal problem in tanneries. Kumar et al (2009) studied the use of animal fleshings, the proteinaceous part of tannery solid wastes, as substrate for the production of aspartic protease by a Synergistes sp. It was suggested that there is a possibility to produce this enzyme by SSF using a cheap substrate and moreover, the enzyme obtained exhibited high stability in various organic solvents.…”

Industrial wastes and sludges management by vermicomposting

“…Tanning industries generate approximately 1,50,000 tonnes of tanning wastes in the form of raw hide (and skin) trimmings, limed animal fleshings, hide splits and chrome shavings per annum during leather processing (Ganesh Kumar et al 2009). Tannery sludge contains plant macro and micronutrients especially nitrogen, phosphorus, iron, zinc and copper.…”

Bacterial composting of animal fleshing generated from tannery industries

“…Tanning industry is one of the highly polluting and growth-oriented industries and it generates approximately 1,50,000 tonnes of offal's in the form of raw hide (and skin) trimmings, limed animal fleshings, hide splits and chrome shavings per annum during leather processing (Ganesh Kumar et al, 2009). Animal fleshing (ANFL) is one of the major solid wastes that are generated during pretanning operations of leather processing.…”