Utilizing DNA metabarcoding to characterize the diet of marine-phase Arctic lamprey (<i>Lethenteron camtschaticum</i>) in the eastern Bering Sea

Shink, Katie G.; Sutton, Trent M.; Murphy, James M.; López, J. Andrés

doi:10.1139/cjfas-2018-0299

Cited by 18 publications

(15 citation statements)

References 47 publications

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“…Recently, DNA metabarcoding of gut contents was used qualitatively to characterize species composition of the diet of Arctic Lamprey Lethenteron camtschaticum in 556 the ocean (Shink et al 2019), and this same technology could potentially be useful in detecting consumed larvae in the guts of lamprey predators. Use of DNA metabarcoding on homogenized and digested feces and/or buccal swabs has also been shown to be effective in assessing the vertebrate and invertebrate diet profiles of not only fish predators (Guillerault et al 2017;Kumari et al 2019) but also avian (Nota et al 2019;Sow et al 2020) and mammal predators (Deagle et al 2009;Gillet et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Whose Kids Did You Eat? Genetic Identification of Species and Parents of Larval Lampreys in Fish Predator Guts

2021

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The Pacific Lamprey Entosphenus tridentatus is an important species both ecologically and culturally, but their population numbers and range have declined severely in the past several decades in the Columbia Basin. In freshwater ecosystems, larval lampreys are candidate prey species for many fish predators, but little is known regarding the magnitude of predation they currently experience. Predation on larval Pacific Lampreys may be substantially underestimated by morphological gut content analysis because their soft bodies are quickly digested and leave no hard evidence, such as bones and scales. We conducted an experimental predation study and analyzed larval Pacific Lampreys in the gut contents of predators' digestive tracts via both morphological and molecular methods. Whereas most consumed larval lampreys (67-100%) were morphologically unidentifiable within two nights, genetic species identification rates using four single nucleotide polymorphisms from stomachs were high (83%). The single nucleotide polymorphisms optimized for parentage in Pacific Lamprey also successfully identified the parents of four out of five Pacific Lampreys with complete genotypes. When a genetic baseline of candidate parents is available, this molecular technique using parentage-based tagging offers a novel method for determining precise characteristics of predation behavior in field studies, including the ages and natal origins of larvae being consumed.

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Section: Discussionmentioning

confidence: 99%

Whose Kids Did You Eat? Genetic Identification of Species and Parents of Larval Lampreys in Fish Predator Guts

2021

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“…Most diet studies using DNA metabarcoding have targeted different fish species (e.g., Bachiller et al, 2020;Kume et al, 2021;Shink et al, 2019), followed in number by studies targeting marine mammals (e.g., Granquist et al, 2018;Nielsen et al, 2007;Tverin et al, 2019) (Figure 5). Regarding regions, most studies were performed in the North Atlantic Ocean, followed by the North Pacific Ocean.…”

Section: Dna Metabarcoding For Assessing Diets From Gut Contents Faec...mentioning

confidence: 99%

Environmental Dna in an Ocean of Change: Status, Challenges and Prospects

Havermans¹,

Dischereit²,

Pantiukhin³

et al. 2022

ACMAR

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Environmental DNA (eDNA) studies have burgeoned over the last two decades and the application of eDNA has increased exponentially since 2010, albeit at a slower pace in the marine system. We provide a literature overview on marine metazoan eDNA studies and assess recent achievements in answering questions related to species distributions, biodiversity and biomass. We investigate which are the better studied taxonomic groups, geographic regions and the genetic markers used. We evaluate the use of eDNA for addressing ecological and environmental issues through food web, ecotoxicological, surveillance and management studies. Based on this state of the art, we highlight exciting prospects of eDNA for marine time series, population genetic studies, the use of natural sampler DNA, and eDNA data for building trophic networks and ecosystem models. We discuss the current limitations, in terms of marker choice and incompleteness of reference databases. We also present recent advances using experiments and modeling to better understand persistence, decay and dispersal of eDNA in coastal and oceanic systems. Finally, we explore promising avenues for marine eDNA research, including autonomous or passive eDNA sampling, as well as the combined applications of eDNA with different surveillance methods and further molecular advances. Keywords: environmental DNA, DNA metabarcoding, marine metazoa, biodiversity, population genetics, natural sampler DNA, diet analysis.

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“…After undergoing smoltification, Arctic Lampreys begin feeding on other fishes while transitioning from freshwater to salt water (Kucheryavyi et al 2007;Makhrov et al 2013;Sutton 2017). Although many lamprey species are parasitic blood feeders, Arctic Lampreys are flesh eating (Renaud et al 2009;Shink et al 2019). After some years growing at sea, Arctic Lampreys cease feeding and return to freshwater tributaries to spawn and die (Kucheryavyi et al 2007;Makhrov et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the viability of the use of T‐bar and radiotelemetry tags on prespawn Arctic Lampreys

Spanos,

Cunningham,

Drew

et al. 2023

N American J Fish Manag

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ObjectiveArctic Lampreys Lethenteron camtschaticum are harvested by subsistence and commercial fisheries in the Yukon–Kuskokwim River region of Alaska; however, baseline population data are deficient. For mark–recapture and telemetry studies to be effectively implemented to assess Arctic Lamprey abundance, migratory and dispersal patterns, and spawning locations, tags must not impact fish survival, physiology, or behavior.MethodsFor this laboratory evaluation, we examined survival, tag retention, swim endurance at short‐term (1 day posttreatment) and long‐term (43 days [~6 weeks] posttreatment) intervals, and incision healing for T‐bar‐tagged and radio‐tagged prespawn Yukon River Arctic Lampreys (N = 216). Six treatment groups were evaluated: control; sham surgery; external T‐bar anchor tag; and small (0.30 g; 0.1–0.4% tag burden [ratio of tag weight to fish body weight]), medium (0.57 g; 0.2–0.8% tag burden), and large (1.50 g; 0.6–1.9% tag burden) internal dummy radio transmitter tags.ResultAlthough all lampreys survived tagging and surgical procedures through the first 4 weeks of the experiment, a higher tag burden was associated with an increased mortality hazard up to 35 weeks postsurgery. Over the 14‐week experimental period, one T‐bar tag and one small radio tag were shed by lampreys. Although treatment was not a significant predictor of swim endurance, a higher tag burden led to reductions in swim endurance at 1 day posttreatment but not at 43 days posttreatment. Healing did not differ among surgical treatment groups, but persistent inflammation was observed at incision sites and skin erosion was observed at antenna protrusion locations.ConclusionThese results indicate that T‐bar anchor tags and internal radio tags are acceptable for Arctic Lamprey tagging studies if the radio tag burden is ≤1.3% for short‐term (≤14‐week) studies and ≤0.5% for long‐term (≥14‐week) studies.

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Utilizing DNA metabarcoding to characterize the diet of marine-phase Arctic lamprey (Lethenteron camtschaticum) in the eastern Bering Sea

Cited by 18 publications

References 47 publications

Whose Kids Did You Eat? Genetic Identification of Species and Parents of Larval Lampreys in Fish Predator Guts

Whose Kids Did You Eat? Genetic Identification of Species and Parents of Larval Lampreys in Fish Predator Guts

Environmental Dna in an Ocean of Change: Status, Challenges and Prospects

Evaluating the viability of the use of T‐bar and radiotelemetry tags on prespawn Arctic Lampreys

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