Utilizing online-dual-SPE-LC with HRMS for the simultaneous quantification of amphotericin B, fluconazole, and fluorocytosine in human plasma and cerebrospinal fluid

Qu, Lihua; Qian, Jing; Ma, Ping; Yin, Zheng

doi:10.1016/j.talanta.2016.12.052

Cited by 23 publications

(12 citation statements)

References 28 publications

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“…For this purpose there are several extraction techniques such as liquid‐liquid extraction (LLE), solid phase extraction (SPE), pressurized fluid extraction (PFE) and supercritical fluid extraction (SFE) , among which SPE has received special attention because this technique can be coupled online with HPLC‐MS/MS systems . The on‐line SPE is an efficient fully automatic sample preparation that has several advantages such as: reduced errors and analysis time; smaller amount of samples and organic solvents is necessary; reduced the contact to hazardous reagents and infectious materials; lower detection limits; improve method sensitivity and robustness when compared to off‐line SPE sample preparation .…”

Section: Introductionmentioning

confidence: 99%

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

et al. 2018

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Solid-phase extraction coupled online with high performance liquid chromatography and tandem mass spectrometry was successfully applied to determine low concentrations of ceftiofur antibiotic in bovine milk samples. A silica-anchored ionic liquid was applied as sorbent material to be used as extraction phase in the proposed online system. The material was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. In order to improve the system reproducibility, the following experimental parameters were optimized: organic solvent percentage, time and sample loading flow rate. Subsequently, the method was validated presenting satisfactory results as adequate selectivity, good linearity and correlation coefficient higher than 0.98. The limit of detection and quantification were 0.1 and 0.7 μg/L, respectively. The precision of the methodology was evaluated as repeatability and intermediate precision, with relative standard deviation values lower than 15%. The accuracy of the method ranged from 72.8 to 137% and the minimum and maximum recovery values were 73.4 and 111.3%, respectively. After the validation, seven milk samples were analyzed and although ceftiofur was not detected in any of them the method was demonstrated to be efficient when applied to the analysis of milk samples fortified with the pollutant of interest.

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Section: Introductionmentioning

confidence: 99%

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

et al. 2018

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“…One emergent strategy is the replacement of conventional mass analyzer (triple quadrupoles) by Orbitrap technology, allowing high mass resolution measurements over wider concentration ranges [8]. This high-resolution mass spectrometry approach has been implemented and tested for anticancer drugs [9,95], antivirals [96], antifungals [97], antibiotics [98,99], and psychoactive drugs [8].…”

Section: Box 2 Chromatographymentioning

confidence: 99%

On-Site Therapeutic Drug Monitoring

Ates

Roberts

Lipman

et al. 2020

Trends in Biotechnology

168

160

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Trends in Biotechnology mass spectrometry (LC-MS/MS) (see Glossary) methods. Despite its high specificity, matrix interference may lead to falsely low or high results; that is, the matrix and co-eluting compounds can interfere with the ionization process in MS (via ion suppression/enhancement) [5]. Furthermore, the throughput of LC-MS/MS is lower than that of the conventional immunoassay platforms. Recent studies are either addressing these issues [6,7] or exploiting or improving this method's inherent advantages [8,9]. Accordingly, there has been a significant effort to increase the throughput of chromatographic methods [10]. Pioneering multiplex approaches have been reported for antiretroviral agents (ARVs) [11], antifungals [12], antineoplastics [13], antibiotics [6,7,14], antidepressants [15], and immunosuppressive drugs [16] in the past decade. More recently, ultra-performance liquid chromatography (UPLC)-MS/MS has been used for simultaneous quantification of antibiotics [17] and ARVs from plasma [11] and breast milk samples [18]. Another focus is the extension of TDM studies toward unconventional samples and sampling. Several LC-MS/MS methods have been developed and applied for hair [11,18], dried blood spots [19], urine [20], sweat [21], saliva [22,23], and tissue biopsies [24].

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“…LC-MS/MS has facilitated the development of multiparameter quantitative methods, 41,42 and high-resolution MS methods without the need for tedious sample preparation and tuning parameter optimization in full-MS scan mode have been developed. 43,44…”

Section: Analyticsmentioning

confidence: 99%

Antifungal Drugs TDM: Trends and Update

Kably

Launay

Derobertmasure

et al. 2022

Therapeutic Drug Monitoring

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The increasing burden of invasive fungal infections results in growing challenges to antifungal (AF) therapeutic drug monitoring (TDM). This review aims to provide an overview of recent advances in AF TDM. Methods:We conducted a PubMed search for articles during 2016-2020 using "TDM" or "pharmacokinetics" or "drug-druginteraction" with "antifungal," consolidated for each AF. Selection was limited to English language articles with human data on drug exposure.Results: More than 1000 articles matched the search terms. We selected 566 publications. The latest findings tend to confirm previous observations in real-life clinical settings. The pharmacokinetic variability related to special populations is not specific but must be considered. AF benefit-to-risk ratio, drug-drug interaction (DDI) profiles, and minimal inhibitory concentrations for pathogens must be known to manage at-risk situations and patients. Itraconazole has replaced ketoconazole in healthy volunteers DDI studies. Physiologically based pharmacokinetic modeling is widely used to assess metabolic azole DDI. AF prophylactic use was studied more for Aspergillus spp. and Mucorales in oncohematology and solid organ transplantation than for Candida (already studied). Emergence of central nervous system infection and severe infections in immunocompetent individuals both merit special attention. TDM is more challenging for azoles than amphotericin B and echinocandins. Fewer TDM requirements exist for fluconazole and isavuconazole (ISZ); however, ISZ is frequently used in clinical situations in which TDM is recommended. Voriconazole remains the most challenging of the AF, with toxicity limiting high-dose treatments. Moreover, alternative treatments (posaconazole tablets, ISZ) are now available.Conclusions: TDM seems to be crucial for curative and/or longterm maintenance treatment in highly variable patients. TDM poses fewer cost issues than the drugs themselves or subsequent treatment issues. The integration of clinical pharmacology into multidisciplinary management is now increasingly seen as a part of patient care.

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Utilizing online-dual-SPE-LC with HRMS for the simultaneous quantification of amphotericin B, fluconazole, and fluorocytosine in human plasma and cerebrospinal fluid

Cited by 23 publications

References 28 publications

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

On-Site Therapeutic Drug Monitoring

Antifungal Drugs TDM: Trends and Update

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