Utilizing Quantitative Proteomics to Identify Species-Specific Protein Therapeutic Targets for the Treatment of Leishmaniasis

Abstract

Leishmaniasis is a tropical disease caused by Leishmania parasites, which are transmitted through the bites of infected sandflies. We focused on the emergence of leishmaniasis in Thailand caused by a species ( Leishmania orientalis ). Treatment by chemotherapy is not effective against L. orientalis . Hence, we intended to solve this issue using a proteomics approach to investigate protein profiles and in silico analysis for the identification of antigenic proteins from L. orientalis , Leishmania martiniquensis , and Leishmania donovani . Using principal component analysis (PCA), protein profile comparisons indicated that different species of Leishmania are different at the protein level. Proteomics analysis identified 6099 proteins. Among these proteins, 1065 proteins were used for further analysis. There were 16 proteins that were promising candidates for therapeutic aspects as they were abundantly expressed and common to all species. In silico analysis of protein’s antigenicity revealed that eight proteins had the potential for the development of antigenic molecules. Protein profile information and these antigenic proteins may play key roles in the pathogeny of leishmaniasis and can be used as novel therapeutic targets against leishmaniasis in the future.

Integrated transcriptomic and proteomic analyses of plerocercoid and adult Spirometra mansoni reveal potential important pathways in the development of the medical tapeworm

“…These pathways may be related to parasite growth and development and contribute to their response to stress [ 40 – 42 ]. KEGG pathway analysis is generally used to capture the systematic properties of the inner activities of cells as reference information for the integration and interpretation of high-throughput proteomics datasets [ 43 , 44 ]. Therefore, we performed KEGG pathway enrichment analysis with the aim of discovering important molecules or pathways that play key roles during S. mansoni growth and development.…”

Omics Approaches in Drug Development against Leishmaniasis: Current Scenario and Future Prospects

“…During the proteome analysis, 6099 proteins were identified, of which 1065 were used for analysis. In this study, 16 proteins were identified as therapeutic targets for all species, and an in silico analysis of protein antigenicity showed that eight proteins (E8NH10, A4IAU0, A0A640KJ19, A0A451EJM4, ndpk, A0A3S7WPD8, A4HEA3, Q4Q8G4) genes hydrophferently conveyed Pjorlar amastiogote have the potential for the development of antigenic molecules for the treatment of diseases caused by any Leishmania species [ 48 ].…”

Exploring the Role of NH₄Cl Supplementation on Triacylglycerol Production by <em>Chlamydomonas reinhardtii </em>under Mixotrophic Cultivation using a Proteomics Approach

“…The alga cells were prepared for proteomics using a previously published protocol with minor modifications [16,17]. Briefly, the cells were lysed on ice using a probe tip sonication at a frequency of 20 kHz and 80% amplitude for 2 s on and 3 s off for a total of 15 s in 200 l lysis buffer (0.2% TritonX-100, 10 mM DTT, 5 mM sodium chloride, 10 mM HEPES-KOH, pH 8.0) with protease inhibitor cocktail.…”