Utilizing the micron sized non-thermal atmospheric pressure plasma inside the animal body for the tumor treatment application

Abstract

This study aimed to evaluate the effects of micron sized non-thermal atmospheric pressure plasma inside the animal body on breast cancer tumor. The μ-plasma jet consists of micron sized hollow tube in which pure helium gas is ionized by high voltage (4 kV) and high frequency (6 kHz). The efficiency of the plasma treatment in killing cancer cells was first investigated by cell viability measurements of treated 4T1 cells using flow cytometry and cell cycle analysis. For exploration of the in vivo effects of the plasma treatment, the BALB/c mice inoculated by 4T1 cell lines were exposed subcutaneously to plasma for 3 minutes. In addition, H&E staining, TUNEL and Western blotting assays were performed in order to observed the effects of the non-thermal plasma on the tumor cells. The results showed that the efficiency of the plasma in suppression of the tumor growth is comparable to that of a typical chemotherapy drug. Moreover, the results indicated that the plasma induces apoptosis in the tumor tissue and increases the ratio of the apoptotic to anti-apoptotic protein expression. We believe that these findings presented herein may extend our knowledge of the mechanisms by which the plasma exerts its promising anti-cancer effects.

Cold atmospheric pressure plasmas in dermatology: Sources, reactive agents, and therapeutic effects

“…[ 15,121 ] The therapeutic effects of CAP are not limited to cultured cancer cells in vitro, such as breast, melanoma, and colorectal cancer cells, [ 12 ] but also have been demonstrated in animal models. [ 176,177 ]…”

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

“…Great efforts are currently made extending the range of plasma applications with different devices. This includes blood coagulation [Amritkar et al, 2013;Aleinik et al, 2017;Miyamoto et al, 2017], ophthalmology [Rosani et al, 2015;Ye et al, 2015;Recek et al, 2016], oncology [Ye et al, 2015;Mirpour et al, 2016;Binenbaum et al, 2017], and various diseases in dermatology [Daeschlein et al, 2010[Daeschlein et al, , 2011Klebes et al, 2014]. Addressing not only the efficacy but also safety of different plasma sources or their optimum operational settings, genotoxic testing is indispensable.…”

Cold Atmospheric Plasma: A Powerful Tool for Modern Medicine

“…Importantly, plasma administration for 3 min achieved tumour growth reduction comparable with chemotherapy. There was also significant change in the ratio of pro-and anti-apoptotic gene activity at the molecular level [127].…”