UV-C (254 nm) lethal doses for SARS-CoV-2

Sabino, Caetano P.; Sellera, Fábio P.; Sales-Medina, Douglas Ferreira; Machado, Rafael Rahal Guaragna; Durigon, Edison Luiz; Freitas‐Junior, Lúcio H.; Ribeiro, Martha S.

doi:10.1016/j.pdpdt.2020.101995

Cited by 71 publications

(77 citation statements)

References 6 publications

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“…To the best of our knowledge, this is the first paper on SARS-CoV-2 describing the effect of UV-C on different infected materials together with the physiological viral infectivity decay as well. As for virus survival tests, other studies previously evaluated the effect of UV-C light on SARS-CoV-2 inactivation by using different experimental conditions often consisting in higher virus inoculums (50 to 600 µL) [16][17][18]42,43] in respect to our approach that should be closer to possible scenarios of surfaces contamination. Indeed, the UV-C doses found to reduce the viral titer of about 3 logs (99.9% reduction) were much higher than ours.…”

Section: Discussionmentioning

confidence: 99%

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SARS-CoV-2 Survival on Surfaces and the Effect of UV-C Light

Gidari

Sabbatini

Bastianelli

et al. 2021

Viruses

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The aim of this study was to establish the persistence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on inanimate surfaces such as plastic, stainless steel, and glass during UV-C irradiation which is a physical means commonly utilized in sanitization procedures. The viral inactivation rate, virus half-life, and percentage of titer reduction after UV-C irradiation were assessed. Infectivity was maintained on plastic and glass until 120 h and on stainless steel until 72 h. The virus half-life was 5.3, 4.4, and 4.2 h on plastic, stainless steel, and glass, respectively. In all cases, titer decay was >99% after drop drying. UV-C irradiation efficiently reduced virus titer (99.99%), with doses ranging from 10.25 to 23.71 mJ/cm2. Plastic and stainless steel needed higher doses to achieve target reduction. The total inactivation of SARS-CoV-2 on glass was obtained with the lower dose applied. SARS-CoV-2 survival can be long lasting on inanimate surfaces. It is worth recommending efficient disinfection protocols as a measure of prevention of viral spread. UV-C can provide rapid, efficient and sustainable sanitization procedures of different materials and surfaces. The dosages and mode of irradiation are important parameters to consider in their implementation as an important means to fight the SARS-CoV-2 pandemic.

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Section: Discussionmentioning

confidence: 99%

“…UV-C light induces chemical modifications of nucleic acids, leading to the blocking of replication mechanisms [ 15 ]. SARS-CoV-2 is highly susceptible to UV-C irradiation, and different studies demonstrated the rapid inactivation of the virus on surfaces and supplies [ 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 Survival on Surfaces and the Effect of UV-C Light

Gidari

Sabbatini

Bastianelli

et al. 2021

Viruses

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“…Characteristics of the included studies are reported in Table I. Most of the studies (n = 8) were conducted in the United States [33][34][35][36][37][38][39][40], one of which also in Korea [40], four in Japan [41][42][43][44], two in Italy [45,46], and one each in China [47], Israel [48], Germany [49], and Brazil [50].…”

Section: Characteristics Of Included Studiesmentioning

confidence: 99%

“…They were published between 1972 and 2020, with three published before 2000 [34,37,44] and J o u r n a l P r e -p r o o f eight published in 2020 [35,38,39,41,43,[48][49][50]. All were experimental studies, conducted in laboratory settings: four focused on SARS-CoV-1 [36,42,45,47], one study on MERS-CoV [33], three on seasonal human coronaviruses [34,35,48] and six on SARS-CoV-2 [38,39,41,43,49,50].…”

Section: Characteristics Of Included Studiesmentioning

confidence: 99%

The efficacy of ultraviolet light-emitting technology against coronaviruses: a systematic review

Chiappa

Frascella

Vigezzi

et al. 2021

Journal of Hospital Infection

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Mitigating transmission of SARS-CoV-2 in water is thus critical in both developed or developing countries. Current chemical and germicidal ultraviolet light (i.e., UV 254 ) disinfection strategies can be well managed in centralized water and wastewater treatment plants to inactivate SARS-CoV-2 (3, 5). These strategies are almost impossible to be implemented at the household or community levels, where raw sewage, unsealed sewer vent pipes, leaking septic systems, medical biological wastes, etc.…”

Section: Introductionmentioning

confidence: 99%

In vitro rapid inactivation of SARS-CoV-2 by visible light photocatalysis using boron-doped bismuth oxybromide

Ling

Carletti

Cheng

et al. 2021

Preprint

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Inactivation of SARS-CoV-2 in wastewater and on surfaces is critical to prevent the fecal-oral and fomite transmission, respectively. We hypothesized that visible light active photocatalysts could dramatically enhance the rate or extent of virus inactivation and enable the use of visible light rather than shorter wavelength ultraviolet light. A novel visible light active photocatalyst, boron-doped bismuth oxybromide (B-BiOBr), was synthesized and tested for its SARS-CoV-2 inactivation towards Vero E6 cell lines in dark and under irradiation at 426 nm by a light emitting diode (LED) in water. SARS-CoV-2 inactivation in the presence of B-BiOBr (0.8 g/L) under LED irradiation reached 5.32-log in 5 min, which was 400 to 10,000 times higher than those achieved with conventional photocatalysts of tungsten or titanium oxide nanomaterials, respectively. Even without LED irradiation, B-BiOBr inactivated 3.32-log of SARS-CoV-2 in the dark due to the ability of bismuth ions interfering with the SARS-CoV-2 helicase function. LED irradiation at 426 nm alone, without the photocatalyst, contributed to 10% of the observed inactivation and was attributed to production of reactive oxygen species due to blue-light photoexcitation of molecules in the culture media, which opens further modes of action to engineer disinfection strategies. The visible light active B-BiOBr photocatalyst, with its rapid SARS-CoV-2 inactivation in the presence and absence of light, holds tremendous opportunities to build a healthy environment by preventing the fecal-oral and fomite transmission of emerging pathogens.

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UV-C (254 nm) lethal doses for SARS-CoV-2

Abstract: Highlights We developed a controlled assay to investigate the UV-C inactivation kinetics for SARS-CoV-2. We established the UV-C lethal doses for the virus in vitro . LD 90 for SARS-CoV-2 was reached at 0.016 mJ/cm 2 . LD 99.999 was achieved at 108.714 mJ/cm 2 with … Show more

Cited by 71 publications

References 6 publications

SARS-CoV-2 Survival on Surfaces and the Effect of UV-C Light

SARS-CoV-2 Survival on Surfaces and the Effect of UV-C Light

The efficacy of ultraviolet light-emitting technology against coronaviruses: a systematic review

In vitro rapid inactivation of SARS-CoV-2 by visible light photocatalysis using boron-doped bismuth oxybromide

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