UV protectants for Candida oleophila (strain O), a biocontrol agent of postharvest fruit diseases

Abstract

This study investigated the influence of UV-B radiation (280-320 nm) on survival of Candida oleophila strain O, an antagonist yeast that prevents postharvest diseases caused by Botrytis cinerea and Penicillium expansum on apple and pear fruits. Lethal doses (LD 50 and LD 90 ) were, respectively, 0AE89 and 1AE45 Kj m )2 for in vitro exposure and 3AE06 and 5AE5 Kj m )2 for in vivo exposure. A screening test of UV-B protectants for strain O was also evaluated under in vitro and in vivo conditions. The in vitro results showed that sodium ascorbate (0AE1% and 0AE01%), riboflavin (0AE1%) and uric acid (0AE1% and 0AE01%) were the most effective and most suitable protectants. However, only riboflavin (0AE1%) and uric acid (0AE1%) were effective under in vivo conditions. The efficacy obtained with strain O against P. expansum, when subjected to UV-B radiation, was 75AE0% and 49AE2% for pathogen concentrations of 10 5 and 10 6 spores mL, respectively. Adding riboflavin to strain O gave a similar efficacy (64AE2%). Applying strain O together with uric acid (0AE1%) was less active (47AE7%). Nonetheless, its efficacy when applied with the antioxidants sodium ascorbate (71AE1%) or ascorbic acid (82AE5%) was the greatest. Riboflavin and uric acid were the most cost-effective protectants, and could be included in the final formulation of strain O when applied preharvest.

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

“…Bacillus subtilis (Avogreen, Pretoria University, Pretoria, South Africa) was introduced in South Africa to fight the spot caused by Cercospora sp., a disease in postharvest avocado; however, it did not reach commercial success due to inconsistent results [88]. Candida oleophila (Nexy, Leasafre, Lille, France) was developed in Belgium and was presented for statutory approval in 2005 for application in postharvest phytopathogen control in citrus and banana fruit [89]. Nexy received regulatory approval in all of the European Union in 2013 [90].…”

Survival of the biological control agentCandida sakeCPA-1 on grapes under the influence of abiotic factors

“…; Lahlali et al . ), as well as the protective effect against these factors provided by different additives or adjuvants for BCA application (Cañamás et al . ; Lahlali and Jijakli ; An et al .…”

Fungal cell-wall lytic enzymes, antifungal metabolite(s) production, and characterization fromStreptomyces exfoliatusMT9 for controlling fruit-rotting fungi

“…Postharvest decay of fruits by fungal pathogens accounts for significant economic losses to the food industry worldwide [1,2]. Traditionally, synthetic fungicides are primarily applied to minimize postharvest fungal decay of fruits [3][4][5].…”