UV-Spectrophotometric Estimation of Olopatadine hydrochloride in Bulk and Pharmaceutical Dosage Form by Zero, First and Second Order Derivative Methods

Abstract

Simple and accurate UV spectrophotometric methods by Zero, First and Second order derivative method have been developed and validated for the estimation of Olopatadine hydrochloride in bulk and its pharmaceutical dosage form. The standard and sample solutions of Olopatadine hydrochloride were prepared in methanol and water. Olopatadine hydrochloride was estimated at 299, 289 and 267 nm for the derivative UV-spectrophotometric method. Beer’s law was obeyed in the concentration range of 20 to 120 μg / mL with coefficient of correlation value 0.9996, 0.999 and 0.999 for Zero, First and Second order derivative method. These methods were tested and validated for various parameters according to ICH and USP guidelines. The precision expressed as relative standard deviation were of less than 2 for the above three methods respectively. The proposed methods were successfully applied for the determination of Olopatadine hydrochloride in pharmaceutical dosage form. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.
 Keywords: Olopatadine HCl, UV-Visible spectrophotometry, Pharmaceutical Dosage forms, Derivative Spectroscopy, Method validation.

Green and sensitive detection of olopatadine in aqueous humor using a signal‐on fluorimetric approach: GREEnness assessment

“…While these methods provide outstanding detection limits and the capability to analyze OLP degradation by-products, their use is constrained by timeintensive procedures, extended pretreatment steps of the designated compounds or drug, and the need for exceptionally highpurity solvents, along with a requirement for skilled personnel. Also, OLP was detected by a few spectroscopic [34,35], and electrochemical sensors for its detection [36][37][38]. However, the sensitivity of absorbance-based techniques is limited, and the use of electrochemical sensors is hindered by the time-consuming process of synthesizing nanomaterials, such as titanium dioxide nanoparticles, for electrode modification [36].…”

A specific high‐performance thin‐layer chromatography method validated for estimation of mometasone furoate and olopatadine hydrochloride

“…Olopatadine is official in Indian Pharmacopeia [2] and United state Pharmacopeia [3]. From the literature review, it is observed that many analytical methods have been developed involving spectrophotometric [4][5][6][7][8][9][10][11][12][13][14], LC [14], HPLC [12,[15][16][17], Volttametry [20], HPTLC [15,[18][19], stability-indicating HPLC method [17] and LC-ESI-MS/MS [21] for the estimation of OLO in pharmaceutical formulations. Mometasone furoate (MOM) Figure 2 F I G U R E 2 Structure of Mometasone Furoate.…”

“…Olopatadine is official in Indian Pharmacopeia [2] and United state Pharmacopeia [3]. From the literature review, it is observed that many analytical methods have been developed involving spectrophotometric[4–14], LC [14], HPLC [12, 15–17], Volttametry [20], HPTLC [15, 18–19], stability‐indicating HPLC method [17] and LC‐ESI‐MS/MS [21] for the estimation of OLO in pharmaceutical formulations. Mometasone furoate (MOM) Figure 2, chemically [(8 S ,9 R ,10 S ,11 S ,13 S ,14 S ,16 R ,17 R )−9‐chloro‐17‐(2‐chloroacetyl)−11‐hydroxy‐10,13,16‐trimethyl‐3‐oxo‐6,7,8,11,12,14,15,16‐octahydrocyclopenta[a]phenanthren‐17‐yl] furan‐2‐carboxylate Topical glucocorticoid receptor (GR) agonist with dermatological properties.…”

Simultaneous quantification of Fluticasone propionate and Olopatadine hydrochloride by High‐performance thin‐layer chromatography

“…Olopatadine is official in Indian Pharmacopoeia [2] and US Pharmacopoeia [3]. From the literature review, it is observed that many analytical methods have been developed involving spectrophotometric [4–14], LC [14], HPLC [12, 15–17], voltammetry [20], HPTLC [15, 18–19, 22], stability‐indicating HPLC method [17], and LC‐ESI‐MS‐MS[21] for the estimation of OLO in pharmaceutical formulations. Fluticasone propionate (FLU) Figure 1, chemically [(6 S ,8 S ,9 R ,10 S ,11 S ,13 S ,14 S ,16 R ,17 R )−6,9‐difluoro‐17‐(fluoromethylsulfanyl‐carbonyl)−11‐hydroxy‐10,13,16‐trimethyl‐3‐oxo‐6,7,8,11,12,14,15,16‐octahydrocyclopenta‐[a]phenanthren‐17‐yl] propanoate Trifluorinated Gluco‐corticoid receptor with anti‐allergic and anti‐inflammatory effect [23].…”

“…official in Indian Pharmacopoeia [2] and US Pharmacopoeia [3]. From the literature review, it is observed that many analytical methods have been developed involving spectrophotometric [4][5][6][7][8][9][10][11][12][13][14], LC [14], HPLC [12,[15][16][17], voltammetry [20], HPTLC [15,[18][19]22], stabilityindicating HPLC method [17], and LC-ESI-MS-MS [21] for the estimation of OLO in pharmaceutical formulations. Fluticasone propionate (FLU) Figure 1 thren-17-yl] propanoate Trifluorinated Gluco-corticoid receptor with anti-allergic and anti-inflammatory effect [23].…”