UV Spectrophotometric Method Development and Validation of Lignocaine Hydrochloride in Bulk and Semisolid Dosage Form

Abstract

Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Lignocaine Hydrochloride in bulk and Semisolid Formulation. Methods: The UV spectrum of Lignocaine Hydrochloride in RO water showed λ max at 228.8nm. Beer’s law is valid in the concentration range of 20-100µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 20-100µg/ml with regression equation y = 0.0097x + 0.023 and regression correlation coefficient r2= 0.9992. Moreover, the method was found to be highly sensitive with LOD (3.55μg/ml) and LOQ (10.75μg/ml). Conclusion: Depending on results the given method can be successfully applied for assay of Lignocaine Hydrochloride in Semisolid formulation.

Sophisticated Planar Chromatographic Method Estimation and Validation for Newer Combination of Lidocaine HCl and Diltiazem HCl For Anal Fissure

“…Extensive study of the literature [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21], it was discovered that many analytical techniques for LID estimation had been published, including ultraviolet, high performance liquid chromatography, RP-HPLC, HPLC-MS/MS, etc. Methods for analysing DIL had been documented, including Ultra violet, high performance liquid chromatography, high performance thin layer chromatography, etc.…”

Beneath the Surface: A Case of Intussusception due to a Submucosal Lipoma in an Adult Patient

“…Lidocaine was official in British Pharmacopoeia which recommends potentiometric non-aqueous method for its analysis [4]. Literature survey reveals that numerous methods have been published for quantitative analysis of Lidocaine hydrochloride alone and in combination with other drugs such as high-performance liquid chromatography HPLC [5][6][7][8], gas chromatography [9], capillary electrophoresis [10], selective membrane electrode [11] and spectrophotometric methods [12][13][14][15]. The present work describes a new, simple spectrophotometric method for the determination of Lidocaine hydrochloride in pure form, pharmaceutical formulations and in industrial wastewater samples.…”

Validated Simultaneous Derivative Spectrophotometric Estimation of Diflunisal and Lignocaine in Bulk and Pharmaceutical Formulation

“…It is official in British Pharmacopoeia 1 . The most commonly used techniques for the determination of DIF in pharmaceutical dosage form are derivative spectrophotometry 2 , HPLC-UV detection by chemometric spectrophotometry 3 , synchronous fluorescence spectrometry [4][5] , HPLC-DAD 6 , HPLC-fluorescence detection 7 , TLC-densitometry 8 , LC-DAD 9 , LC-DAD-MS 10 , differential-pulse polarography 11 , differential-pulse and square-wave stripping voltametry 12 , capillary electrophoresis with luminescence detection 13 15 , RP-HPLC 16 , HPTLC 17 , UPLC 18 , LC-MS/MS 19 , GC-FID 20 , capillary electrophoresis with electrochemiluminescence detection 21 , partial least squares multivariate calibration 22 .…”