UV–Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 1: basic principles and properties of tyrosine chromophore

Antosiewicz, Jan; Shugar, David

doi:10.1007/s12551-016-0198-6

Cited by 62 publications

(49 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromophores have characteristic absorbance bands. Changing their environment either by adding another compound or altering the physical environment, like raising temperature, may change their energy levels and thus the wavelength and the intensity of absorbance . Band shifts to longer wavelengths (red‐shift) are bathochromic shifts and shifts to shorter wavelengths (blue‐shift) are hypsochromic shifts.…”

Section: Introductionmentioning

confidence: 99%

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

et al. 2018

View full text Add to dashboard Cite

UV-vis spectroscopy is an inexpensive, simple, flexible, non-destructive, analytical method appropriate for a wide class of organic compounds and some inorganic species. UV-vis spectrophotometers measure the absorbance or transmittance of light passing through a medium as a function of the wavelength. Chemical engineers apply it for quantitative analysis, to derive liquid phase reaction kinetics, and to identify the mechanism at the molecular scale. High performance liquid chromatography and ultra-high performance liquid chromatography integrate UV-vis detectors to identify and quantify the concentration of compounds in liquid streams. Combining these techniques with mass spectrometry facilitates identifying all species. UV-vis diffuse reflectance spectroscopy is a variant with enhanced scattering properties that measures the properties of solids and powders. A bibliometric analysis of the 10 000 most cited papers referring to UV-vis (2016 and 2017) groups research in four major clusters: nanoparticles and nanostucutres; photocatalysis and water treatment; crystals, complexes, and derivatives; and Ag and Au nanoparticles biological interaction.

show abstract

Section: Introductionmentioning

confidence: 99%

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…For HPA [Figure (A‐1)], there are two main peaks at 224 and 276 nm from the

π \to π^{*}

transition. The first transition is from

π \to π^{*}

transition of the phenyl ring while the second transition is from

n \to π^{*}

of carbonyl group in monomer structure . After polymerization, both monomer peaks exhibited a red shift by 5–10 nm with longer tail absorption to 450 nm.…”

Section: Resultsmentioning

confidence: 99%

“…p Ã of carbonyl group in monomer structure. 45 After polymerization, both monomer peaks exhibited a red shift by 5-10 nm with longer tail absorption to 450 nm. This red shift is from electron delocalization due to higher conjugated length, and formation of polymer with lower energy required for the p !…”

Section: Uv-vis Analysis Of Polyphenolsmentioning

confidence: 98%

Facile enzymatic preparation of fluorescent conjugated polymers of phenols and their application in sensing

Kiratitanavit

Bruno

Kumar

et al. 2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

The recognized drawback of utilizing metal catalysts for the synthesis of fluorescent conjugated polymers (CP) is the requirement for extensive purification to ensure complete removal of residual catalyst that would otherwise quench the fluorescence. In addition, typical synthesis of fluorescent CP involves multiple steps, monomers and solvents with varying levels of toxicity. This work demonstrates the possibility of utilizing oxidoreductase enzymes as the catalyst, for the one step polymerization of naturally occurring phenols to yield fluorescent conjugated polyphenols. The metal in the active site of the enzyme remains chelated during the synthesis allowing the polymers to be fluorescent as synthesized without the need for extensive purification. Three natural phenols, 4‐hydroxyphenylacetic acid, hydroxytyrosol, and chlorogenic acid were polymerized using Horseradish peroxidase as the biocatalyst. Spectroscopic techniques, UV–vis, Fourier transform infrared Spectroscopy–Attenuated Total Reflectance, and fluorescence, are used to characterize chemical structure and photoluminescence of these polymers. The polyphenols exhibit fluorescence with significant stokes shift in the range 30–100 nm rendering them useful in fluorescence quenching‐based sensors. Preliminary studies on use of these polymers, in the detection of nitro‐aromatic compounds in solution through using fluorescence‐quenching are also presented. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46496.

show abstract

“…Tyrosine exhibits fingerprint peaks at 280 and 230 nm due to phenol and carboxylic acid, respectively. [ 22 ] Alanine (A), an amino acid, exhibits a broad peak from 200 to 240 nm, [ 23 ] and cysteine (C), an amino acid, has a weak peak at 260 nm due to disulfide bonding. [ 24 ] The absorbance of pristine peptide film exhibits three prominent peaks at 200, 230, and 280 nm, which mainly originate from the π to π* transition in the peptide bond, [ 24 ] from the carboxylic acid in tyrosine, and from the phenol in tyrosine, respectively.…”

Section: Figurementioning

confidence: 99%

Tyrosine‐Rich Peptide Insulator for Rapidly Dissolving Transient Electronics

Namgung

Song

Sung

et al. 2020

Adv Materials Technologies

View full text Add to dashboard Cite

Transient electronics is a good platform for human implantable biomedical devices for diagnosing diseases and delivering therapeutic materials because additional surgery is not required to retrieve the device. Surrounding bio‐fluids inevitably dissolve device components, and the remaining electronic debris can trigger hazardous inflammation reactions within human body. Therefore, it is important to reduce the total dissolution time of devices even after they stop working. Thus, fast‐dissolving tyrosine‐based peptides are suggested as an insulator instead of SiO2, which has been used as a dissolution retarder in transient electronics. By combining a peptide insulator, zinc oxide semiconductor, and tungsten conductor, a biocompatible and biodegradable thin film transistor is fabricated. The device exhibits moderate performance (ON/OFF >103 and field‐effect mobility of ≈0.6 cm2 V−1 s−1) and is fast‐dissolving (<3 h) in bio‐fluids.

show abstract

UV–Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 1: basic principles and properties of tyrosine chromophore

Cited by 62 publications

References 68 publications

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

Facile enzymatic preparation of fluorescent conjugated polymers of phenols and their application in sensing

Tyrosine‐Rich Peptide Insulator for Rapidly Dissolving Transient Electronics

Contact Info

Product

Resources

About