UVA/UVB-induced genotoxicity and lesion repair in Colossoma macropomum and Arapaima gigas Amazonian fish

Groff, Aline Aparecida; Silva, Juliana da; Nunes, Emilene A.; Ianistcki, Martus; Guecheva, Temenouga Nikolova; Oliveira, Alzira Miranda de; Oliveira, Christiane Patricia Feitosa de; Val, Adalberto Luı́s; Henriques, João Antônio Pêgas

doi:10.1016/j.jphotobiol.2010.02.011

Cited by 36 publications

(26 citation statements)

References 36 publications

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“…UV carries more energy per photon than any other wavelength reaching the earth. Such high energy photon can potentially damage many biological molecules [1]. Of the three type of incoming UV radiation, UV-B (280-320 nm) remained stressful to the terrestrial plants and animals including human [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…If DNA damage is not repaired, cells undergo complex enzymatic reactions expends high energy that might lead to apoptosis, necrosis or other forms of cell death [16]. The alterations most frequently induced are cyclobutane pyrimidine dimers (CPDs) and pyrimidine pyrimodone photoproducts [17,18,1]. Some ROS-induced protein modifications can result in unfolding or alteration of protein structure, and some are essentially harmless events.…”

Section: Introductionmentioning

confidence: 99%

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Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Singh

Sharma

Chakrabarti

2015

Journal of Photochemistry and Photobiology B: Biology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Singh

Sharma

Chakrabarti

2015

Journal of Photochemistry and Photobiology B: Biology

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“…Specimens contaminated by this pesticide when maintained in water free of pollution also showed the ability to repair DNA damage (MOHANTY et al, 2011). Using the comet assay, Groff et al (2010) detected a reduction in DNA damage to the erythrocytes of Neotropical fish (Arapaima gigas and Colossoma macroporum) 12 hours after exposure to UVR-B (ultraviolet radiation B) and suggest that nucleotide excision repair is involved in DNA repair.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genotoxicity and depuration of anthracene in the juvenile coastal fish Trachinotus carolinus using the comet assay

et al. 2013

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A B S T R A C TIn the environment, anthracene is characterized as being persistent, bioaccumulative and toxic to aquatic organisms. Biotransformation of xenobiotic substances, such as anthracene, produces reactive oxygen species that may induce DNA strand breaks. The aim of the present study was to evaluate the DNA damage in juvenile T. carolinus exposed to different concentrations (8, 16 and 32 µg.L -1 ) of anthracene for 24 h in the dark then subsequently allowed to depurate in clean water for different periods of time (48, 96 or 144 h) using the comet assay. Our results show that anthracene is genotoxic to T. carolinus and that DNA damage was dose-and depuration/time-dependent. Anthracene genotoxicity was observed in all experimental concentrations. Depuration seemed to be more efficient in fish exposed to the lowest anthracene concentration and maintained in clean water for 96 h. R E S U M OAmbientalmente, o antraceno é considerado persistente, bioacumulativo e tóxico para organismos aquáticos. A biotransformação de substâncias xenobióticas, como o antraceno, produz espécies reativas de oxigênio que podem agir sobre o DNA. O objetivo deste trabalho foi avaliar o dano ao DNA em T. carolinus expostos a diferentes concentrações de antraceno (8, 16 e 32 µg.L -1 ) por 24 h no escuro e subsequentemente mantidos em água limpa para depuração por três diferentes períodos de tempo (48, 96 e 144 h) através de ensaio cometa. Os resultados obtidos demonstram que o antraceno é genotóxico para T. carolinus e o dano ao DNA foi dose-e tempo de depuração-dependente. A genotoxicidade do antraceno foi detectada nas três concentrações utilizadas. A depuração pareceu ser mais eficiente nos peixes expostos a menor concentração de antraceno e mantidos por 96 h em água limpa.

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“…Several studies have demonstrated the value of micronuclei as biomarkers and fish as sentinel organisms (e.g., Andrade et al 2004;Ferraro et al 2004;Souza and Fontanetti 2006). In addition, studies on genotoxicity indicate that C. macropomum has potential as an experimental model for monitoring aquatic ecosystems (Chapadense et al 2009;Groff et al 2010;Rocha et al 2011). The frequency of micronuclei and morphological nuclear abnormalities are effective tools for analyzing the health status of fish (Noga 2000;Webb and Gagnon 2002).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and hematological parameters in Colossoma macropomum (Pisces, Serrasalmidae) as biomarkers for environmental impact assessment in a protected area in northeastern Brazil

Neta

Sousa

Sobrinho

et al. 2015

Environ Sci Pollut Res

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Genotoxic and hematological parameters in tambaqui (Colossoma macropomum), a native freshwater fish, were used as biomarkers to assess exposure to environmental stressors within the Maracanã Protected Area of Maranhão State, Brazil. Fish were sampled at two sites-Serena Lagoon (control) and Ambude River-on four occasions (dry and rainy season), and biometric data (length and weight) recorded and blood collected from all fish for analysis. Erythrocyte indices-mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration-were calculated. Blood samples were stained with Giemsa and acridine orange, and microscopically examined for micronuclei and morphological nuclear abnormalities. Micronuclei were observed in fish from both sites, although the frequency was significantly higher in fish from the Ambude River and morphological nuclear abnormalities were only observed in fish from the Ambude River. More morphological nuclear abnormalities and a larger number of micronuclei were observed in erythrocytes stained with acridine orange compared with those stained with Giemsa. On average, erythrocyte indices were lower in fish from the Ambude River than from the Serena Lagoon. The results confirm that genotoxic and hematological parameters in C. macropomum can be used as indicators of environmental health and could be valuable tools for monitoring environmental conditions within protected areas.

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UVA/UVB-induced genotoxicity and lesion repair in Colossoma macropomum and Arapaima gigas Amazonian fish

Cited by 36 publications

References 36 publications

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Assessment of genotoxicity and depuration of anthracene in the juvenile coastal fish Trachinotus carolinus using the comet assay

Genotoxic and hematological parameters in Colossoma macropomum (Pisces, Serrasalmidae) as biomarkers for environmental impact assessment in a protected area in northeastern Brazil

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